Journal of Fisheries 2008-5729 65 2 2012 07 22 Biochemical Characteristics of Blood and Histopathological Study of Experimental Diazinon Poisoning in Common Carp (Cyprinus carpio) Biochemical Characteristics of Blood and Histopathological Study of Experimental Diazinon Poisoning in Common Carp (Cyprinus carpio) 119 133 28619 10.22059/jfisheries.2012.28619 FA Alireza Mirvaghefi 0000-0002-8620-501X Hamid Farahmand Gholamreza Rafiee Mahdi Banaee Journal Article Diazinon is commonly used for pest control in the agricultural farms surrounding freshwater reservoirs. Therefore, this study was conducted to determine the sub-lethal toxicity of this organophosphorous pesticide, in aquatic ecosystems as a pollutant, and its effects on histopathology of liver and some biochemical blood parameters of common carp, Cyprinus carpio. Diazinon was applied at sub-lethal concentrations of 60 and 120 µg/L during 10, 20 and 30 days. Compared to the control specimens, fish after a chronic exposure to diazinon had significantly (p<0.05) higher plasma Aspartat transaminase (AST), Alanin transaminase (ALT), alkaline phosphatase (ALP), lactate dehyrogenase (LDH), cretinin phosphokinase (CK) activities, and levels of glucose, albumin and cholesterol, but no significantly differences in total protein and globulin levels were observed between treated fish and control group. The histhopathological effects of diazinon on liver tissue of C.carpio, were examined by light microscopy. Disorientation of liver cells, cytoplasmic and nuclear material deposition, vacuoles formation in liver tissue, liver cell necrosis and destruction of the structure and rupture of the blood sinuses were observed in the liver tissue of treated fish by diazinon. Diazinon is commonly used for pest control in the agricultural farms surrounding freshwater reservoirs. Therefore, this study was conducted to determine the sub-lethal toxicity of this organophosphorous pesticide, in aquatic ecosystems as a pollutant, and its effects on histopathology of liver and some biochemical blood parameters of common carp, Cyprinus carpio. Diazinon was applied at sub-lethal concentrations of 60 and 120 µg/L during 10, 20 and 30 days. Compared to the control specimens, fish after a chronic exposure to diazinon had significantly (p<0.05) higher plasma Aspartat transaminase (AST), Alanin transaminase (ALT), alkaline phosphatase (ALP), lactate dehyrogenase (LDH), cretinin phosphokinase (CK) activities, and levels of glucose, albumin and cholesterol, but no significantly differences in total protein and globulin levels were observed between treated fish and control group. The histhopathological effects of diazinon on liver tissue of C.carpio, were examined by light microscopy. Disorientation of liver cells, cytoplasmic and nuclear material deposition, vacuoles formation in liver tissue, liver cell necrosis and destruction of the structure and rupture of the blood sinuses were observed in the liver tissue of treated fish by diazinon. https://jfisheries.ut.ac.ir/article_28619_81e2631c91b78268005021e704312097.pdf