Journal of Fisheries 2008-5729 69 2 2016 08 22 Diversity and Genetic Structure of Capoeta trutta (Heckel, 1843) Populations in the Kurdistan Province using Inter Simple Sequence Repeat Markers Diversity and Genetic Structure of Capoeta trutta (Heckel, 1843) Populations in the Kurdistan Province using Inter Simple Sequence Repeat Markers 265 273 59856 10.22059/jfisheries.2016.59856 FA Behroz Mirzaei1 1. Msc of Fisheries, Department of Fisheries, Faculty of Natural Resources, University of Zabol, Zabol, Iran Ebrahim Alizadeh Doughikollaee2 2. Associate Professor, Department of Fisheries, Faculty of Natural Resources, University of Zabol, Zabol, Iran Barzan Bahrami Kamangar3* 3. Assistant Professor, Department of fisheries, Faculty of Natural Resources, University of Kurdistan, Sanandaj, Iran Ali Arshadi4 4. Assistant Professor, Department of Fisheries, Faculty of Natural Resources, University of Zabol, Zabol, Iran Journal Article 2016 12 23 Inter Simple Sequence Repeat (ISSR) analysis was performed to evaluate genetic diversity and genetic structure of six Capoeta trutta populations from two main watersheds in the Kurdistan province. Fish samples were caught from the Choman, Shooei, Garmab Rivers in the Choman watershed and from the Gheshlagh, Gavarood, Sirvan Rivers in the Sirvan watershed. From six used dinucleotide primers, three ((AG)9C, (GA)9C and (AC)9T)) generated discernible bands. Fifty six clear and repeatable bands were generated throughout six populations. The percentages of polymorphism were 80.00, 82.98, 82.61, 85.71, 79.60 and 80.49 within the Sirvan, Gavarood, Gheshlagh, Choman, Garmab and Shooei populations, respectively. The average values of heterozygosites were determined as moderate to high within the studied populations. The intrapopulation genetic diversity indices were significantly higher in the Choman population than others. The analysis of molecular variance revealed 89 percent variation within populations whereas only 11 percent between populations. Population comparisons based on fixation index (Fst) revealed low to moderate differentiation between six studied populations. However there was no significant differentiation between populations as testified by exact test. The results showed that environmental conditions to maintain genetic diversity of Capoeta trutta populations in two watersheds are moderate. Inter Simple Sequence Repeat (ISSR) analysis was performed to evaluate genetic diversity and genetic structure of six Capoeta trutta populations from two main watersheds in the Kurdistan province. Fish samples were caught from the Choman, Shooei, Garmab Rivers in the Choman watershed and from the Gheshlagh, Gavarood, Sirvan Rivers in the Sirvan watershed. From six used dinucleotide primers, three ((AG)9C, (GA)9C and (AC)9T)) generated discernible bands. Fifty six clear and repeatable bands were generated throughout six populations. The percentages of polymorphism were 80.00, 82.98, 82.61, 85.71, 79.60 and 80.49 within the Sirvan, Gavarood, Gheshlagh, Choman, Garmab and Shooei populations, respectively. The average values of heterozygosites were determined as moderate to high within the studied populations. The intrapopulation genetic diversity indices were significantly higher in the Choman population than others. The analysis of molecular variance revealed 89 percent variation within populations whereas only 11 percent between populations. Population comparisons based on fixation index (Fst) revealed low to moderate differentiation between six studied populations. However there was no significant differentiation between populations as testified by exact test. The results showed that environmental conditions to maintain genetic diversity of Capoeta trutta populations in two watersheds are moderate.

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