بررسی تأثیر سینامالدهید روی عوارض ناشی از اکسیدروی (ZnO) در آبشش ماهی کپور معمولی (Cyprinus carpio)

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانش‌آموخته دکتری دامپزشکی، دانشگاه آزاد واحد بابل، بابل، ایران

2 استادیار گروه پاتولوژی، دانشکده دامپزشکی، دانشگاه آزاد واحد بابل، بابل، ایران

3 استادیار گروه زیست دریا، دانشکده علوم دریایی و محیطی، دانشگاه مازندران، بابلسر، ایران

10.22059/jfisheries.2023.353163.1358

چکیده

امروزه استفاده از گیاهان به‌عنوان یکی از راه ­های درمانی آلودگی با فلزات سنگین بسیار مورد توجه قرار گرفته است. دارچین از جمله گیاهانی است که دارای خواص درمانی بسیاری است که این خواص به علت مواد مؤثر موجود در آن است. یکی از مهمترین مواد مؤثر دارچین سینامالدهید است. در مطالعة حاضر اثر درمانی سینامالدهید بر ضایعات ناشی از اکسید روی (ZnO) در آبشش ماهی کپور معمولی (Cyprinus carpio) مورد بررسی قرار گرفت. ماهی‌های کپور معمولی در 5 گروه با سه تکرار تقسیم شدند. گروه 1 به‌عنوان شاهد در نظر گرفته شد، گروه 2 در معرض 3 میلی‌گرم در لیتر اکسید روی قرار گرفت و گروه‌های 3، 4 و 5 علاوه بر دریافت اکسید روی به‌ترتیب غلظت‌های 3/6، 7/2 و 10/8 میلی‌گرم سینامالدهید را در 100 گرم غذا به‌صورت خوراکی به‌مدت 45 روز دریافت کردند. سپس از هر گروه 6 ماهی به‌طور تصادفی جدا و از آبشش ماهی‌ها نمونه‌‌برداری شد. در این مطالعه بیشترین میزان غلظت روی، کمترین مقدار فعالیت کل آنتی‌اکسیدانی و بیشترین مقدار شاخص پراکسیداسیون لیپید در گروه 2 مشاهده شد. همچنین شدیدترین آسیب در گروه 2 به‌صورت هیپرپلازی سلول‌های بافت پوششی، اتصال، آتروفی و تخریب تیغه‌های آبششی ثانویه، پرخونی، تلنژکتازی و نفوذ سلول‌های آماسی مشاهده گردید. در گروه‌های درمانی با افزایش میزان دریافت سینامالدهید غلظت روی، علایم آسیب‌شناسی بافت و مقدار شاخص پراکسیداسیون لیپید کاهش و مقدار فعالیت کل آنتی‌اکسیدانی افزایش پیدا کردند. نتایج حاصل از این مطالعه نشان داد سینامالدهید در مقابل عوارض ناشی از اکسید روی تأثیر حفاظتی و درمانی دارد. بیشترین اثر حفاظتی در گروه 5 به‌صورت کاهش تجمع زیستی روی، بهبود علایم آسیب‌شناسی بافتی ناشی از روی، کاهش مقدار شاخص پراکسیداسیون لیپید و افزایش مقدار فعالیت کل آنتی‌اکسیدانی مشاهده گردید.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

The effect of cinnamaldehyde on the lesions caused by zinc oxide (ZnO) in the gills of common carp (Cyprinus carpio)

نویسندگان [English]

  • Farzad Heidardokht 1
  • Seyed Mohammad Hosseini 2
  • Shila Omidzahir 3
1 Graduated student, Faculty of Veterinary Medicine, Islamic Azad University, Babol branch, Babol, Iran
2 Assistant Professor, Department of Pathology, Faculty of Veterinary Medicine, Islamic Azad University, Babol branch, Babol, Iran
3 Assistant Professor, Faculty of Marine and environmental Sciences, University of Mazandaran, Babolsar, Iran
چکیده [English]

Nowadays, the use of plants as one of the treatment methods of heavy metal pollution is considered. Cinnamon is one of the plants that has many therapeutic properties due to its effective ingredients. One of the most important effective ingredients of cinnamon is cinnamaldehyde. In the current study, the therapeutic effect of cinnamaldehyde on the lesions caused by zinc oxide (ZnO) in the gills of common carp (Cyprinus carpio) was investigated. In this study, common carp were divided into 5 groups in triplicates. Group 1 was considered as a control, group 2 was exposed to 3mg/L of zinc oxide, and groups 3, 4, and 5, in addition to receiving zinc oxide received concentrations of 3.6, 7.2, and 10.8mg of cinnamaldehyde in 100g diet, respectively for a period of 45 days. Then, 6 fishes were randomly selected from each group and their gills were sampled. The highest amount of zinc concentration, the lowest amount of total antioxidant activity and the highest amount of lipid peroxidation index were observed in group 2. Also, the most severe damage was observed in group 2 including epithelial cells hyperplasia, adhesion, atrophy and destruction of secondary lamellae, hyperemia, telangiectasia and infiltration of inflammatory cells. In the treatment groups with increase of cinnamaldehyde in the diets, zinc concentration, histopathology symptoms and lipid peroxidation index decreased, while the amount of total antioxidant activity increased. The results of this study showed that cinnamaldehyde has a protective and therapeutic effect against the damage caused by zinc oxide. The most protective effect was observed in group 5 including decrease of Zn bioaccumulation, improving histopathology symptoms, reducing the value of lipid peroxidation index and increasing the value of total antioxidant activity.

کلیدواژه‌ها [English]

  • Cyprinus carpio
  • Cinnamon
  • Heavy metals
  • Bioaccumulation and Antioxidant

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