مطالعة تغییر برخی شاخص‌های یونی، بیوشیمیایی و هورمونی در بچه‌ماهیان سفید (Rutilus frisii kutum Kamenskii 1901) رهاسازی‌شده در مصب رودخانة تجن (ساری)

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری، گروه شیلات، دانشکدة منابع طبیعی، دانشگاه تهران، ایران

2 استاد، گروه شیلات، دانشکدة منابع طبیعی، دانشگاه تهران، کرج، ایران

3 دانشیار، گروه شیلات، دانشکدة منابع طبیعی، دانشگاه تهران، ایران

4 استادیار، مؤسسة تحقیقات شیلات ایران، پژوهشکدة اکولوژی آبزیان دریای خزر، ساری، ایران

5 استادیار، عضو هیئت علمی انیستیتو تحقیقاتی بیوفیزیک و بیوشیمی، دانشگاه تهران، ایران

چکیده

 





چکیده






رهاسازی بچه‌ماهیان سفید در نزدیکی مصب رودخانه انجام می‌شود که محل انباشت آلودگی‌های شیمیایی، سموم و فلزات سنگین است. این تحقیق با هدف بررسی چگونگی تطبیق اسمزی و یونی در بچه‌ماهیان سفید و در مصب رودخانة تجن (ساری) با مطالعة برخی شاخص‌های تنظیم فشار اسمزی انجام گرفت. تعداد 50 عدد بچه‌ماهی سفید (5/0 گرم) در هشت قفس رهاسازی شدند که به طور عرضی در محل رهاسازی بچه‌ماهیان سفید در رودخانة مذکور قرار گرفته بودند. قفس‌ها به دو گروه تقسیم شدند: یک گروه روز اول ماهی‌دار و پس از هفت روز نمونه‌برداری شد. گروه دوم در روز چهارم ماهی‌دار شد و نمونه‌برداری از آن‌ها در ساعات 36، 48 و 72 پس از رهاسازی انجام شد. نتایج اختلاف معنا‌داری را از نظر فاکتورهای مورد بررسی (به جز یون کلر و هورمون T4) بین گروه کنترل و گروه‌های آزمایشی نشان داد (05/0 P<). میزان یون‌های بررسی‌شده در گروه‌های آزمایشی بیشتر از گروه کنترل بود و در مورد یون‌های سدیم و پتاسیم روند افزایشی در میزان یون‌ها از ساعت 72 تا روز هفتم دیده شد. میزان هورمون T3 و درصد رطوبت بدن در ماهیان نگهداری‌شده در قفس‌ها کمتر از گروه کنترل بود و در برخی موارد اختلاف معنا‌داری با گروه کنترل داشت (05/0 P<). مقدار هورمون T4، گلوکز، پروتئین کل و درصد تلفات در ماهیان نگهداری‌شده در قفس‌ها به طور غالب بیش از گروه کنترل بود. از نظر هورمون کورتیزول میزان و وضعیت نسبتاً مشابهی بین گروه قفس‌ها و گروه کنترل تا ساعت 72 دیده شد، اما در روز هفتم روند کورتیزول در گروه کنترل افزایشی و در گروه قفس‌ها کاهشی ارزیابی شد. برایند نتایج حاکی از اختلال در موفقیت بچه‌ماهیان سفید در تنظیم اسمزی و تطبیق با آب شور در رودخانة تجن است.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

The change of some ion, hormone and biochemical factors in released fingerling of kutum fish (Rutilus frisii kutum Kamensky, 1901) at the estuarine of Tajan river (Sari)

نویسندگان [English]

  • Mohammad Mohiseni 1
  • Bagher Mojazi Amiri 2
  • Alireza Mirvaghefi 3
  • Mohammad Vahid Farabi 4
  • Gholamhossein Riazi 5
1 Ph.D Candidate of Fisheries, Faculty of Natural Resources, University of Tehran, Karaj, Iran
2 Professor, Department of Fisheries, University of Tehran, Karadj, Iran
3 Associate Professor, Department of Fisheries, Faculty of Natural Resources, University of Tehran, Karaj, Iran
4 Assistant Professor, Iranian Fisheries Research Organization, Ecology Faculty of the Caspian Sea, Sari, Iran
5 Assistant Professor, Institute of Biochemistry and Biophysics, University of Tehran, Iran
چکیده [English]

Releasing of Kutum fish (Rutilus frisii kutum Kamenskii 1901) fingerling almost done in the estuarine,
where are cumulative site of various pollutant such as chemicals, pesticides and heavy metals.
Therefore, investigation of the modality of osmo&ion regulation of kutum fingerlings in the Tajan
river (Sari) by means of some osmoregulatory factors, was the aim of this study. Fifty fingerling fishes
(0.5 gram) were released in eight cages that located in transverse manner in the estuarine of mentioned
river. Cages were divided to two groups: The first group was stocked at initial time and sampled after
7 days. In order to assessment of osmoregulatory capability of fingerlings in the shorter times, the
second group was stocked at the fourth day of the first group stocking and were sampled after 36, 48
and 72 hours. Based on results, significant difference was observed about obtained factors (except of
chloride and T4) between experimental cages and control groups (P<0.05). An amount of measured
ions in fingerling fishes of experimental cages was greater than control group and ascending trend was
observed in the case of sodium, chloride and potassium from 72 hours to seventh day. The rate of T3
and water body content (%) in the fish of cage group were lower than control group and significant
difference was observed in particular times (P<0.05). The quantity of T4, glucose, total protein and
mortality percent in the fingerling fishes of cage group were dominantly bigger than control group.
The same trends was observed in the case of cortisol between two groups until 72 hours, but an
ascending and descending trend was detected at the seventh day in control and cage groups
respectively. Resultant of the obtained results osmoregulatory disorder and failure in salinity
adaptation in fingerling of the kutum fish was observed.

کلیدواژه‌ها [English]

  • kutum fish fingerling
  • Osmoregulation
  • Pollution
  • salinity adaptation
  • Tajan river

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شیلات
دوره 68، شماره 1 - شماره پیاپی 1
فروردین 1394
صفحه 139-155

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