تنوع ژنتیکی اردک‌ماهی (Linnaeus, 1758; Esox lucius) در شرق دریای خزر با استفاده از نشانگر ریزماهواره

نوع مقاله : مقاله پژوهشی

نویسندگان

1 استاد گروه شیلات دانشکدة منابع طبیعی دانشگاه تربیت مدرس، نور

2 کارشناس ارشد رشتة تکثیر و پرورش آبزیان دانشگاه تربیت مدرس، نور

3 استادیار گروه زیست‌شناسی دریا دانشگاه تربیت مدرس، نور

چکیده

 
اردک‌ماهی یکی از گونه‌های اقتصادی و باارزش حوزة جنوبی دریای خزر است که اطلاعات دربارة جمعیت‌های مختلف آن در حوزة جنوبی خزر در دسترس نیست. با توجه به اهمیت شناخت تنوع ژنتیکی در مدیریت ذخایر و حفاظت از گونه‌ها در این مطالعه از هفت جفت آغازگر ریزماهواه‌ای برای بررسی 90 نمونه اردک‌ماهی از سه منطقة دهانة رودخانة تجن، آب‌بندان‎‌های ایزدشهر و تالاب لپوی زاغمرز واقع در استان مازندران استفاده شد. میانگین تعداد آلل مشاهده‌شده در نمونه‌های رودخانة تجن 286/0 و ایزدشهر 010/1 و تالاب لپوی 800/0 و میانگین هتروزیگوسیتی مشاهده‌شده و مورد انتظار برای مجموع نمونه‌ها به ترتیب 383/0 و 621/0 محاسبه شد. نتایج آزمون انحراف از تعادل هاردی- واینبرگ نشان داد که تنها یکی از نمونه‌ها در تعادل قرار داشت. با توجه به نتایج، علائمی از تنگنای ژنتیکی در جمعیت‌ها دیده شد. دندروگرام ترسیمی و آزمون واریانس مولکولی بیانگر وجود سه جمعیت مجزا در سه منطقة مورد مطالعه بود که می‌بایست در مدیریت ذخایر این گونه مورد توجه قرار گیرد.
 

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

The genetic structure of Pike Esox lucius Linnaeus 1758 in the Southeast of the Caspian Sea, using microsatellite markers

نویسندگان [English]

  • Mohammad Reza Kalbassi 1
  • mona tabarrok 2
  • Mohammad Sadegh Alavi Yeganeh 3
1 Prof. Dept. of Fisheries, Tarbiat Modaress University, Mazandaran, Nour, Iran
2 (M.Sc.) of Fisheries, Tarbiat Modaress University ,Mazandaran, Nour, Iran.
3 Assistant Prof.Dep. Marine Science, Tarbiat Modaress University ,Mazandaran, Nour, Iran.
چکیده [English]

Esox lucius Linnaeus1758  is one of the economically valuable species of Caspian Sea. Genetic diversity of marine resources is of vital importance in their management and protection. Considering the lack of information about genetic differences this species; seven microsatellite loci were used for 90 sample analyses. The results showed conspicuous genetic variation in regions using Fst, AMOVA and a relatively high level of gene flow was found among populations. The average observed and expected heterozygosities were 0.383 and 0.622, respectively. Assessment of Hardy-Weinberg equilibrium showed that all samples of  studied tests were significantly deviated from H-W equilibrium (p ≤ 0.05). Results show evidences for genetic bottleneck in the populations. Graphical cluster represents a distinct molecular variance test, there are three distinct and separate population in the three areas that should be considered in the management of these reserves.
 
 

کلیدواژه‌ها [English]

  • Esox lucius
  • Microsatellite
  • heterozygosity
  • Hardy-Weinberg equilibrium

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