تغییرات فصلی میزان آگار و دو هورمون گیاهی در ماکرو جلبک Gracilaria corticata (Rhodophyta)

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانش‌آموخته دکتری، گروه فناوری‌های زیستی، دانشکدة منابع طبیعی و محیط‌زیست، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران

2 استادیار گروه بیولوژی دریا، واحد تهران شمال، دانشگاه آزاد اسلامی، تهران، ایران

3 استادیار گروه شیمی، واحد علوم و تحقیقات تهران، دانشگاه آزاد اسلامی، تهران، ایران

4 استادیار،گروه زیست‌شناسی، واحد یادگار امام خمینی (ره) شهرری، دانشگاه آزاد اسلامی، تهران، ایران

10.22059/jfisheries.2024.367216.1408

چکیده

جلبک‌های دریایی قرمز حاوی ترکیباتی مانند آگار و هورمون‌های تنظیم‌کنندة رشد هستند که در صنایع مصارف متعددی دارند. این تحقیق جهت تعیین تودة ‌زندة و استخراج دو هورمون و آگار از جلبک Gracilaria corticata انجام شد. نمونه‌برداری از دی 1394 تا آبان 1395 در بندر بوشهر یک ماه در میان انجام گرفت. تودة ‌زنده و پارامترهای محیطی در ترانسکت انتخابی اندازه‌گیری و جلبک برداشت شد. پس از مراحل استخراج، با روشHPLC هورمون‌ها جداسازی و توسط تزریق استاندارد شناسایی شدند. بیشترین میزان آگار در تیر ماه 63/3 % ، زآتین در شهریور ماه 21/90% و ایندول بوتیریک اسید 21/19% در دی‌ماه بود. بیشترین تودة زندة این جلبک در اسفند ماه 423/33 گرم در مترمربع اندازه‌گیری شد. نتایج حاصل از آزمون آماری ANOVA وChi-squared تفاوت معنی‌داری را در 6 ماه نمونه‌برداری در تمامی نمونه‌ها نشان داد (0/05>P). در بین عوامل محیطی آگار با شوری همبستگی داشت. ایندول بوتیریک اسید در دی ماه باعث رشد زیاد جلبک شد اما زآتین تأثیر معنی‌داری بر رشد این گونه نشان نداد. به‌طور کلی می‌توان به‌منظور استخراج این مواد، در ماه‌هایی که بیشترین مقدار را داشته‌اند برداشت صورت گیرد. با توجه به قیمت گزاف این هورمون‌ها و ضرورت استفاده از آن‌ها در عصاره‌های جلبکی و کودمایع جلبکی، این تحقیق شروعی برای استخراج انواع هورمون‌ها از این جلبک‌ها است.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Seasonal variation of agar and two plant hormones in Macroalgae Gracilaria corticata (Rhodophyta)

نویسندگان [English]

  • Hadigheh Saebmehr 1
  • Farnaz Rafiee 2
  • Mohammad Hadi Givianrad 3
  • Golaleh Mostafavi 4
1 Ph.D graduate, Department of Natural Resources & Environment, Science & Research Branch, Islamic Azad University, Tehran, Iran
2 Assistant Professor, Department of Marine Biology, North Tehran Branch, Islamic Azad University, Tehran, Iran
3 Assistant Professor, Department of Chemistry, Science & Research Branch, Islamic Azad University, Tehran, Iran
4 Assistant Professor,Department of Biology,Yadegar-e-Imam Khomeini (RAH) Shahr-e- Rey Branch, Islamic Azad University, Tehran, Iran
چکیده [English]

Red seaweeds contain compounds such as agar and hormones that have many uses in industry. This research was done to determine biomass and extract two hormones and agar from Gracilaria corticata algae. Methods Sampling was done from December 2014 to November 2015 in Bushehr port, one month apart. Biomass and environmental parameters were measured and algae harvested in the selected transect. After the extraction steps, hormones are separated by the HPLC method and identified by standard injection. The highest amount of agar occurred in July with 63.3%, Zeatin in September with 21.9% and indole butyric acid with 19.21% in January. The highest fresh weight of this algae was measured in March at 423.33 grams per square meter. The results of the ANOVA and Chi-squared statistical tests showed a significant difference in 6 months of sampling in all samples (P<0.05). The amount of agar showed a significant correlation with the biomass. Also, among environmental factors, agar was correlated with salinity and temperature. Butyric indole of acid in January caused the growth of alga, but Zeatin did not show a significant effect on the growth of this species. In general, in order to extract these materials, harvesting can be done in the months that have the highest amount. Considering the exorbitant price of these hormones and the need to use them in algae extracts and algae liquid fertilizer, this research is a starting point for extracting all kinds of hormones from these algae.

کلیدواژه‌ها [English]

  • Agar
  • Extraction
  • Gracilaria
  • Growth regulator
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