ارزیابی شاخص‌های رشد، ایمنی و هماتولوژی سرم خون در جیره بچه ماهیان قزل‌آلای رنگین‌کمان (Oncorhynchus mykiss) مبتنی بر پودر ملخ مهاجر (Locusta migratoria)

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانش‌آموخته دکتری گروه شیلات، دانشکده منابع طبیعی، دانشگاه گیلان، صومعه سرا، گیلان، ایران

2 دانشیار گروه تکثیر و پرورش، دانشکده شیلات و محیط زیست، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران

3 دانشیار گروه شیلات، دانشکده منابع طبیعی، دانشگاه گیلان، صومعه سرا، گیلان، ایران

4 کارشناس محیط زیست، اداره محیط زیست تهران، تهران، ایران

10.22059/jfisheries.2022.347446.1341

چکیده

در این مطالعه بررسی امکان استفاده از ملخ مهاجر به عنوان منبع تامین کننده پروتئین در جیره بچه ماهیان قزل‌آلای رنگین‌کمان و تاثیر آن بر عملکرد رشد، ایمنی و هماتولوژی سرم خون مورد بررسی قرار گرفت. تعداد 300 قطعه ماهی قزل‌آلا با میانگین وزنی (22/0± 09/6 گرم) با جیره‌های غذایی 0 درصد (شاهد)، 15 درصد (LM15)، 30 درصد (LM30) و 60 درصد (LM60) پودر ملخ فرموله شدند. هر جیره در قالب طرح کاملا تصادفی در 3 تکرار و با 30 قطعه ماهی با ایجاد شرایط یکسان، در هر مخزن فایبرگلاس توزیع شدند. در پایان آزمایش، شاخص­های رشد، ایمنی و هماتولوژی سرم خون بر اساس فرمول‌های استاندارد محاسبه وآنالیز با استفاده آزمون توکی انجام شد. نتایج نشان داد بیشترین وزن نهایی در تیمار 30 درصد مشاهده شد (89/34 ± 20/616 درصد) و تفاوت بین تیمارهای صفر، 15و 60 درصد معنی­دار بود (05/0> p). در تیمار 60 درصد افزایش معنی­دار ضریب تبدیل غذایی نسبت به تیمار شاهد مشاهده شد (05/0 > p). در تغذیه ماهیان با جیره حاوی پودر ملخ، مقادیرگلوکز، آلبومین، گلوبین و IgM اختلاف معنی داری را در بین تیمارهای مختلف آزمایشی نشان ندادند (05/0> p). اما میزان پروتئین کل، تری­گلیسرید و کلسترول خون، اختلاف معنی دار در تیمار‌های ازمایشی نسبت به تیمار شاهد نشان داد (05/0 > p). بنابراین می­توان نتیجه گرفت استفاده از 30 درصد پودر ملخ در جیره تجاری بچه ماهیان قزل‌آلا می­تواند باعث بهبود عملکرد رشد شده و فاقد تاثیر منفی بر سطح مطلوب شاخص‌های خونی و ایمنی گردد.

کلیدواژه‌ها

عنوان مقاله [English]

Evaluation of growth, immunity and blood serum hematology indicators in the diet of rainbow trout fingerling (Oncorhynchus mykiss) based on locust meal (Locusta migratoria)

نویسندگان [English]

  • hadi Asadi 1
  • majidreza khoshkholgh 2
  • hamid noverian 3
  • Roghayyeh Safari 2
  • Abbas gheytasi 4
1 Graduated student, Fisheries Department, Faculty of Natural Resources, University of Guilan, Sowmeh Sara, Guilan, Iran
2 Associate Professor, Fisheries Group, Department of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
3 Associate Professor, Fisheries Group, Department of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
4 Environmental expert, Tehran Environment Department, Tehran, Iran
چکیده [English]

In this study, the possibility of using migratory locust as a source of protein in the diet of rainbow trout fry and its effect on growth performance, immunity and hematology of blood serum was investigated. 300 number of rainbow trout fingerling with an average weight (6.09 ± 0.22 gr) with diets of 0% (control), 15% (LM15), 30% (LM30) and 60% (LM60) of formulated locust meal. became Each diet was distributed in a completely random design in 3 replicates with 30 number of fish in each fiberglass tank with the same conditions. At the end of the experiment, the indicators of growth, immunity and blood serum hematology were calculated based on standard formulas and analyzed using Tukey's test. The results showed that the highest final weight was observed in the 30% treatment (616.20% ± 34.89%) and the difference between zero, 15% and 60% treatments was significant (p<0.05). In the 60% treatment, a significant increase in feed conversion ratio was observed compared to the control treatment (p < 0.05). In feeding fish with a diet containing locust meal, the values of glucose, albumin, globin and IgM did not show significant differences among different experimental treatments (p<0.05). However, the amount of total protein, triglyceride and blood cholesterol showed a significant difference in the experimental treatments compared to the control treatment (p < 0.05). Therefore, it can be concluded that the use of 30% of locust meal in the commercial diet of rainbow trout fingerling can improve the growth performance and have no negative effect on the desired level of blood and immune parameters.
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کلیدواژه‌ها [English]

  • Insect protein
  • Replacement
  • Blood and Nutritional Indicators
  • Rainbow trout

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