Journal of Fisheries 2008-5729 79 1 2026 02 20 Replacing of fish meal with different levels of the black soldier fly (Hermetia illucens) larva meal in the diet of rainbow trout (Oncorhynchus mykiss) and their effects on growth, immune, and antioxidant indices Replacing of fish meal with different levels of the black soldier fly (Hermetia illucens) larva meal in the diet of rainbow trout (Oncorhynchus mykiss) and their effects on growth, immune, and antioxidant indices 1 15 105977 10.22059/jfisheries.2026.405572.1470 FA Mehrnaz Heybatian Fisheries Department, Faculty of Natural Resources, University of Guilan, Sowmeh Sara, Iran. Sajjadi Mirmasoud Fisheries Department, Faculty of Natural Resources, University of Guilan, Sowmeh Sara, Iran. Mehrdad Fatollahi Department of Fisheries, Faculty of Natural Resources and Earth Sciences, Shahrekord University, Shahrekord, Iran. Bahram Falahatkar Fisheries Department, Faculty of Natural Resources, University of Guilan, Sowmeh Sara, Iran. Roghieh Safari Department of Fisheries and Aquatic Sciences, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran. 0000-0002-7270-8548 Ghasem Ashouri National Artemia Research Center, Iranian Fisheries Scienc Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Urmia, Iran. Journal Article 2025 11 04 The present study aimed to evaluate the effects of replacing different levels of fishmeal with black soldier fly (BSFL) (Hermetia illucens) larva meal in the diet on growth performance, immune responses and antioxidant activity of rainbow trout (Oncorhynchus mykiss) fry. A total of 180 fry with an initial weight of 5 ± 0.3 g were randomly distributed into four treatments and three replicates. The treatments consisted of control (C; standard fishmeal-based diet) and three experimental diets in which fish meal was replaced with insect meal at inclusion levels of 10% (BSFL10), 20% (BSFL20), and 30% (BSFL30). Growth assessment across two-week intervals revealed that black soldier fly larva meal replacement significantly affected growth indices during the experiment (P<0.05). The results at the end of the experimental period showed that 10% replacement treatment resulted in a significant increase in weight gain (WG), specific growth rate (SGR), improved feed conversion ratio (FCR), body weight gain (BWI) and protein efficiency ratio (PER) compared to the control group (P<0.05), while the 20% and 30% replacement levels exhibited no significant differences in growth indices compared to control diet (P>0.05). In addition, the activities of lysozyme (LYZ) and catalase (CAT) enzymes at a replacement level of 10% were also significantly higher than the control and 30% replacement treatments (P<0.05). Complement activity (ACH50) was higher at 10% replacement level than other treatments (P<0.05). The results of this study showed that replacing black soldier fly larva at a level of 10% with fish meal is more effective in growth performance of rainbow trout fry, while increasing the replacement level up to 30% had a negative effect on growth, immune, and physiological indices. These findings suggest that black soldier fly larva meal can be used as a reliable and stable protein source with favorable performance in rainbow trout diets. The present study aimed to evaluate the effects of replacing different levels of fishmeal with black soldier fly (BSFL) (Hermetia illucens) larva meal in the diet on growth performance, immune responses and antioxidant activity of rainbow trout (Oncorhynchus mykiss) fry. A total of 180 fry with an initial weight of 5 ± 0.3 g were randomly distributed into four treatments and three replicates. The treatments consisted of control (C; standard fishmeal-based diet) and three experimental diets in which fish meal was replaced with insect meal at inclusion levels of 10% (BSFL10), 20% (BSFL20), and 30% (BSFL30). Growth assessment across two-week intervals revealed that black soldier fly larva meal replacement significantly affected growth indices during the experiment (P<0.05). The results at the end of the experimental period showed that 10% replacement treatment resulted in a significant increase in weight gain (WG), specific growth rate (SGR), improved feed conversion ratio (FCR), body weight gain (BWI) and protein efficiency ratio (PER) compared to the control group (P<0.05), while the 20% and 30% replacement levels exhibited no significant differences in growth indices compared to control diet (P>0.05). In addition, the activities of lysozyme (LYZ) and catalase (CAT) enzymes at a replacement level of 10% were also significantly higher than the control and 30% replacement treatments (P<0.05). Complement activity (ACH50) was higher at 10% replacement level than other treatments (P<0.05). The results of this study showed that replacing black soldier fly larva at a level of 10% with fish meal is more effective in growth performance of rainbow trout fry, while increasing the replacement level up to 30% had a negative effect on growth, immune, and physiological indices. These findings suggest that black soldier fly larva meal can be used as a reliable and stable protein source with favorable performance in rainbow trout diets.

https://jfisheries.ut.ac.ir/article_105977_4cd0a6d137bae97c70c0f3f953ddae47.pdf

Journal of Fisheries 2008-5729 79 1 2026 02 20 Isolation and Identification of probiotic yeasts and Bacillus species from the gastrointestinal tract of juvenile Beluga Sturgeon (Huso huso) Isolation and Identification of probiotic yeasts and Bacillus species from the gastrointestinal tract of juvenile Beluga Sturgeon (Huso huso) 17 26 105979 10.22059/jfisheries.2026.394100.1457 FA Hojatollah Jafaryan Department of Fisheries, Faculty of Agriculture and Natural Resources, Gonbad Kavous University, Gonbad Kavous, Iran. Mehdi Dehghan Department of Fisheries, Faculty of Agriculture and Natural Resources, Gonbad Kavous University, Gonbad Kavous, Iran. Mehran Habibi Rezaei Department of Biology, Faculty of Sciences, University of Tehran, Tehran, Iran. Mohammad Ali Amoozegar Department of Biology, Faculty of Sciences, University of Tehran, Tehran, Iran. Journal Article 2025 07 31 The present study aimed to develop a comprehensive protocol for the optimal isolation and identification of indigenous probiotics from the gastrointestinal tract of aquatic species. To this end, intestinal mucus samples were collected from four periods of the gastrointestinal tract of juvenile beluga sturgeons (Huso huso) with an average weight of 89.9±19.2 g and an average length of 12.78±1.24 cm. After preliminary preparation, the samples were cultured on appropriate media. The isolated strains were then identified based on morphological and physiological characteristics, DNA amplification, gene sequencing, and phylogenetic data analysis. According to the results, Bacillus licheniformis, Bacillus subtilis, and the yeast Saccharomyces cerevisiae were isolated and identified from the intestinal samples of the juvenile fish. Overall, the developed protocols demonstrated efficiency for the isolation and selection of probiotic strains, suggesting their potential applicability to other aquatic species as well. The present study aimed to develop a comprehensive protocol for the optimal isolation and identification of indigenous probiotics from the gastrointestinal tract of aquatic species. To this end, intestinal mucus samples were collected from four periods of the gastrointestinal tract of juvenile beluga sturgeons (Huso huso) with an average weight of 89.9±19.2 g and an average length of 12.78±1.24 cm. After preliminary preparation, the samples were cultured on appropriate media. The isolated strains were then identified based on morphological and physiological characteristics, DNA amplification, gene sequencing, and phylogenetic data analysis. According to the results, Bacillus licheniformis, Bacillus subtilis, and the yeast Saccharomyces cerevisiae were isolated and identified from the intestinal samples of the juvenile fish. Overall, the developed protocols demonstrated efficiency for the isolation and selection of probiotic strains, suggesting their potential applicability to other aquatic species as well.

https://jfisheries.ut.ac.ir/article_105979_6e3d4d721cda99fd06eef33554f99031.pdf

Journal of Fisheries 2008-5729 79 1 2026 02 20 Assessment of catch per unit effort (CPUE) trends of sardine fishes using purse seine fishing vessels in the fishing grounds of Hormozgan Province Assessment of catch per unit effort (CPUE) trends of sardine fishes using purse seine fishing vessels in the fishing grounds of Hormozgan Province 27 38 105978 10.22059/jfisheries.2026.393787.1456 FA Seyed Yousef Paighambari Department of Fisheries, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran. Parviz Zare Department of Fisheries, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran. Soosan Mohammadshafi Department of Fisheries, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran. Reza Abaspur Naderi Deputy of Fishing and Fishing Ports, Iranian Fisheries Organization, Tehran, Iran. Journal Article 2025 04 28 The present study investigates the annual and seasonal variations in catch per unit effort (CPUE) of sardine species caught by two‑boat purse seine fishing gear in the fishing grounds of Hormozgan Province, including the three regions of Qeshm Island, Lengeh port, and Jask port, using fisheries catch data provided by the Iran Fisheries Organization over the period 2018-2022. The results of two‑way analysis of variance (ANOVA) indicated that both fishing area and sampling year had a statistically significant effect on CPUE (P<0.05), whereas the interaction between these two factors was not significant (P>0.05). The findings also showed an overall increasing trend in sardine catches across the three studied regions during the study period. The mean CPUE (±95% confidence interval) of sardine species in the waters of Qeshm Island, Lengeh port, and Jask port was estimated at 1016±158.6, 1146±296.3, and 631±159.9 kg per haul, respectively. CPUE values in Lengeh port were significantly higher than those recorded in Jask port, while no significant difference was observed between Lengeh port and Qeshm Island. Although the highest total catch was recorded in the waters of Qeshm Island, its CPUE was lower than that of Lengeh port. The lowest CPUE of sardine species was observed in 2021, with a value of 403±109.9 kg per haul, which did not differ significantly from the CPUE recorded in 2022 (772±136.1 kg per haul), but was significantly lower than the values observed in 2018 (1202±233.4 kg per haul), 2019 (1235±166.1 kg per haul), and 2020 (1089±232.3 kg per haul) (P<0.05). Overall, the difference between observed CPUE and standardized CPUE ranged from 1 kg per haul (Qeshm Island, 2020) to 291 kg per haul (Lengeh port, 2020), with 53% of cases showing relatively substantial differences ranging between 175 and 291 kg per haul. These results indicate that CPUE values are strongly influenced by fishing area and year of exploitation. The findings of the present study can provide valuable information for the monitoring, control, and management of small pelagic fish stocks, particularly sardine species, in the southern waters of Iran. The present study investigates the annual and seasonal variations in catch per unit effort (CPUE) of sardine species caught by two‑boat purse seine fishing gear in the fishing grounds of Hormozgan Province, including the three regions of Qeshm Island, Lengeh port, and Jask port, using fisheries catch data provided by the Iran Fisheries Organization over the period 2018-2022. The results of two‑way analysis of variance (ANOVA) indicated that both fishing area and sampling year had a statistically significant effect on CPUE (P<0.05), whereas the interaction between these two factors was not significant (P>0.05). The findings also showed an overall increasing trend in sardine catches across the three studied regions during the study period. The mean CPUE (±95% confidence interval) of sardine species in the waters of Qeshm Island, Lengeh port, and Jask port was estimated at 1016±158.6, 1146±296.3, and 631±159.9 kg per haul, respectively. CPUE values in Lengeh port were significantly higher than those recorded in Jask port, while no significant difference was observed between Lengeh port and Qeshm Island. Although the highest total catch was recorded in the waters of Qeshm Island, its CPUE was lower than that of Lengeh port. The lowest CPUE of sardine species was observed in 2021, with a value of 403±109.9 kg per haul, which did not differ significantly from the CPUE recorded in 2022 (772±136.1 kg per haul), but was significantly lower than the values observed in 2018 (1202±233.4 kg per haul), 2019 (1235±166.1 kg per haul), and 2020 (1089±232.3 kg per haul) (P<0.05). Overall, the difference between observed CPUE and standardized CPUE ranged from 1 kg per haul (Qeshm Island, 2020) to 291 kg per haul (Lengeh port, 2020), with 53% of cases showing relatively substantial differences ranging between 175 and 291 kg per haul. These results indicate that CPUE values are strongly influenced by fishing area and year of exploitation. The findings of the present study can provide valuable information for the monitoring, control, and management of small pelagic fish stocks, particularly sardine species, in the southern waters of Iran. https://jfisheries.ut.ac.ir/article_105978_632a0fb1ad2492f6e8b5d25b921ba1ca.pdf

Journal of Fisheries 2008-5729 79 1 2026 02 20 Effects of dietary-house cricket meal (Acheta domesticus) in the diet on growth, body biochemical composition and digestive activity of juvenile trout (Oncorhynchus mykiss) Effects of dietary-house cricket meal (Acheta domesticus) in the diet on growth, body biochemical composition and digestive activity of juvenile trout (Oncorhynchus mykiss) 39 50 105974 10.22059/jfisheries.2026.405989.1471 FA Hadi Asadi International Sturgeon Research Institute, Iranian Fisheries Science Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Rasht, Iran. Ayoub Yousefi Jordehi International Sturgeon Research Institute, Iranian Fisheries Science Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Rasht, Iran. Ali Naghi Sarpanah International Sturgeon Research Institute, Iranian Fisheries Science Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Rasht, Iran. Mir Hamed Seyed Hassanii International Sturgeon Research Institute, Iranian Fisheries Science Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Rasht, Iran. Journal Article 2025 11 11 In recent years, insect meal has been recognized as a new, sustainable and economical protein source by researchers and has attracted the attention of producers. The present study was conducted to investigate the effects of different levels of house cricket (Acheta domesticus) meal on growth performance, biochemical composition and digestive activity of rainbow trout fry. For the experiment, four diets with different amounts of 0% (control treatment), 25% (Ad25), 50% (Ad50) and 75% (Ad75) house cricket meal were formulated instead of fish meal. The diets were distributed in tanks in a completely randomized design in 4 treatments and three replications and 300 fish with an average initial weight (5.07±0.31 g) were distributed for 8 weeks and feeding was done three times a day until satiety. Physicochemical factors such as temperature, dissolved oxygen and pH were kept constant during the experiment. The results showed that the maximum final weight in the 50% treatment was 616.20±34.89% and a significant difference was observed between the zero, 25 and 75% treatments (P<0.05). In the 75% treatment, a significant decrease in growth and feed efficiency indices was observed compared to the control (P<0.05). The maximum protein content in the 50% treatment was also significantly different from the control. The fat and moisture content of the carcass in the experimental treatments were significantly different compared to control (P<0.05). The highest activity of protease and amylase enzymes recorded in fish fed the Ad50 diet. The results of this study showed that adding cricket meal instead of fish meal up to 50% can lead to the best growth performance, body biochemical compositions and digestive enzymes. In recent years, insect meal has been recognized as a new, sustainable and economical protein source by researchers and has attracted the attention of producers. The present study was conducted to investigate the effects of different levels of house cricket (Acheta domesticus) meal on growth performance, biochemical composition and digestive activity of rainbow trout fry. For the experiment, four diets with different amounts of 0% (control treatment), 25% (Ad25), 50% (Ad50) and 75% (Ad75) house cricket meal were formulated instead of fish meal. The diets were distributed in tanks in a completely randomized design in 4 treatments and three replications and 300 fish with an average initial weight (5.07±0.31 g) were distributed for 8 weeks and feeding was done three times a day until satiety. Physicochemical factors such as temperature, dissolved oxygen and pH were kept constant during the experiment. The results showed that the maximum final weight in the 50% treatment was 616.20±34.89% and a significant difference was observed between the zero, 25 and 75% treatments (P<0.05). In the 75% treatment, a significant decrease in growth and feed efficiency indices was observed compared to the control (P<0.05). The maximum protein content in the 50% treatment was also significantly different from the control. The fat and moisture content of the carcass in the experimental treatments were significantly different compared to control (P<0.05). The highest activity of protease and amylase enzymes recorded in fish fed the Ad50 diet. The results of this study showed that adding cricket meal instead of fish meal up to 50% can lead to the best growth performance, body biochemical compositions and digestive enzymes.

https://jfisheries.ut.ac.ir/article_105974_559fc337f322affc576573fdbf14d2c6.pdf

Journal of Fisheries 2008-5729 79 1 2026 02 20 Effect of different levels of dietary olive pomace (Olea europaea) on growth performance, carcass composition, blood indices and sensory evaluation of grass carp (Ctenopharyngodon idella) Effect of different levels of dietary olive pomace (Olea europaea) on growth performance, carcass composition, blood indices and sensory evaluation of grass carp (Ctenopharyngodon idella) 51 65 105965 10.22059/jfisheries.2026.401683.1468 FA Alireza Azarmmehr Fisheries Department, Faculty of Natural Resources, University of Guilan, Sowmeh Sara, Guilan, Iran. Majidreza Khoshkholgh Fisheries Department, Faculty of Natural Resources, University of Guilan, Sowmeh Sara, Guilan, Iran. Majid Mosapour Shajani Fisheries Department, Faculty of Natural Resources, University of Guilan, Sowmeh Sara, Guilan, Iran. Akbar Banavreh Islamic Azad University, Rasht Branch, Iran. Journal Article 2025 10 04 This study was aimed to evaluate the effects of different levels of dietary-olive pomace on the growth performance, carcass composition, hematological parameters and sensory quality of grass carp (Ctenopharyngodon idella). For this purpose, a total of 225 fish with an average initial body weight of 325.90 ± 9.39 g were randomly assigned to five experimental groups (three replicates) and were reared in 1000-liter oval tanks for a 67 -day period. The treatments were included a control group (OP0: a feed without olive pomace as control), and four experimental diets containing 5% OP (OP5), 10% OP (OP10), 15% OP (OP15) and 20% OP (OP20), respectively. At the termination of the experiment, fish from each tank were randomly sampled for proximate analysis. The results demonstrated that the final body weight and weight gain were significantly higher in the OP20 group compared to other groups (P<0.05). Specific growth rate and body weight were significantly higher in the OP15 and OP20 groups compared to other groups at the end of the experiment. Furthermore, the feed conversion ratio was lower in the OP20 group. Carcass composition and moisture contents were significantly higher in the OP15 group. Crude fat contents were significantly higher in OP5 and OP20 groups as well. The ash content was also significantly decreased in the OP20 group. However, no significant differences were observed in the hematological parameters. Sensory evaluation of fillets revealed that aroma and flavor scores were lower in the OP0 and OP5 groups compared to all other groups, with the highest scores observed in the OP15 and OP20 groups. As a conclusion, the findings of this study suggest that the inclusion of OP in the diet of grass carp, particularly at a 15% level, positively influences growth performance and fillet sensory properties of grass carp. This study was aimed to evaluate the effects of different levels of dietary-olive pomace on the growth performance, carcass composition, hematological parameters and sensory quality of grass carp (Ctenopharyngodon idella). For this purpose, a total of 225 fish with an average initial body weight of 325.90 ± 9.39 g were randomly assigned to five experimental groups (three replicates) and were reared in 1000-liter oval tanks for a 67 -day period. The treatments were included a control group (OP0: a feed without olive pomace as control), and four experimental diets containing 5% OP (OP5), 10% OP (OP10), 15% OP (OP15) and 20% OP (OP20), respectively. At the termination of the experiment, fish from each tank were randomly sampled for proximate analysis. The results demonstrated that the final body weight and weight gain were significantly higher in the OP20 group compared to other groups (P<0.05). Specific growth rate and body weight were significantly higher in the OP15 and OP20 groups compared to other groups at the end of the experiment. Furthermore, the feed conversion ratio was lower in the OP20 group. Carcass composition and moisture contents were significantly higher in the OP15 group. Crude fat contents were significantly higher in OP5 and OP20 groups as well. The ash content was also significantly decreased in the OP20 group. However, no significant differences were observed in the hematological parameters. Sensory evaluation of fillets revealed that aroma and flavor scores were lower in the OP0 and OP5 groups compared to all other groups, with the highest scores observed in the OP15 and OP20 groups. As a conclusion, the findings of this study suggest that the inclusion of OP in the diet of grass carp, particularly at a 15% level, positively influences growth performance and fillet sensory properties of grass carp.

https://jfisheries.ut.ac.ir/article_105965_2ee3468f7ad9432ee1c6a77a81c15596.pdf

Journal of Fisheries 2008-5729 79 1 2026 02 20 Assessment of fecundity rates in Alburnus sellal and A. atropatenae and their relationships with length, weight and age in Tigris and Urmia Lake basins of Iran Assessment of fecundity rates in Alburnus sellal and A. atropatenae and their relationships with length, weight and age in Tigris and Urmia Lake basins of Iran 67 81 105976 10.22059/jfisheries.2026.409897.1476 FA Keyvan Abbasi Ranjbar Inland Waters Aquaculture Research Center, Iranian Fisheries Sciences Research Institute, Agricultural Research, Education and Extension Organization, Bandar Anzali, Iran. 0000-0001-5095-2905 Zaniar Ghafouri Department of Fisheries, Faculty of Natural Resources, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran. Yaseman Sayyadoghli Department of Marine Biology, Faculty of Science, University of Guilan, Rasht, Iran. Journal Article 2025 12 21 Alburnus sellal and A. atropatenae belong to Leuciscidae family, and are found in southwestern and Urmia Lake basins of Iran, respectively. These fish have ecological and sport fishing values as well. The main purpose of this study was to investigate the relationship between the fecundity with length, weight and age in Gamasiab, Ghaleh-Chai and Mahabad-Chai Rivers. For this purpose, fish sampling was performed in 2008 and 2011 using electrofishing device and Cast-net. The results of the investigation on 47 spicemens of female A. sellal and 37 spicemens of A. atropatenae showed the total absolute fecundity (sum of large, medium and small eggs) in the A. sellal, is 1160-13021 (5571.7±3037.3, average±S.D) and for A. atropatenae, 1205-6588 (3113.2±1252). The total relative fecundity was estimated 125-507 (263.3±86.1) in A. sellal and 144-297 (197.8±41.1) in A. atropatenae per gram of body weight. The correlation (r2) between total absolute fecundity and body weight, total length and age were determined 0.59, 0.67 and 0.45, respectively for A. sellal and 0.69, 0.72 and 0.47 respectively, for A. atropatenae. The average total absolute fecundity increased in relation to age, weight and length of females with significant difference (P<0.05). Also, the mean egg diameter of large batches in the ovaries of readily spawning specimens was determined 0 1.35±0.17 and 1.04±0.06 mm for A. sellal and A. atropatenae, respectively. In conclusion, it can be said that these two fish species are batch spawners and spawn 2-3 times during the breeding season. Alburnus sellal and A. atropatenae belong to Leuciscidae family, and are found in southwestern and Urmia Lake basins of Iran, respectively. These fish have ecological and sport fishing values as well. The main purpose of this study was to investigate the relationship between the fecundity with length, weight and age in Gamasiab, Ghaleh-Chai and Mahabad-Chai Rivers. For this purpose, fish sampling was performed in 2008 and 2011 using electrofishing device and Cast-net. The results of the investigation on 47 spicemens of female A. sellal and 37 spicemens of A. atropatenae showed the total absolute fecundity (sum of large, medium and small eggs) in the A. sellal, is 1160-13021 (5571.7±3037.3, average±S.D) and for A. atropatenae, 1205-6588 (3113.2±1252). The total relative fecundity was estimated 125-507 (263.3±86.1) in A. sellal and 144-297 (197.8±41.1) in A. atropatenae per gram of body weight. The correlation (r2) between total absolute fecundity and body weight, total length and age were determined 0.59, 0.67 and 0.45, respectively for A. sellal and 0.69, 0.72 and 0.47 respectively, for A. atropatenae. The average total absolute fecundity increased in relation to age, weight and length of females with significant difference (P<0.05). Also, the mean egg diameter of large batches in the ovaries of readily spawning specimens was determined 0 1.35±0.17 and 1.04±0.06 mm for A. sellal and A. atropatenae, respectively. In conclusion, it can be said that these two fish species are batch spawners and spawn 2-3 times during the breeding season.

https://jfisheries.ut.ac.ir/article_105976_015ec241e2eac531e6c31f8fcba6fb08.pdf