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0.05). However, the interaction of calcium carbonate with cadmium on the serum level of ALP enzyme was significant and with increasing both variables, the amount of ALP enzyme in crab hemolymph increased (P <0.05). The present study also showed that the interaction of all three variables on CW/CHD and CW/CHL ratio of male crabs was not significant (P> 0.05), but the interaction of all three variables on CW/MBDWt of male and female crabs was significant at a 95% confidence level (P <0.05). According to the values obtained in finding the optimal points of the studied variables on the maximum morphological ratios and the optimal level of ALP and ALT enzymes, recommend using high rates of desirability (80-95%) with temperature 18-20°C, Calcium carbonate hardness about 240-311 mg/l, And the absence of cadmium in the aqueous medium.]]>
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