تأثیر جیرة غذایی حاوی پودرماهی فاسد و اثرات حفاظتی مکمل ویتامینی E و C بر شاخص‌های رشد، خون‌شناسی و بیوشیمیایی بچه‌ماهیان قزل‌آلای رنگین‌کمان (Oncorhynchus mykiss)

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری گروه شیلات و آبزیان، دانشکده منابع طبیعی، دانشگاه ارومیه، ارومیه، ایران

2 دانشیار گروه شیلات و آبزیان، دانشکده منابع طبیعی، دانشگاه ارومیه، ارومیه، ایران

3 استادیار گروه شیمی تجزیه، کروماتوگرافی جهاد دانشگاهی، واحد ارومیه ، ارومیه، ایران

10.22059/jfisheries.2023.351064.1351

چکیده

پژوهش حاضر با هدف بررسی تأثیر پودر ماهی فاسد به­ همراه اثرات حفاظتی ویتامین ­های E و C بر شاخص‌های رشد، خون‌شناسی و بیوشیمیایی بچه‌ماهیان قزل‌آلای رنگین‌کمان انجام گرفت. بدین‌منظور 240 قطعه بچه‌ماهی با میانگین وزنی 0/25±12/75 گرم در چهار تیمار شامل تیمار 1: جیرة غذایی حاوی پودر ماهی تازه، تیمار 2: پودر ماهی فاسد، تیمار 3: پودر ماهی فاسد به‌همراه سطح کم مکمل ویتامینی (mg/kg 100 میلی گرم/کیلوگرم ویتامین E، 200 میلی گرم/کیلوگرم ویتامین C) و تیمار 4: پودر ماهی فاسد به‌همراه سطح زیاد مکمل ویتامینی (200 میلی گرم/کیلوگرم ویتامین E، 400 میلی گرم/کیلوگرم ویتامین C)، به‌مدت 60 روز تغذیه شدند. در پایان دوره آزمایش، شاخص‌های وزن نهایی، نرخ رشد ویژه، درصد افزایش وزن بدن و ضریب تبدیل غذایی محاسبه شدند. همچنین شاخص‌های خونی، کلسترول، تری‌گلیسیرید، HDL و LDL مورد سنجش قرار گرفتند. نتایج به‌دست آمده نشان داد که شاخص‌های رشد نهایی، نرخ رشد ویژه و درصد افزایش وزن بدن در تیمارهای 2، 3 و 4 کاهش و ضریب تبدیل غذایی افزایش یافت (0/05≥P). تعداد گلبول‌های سفید (WBC) و هموگلوبین (Hb) در تیمارهای 3 و 4 و تعداد گلبول‌های قرمز (RBC) و درصد هماتوکریت (Htc%) در تیمار 3 افزایش یافت (0/05≥P). تری‌گلیسیرید تحت تأثیر مکمل ویتامینی در تیمارهای 3 و 4 کاهش یافت (0/05≥P) ولی کلسترول و LDL در تیمارهای 2 و 3 افزایش و در تیمار 4 کاهش یافت (05/0≥P). HDL نیز در تیمارهای حاوی مکمل ویتامینی 3 و 4 افزایش یافت (0/05≥P). می‌توان چنین نتیجه‌گیری نمود که پودر ماهی فاسد بر شاخص‌های رشد، خون‌شناسی و بیوشیمیایی تأثیر منفی دارد و مکمل ویتامینی در کاهش این اثرات در شاخص‌های خون‌شناسی و بیوشیمیایی مؤثر است.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

The effect of a diet containing spoiled fish meal and the protective effects of vitamin E and C supplements on growth, hematological and biochemical indices of rainbow trout juveniles (Oncorhynchus mykiss)

نویسندگان [English]

  • Mojtaba Pourahad Anzabi 1
  • Kourosh Sarvi Moghanlou 2
  • Ahmad Imani 2
  • Raheleh Tahmasebi 3
1 Ph.D. Student, Department of Fisheries, Faculty of Natural Resources, University of Urmia, Urmia, Iran
2 Associate Professor, Department of Fisheries, Faculty of Natural Resources, University of Urmia, Urmia, Iran
3 Assistant Professor, Department of Chromatography, Iranian Academic Center for Education, Culture and Research, Urmia, Iran
چکیده [English]

The present study was carried out with the aim of investigating the effect of a dietary spoiled fish meal along with the protective effects of vitamins E and C on the growth, hematological and biochemical indices of rainbow trout fingerlings. For this purpose, 240 rainbow trout fingerlings with an average weight of 12.75±0.25g were fed on 4 treatments diets including: 1: a diet containing fresh fish meal; treatment 2: a diet with spoiled fish meal; treatment 3: a diet with spoiled fish meal and low level of vitamin supplements (100mg/kg vitamin E, 200mg/kg vitamin C) and treatment 4: a diet containing spoiled fish meal and high levels of vitamin supplements (200mg/kg vitamin E, 400mg/kg vitamin C) for 60 days. At the end of the test period, the indices of final weight, specific growth rate, body weight gain, and food conversion ratio were calculated. Also, hematological parameters, cholesterol, triglyceride, HDL, and LDL were evaluated. Based on the obtained results, the final weight, specific growth rate, and body weight gain decreased in treatments 2, 3, and 4, while the food conversion ratio increased (P≥0.05). WBC and Hb increased in treatments 3 and 4 (P≥0.05). RBC and Htc% augmented in treatment 3 (P≥0.05). Triglyceride diminished in treatments 3 and 4 (P≥0.05), but cholesterol and LDL increased in treatments 2 and 3 and decreased in treatments 2 (P≥0.05). HDL has grown in treatments 3 and 4 (P≥0.05). It can be concluded that spoiled fish meal harms growth, hematological, and biochemical indices, and vitamin supplements are impacts on reducing these effects in hematological and biochemical indices.

کلیدواژه‌ها [English]

  • Spoiled fish meal
  • Vitamin E
  • Vitamin C
  • Growth indices
  • hematological and biochemical parameters
  • Rainbow trout

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