بررسی شاخص های رشد و تغذیه، آسیب شناسی بافت روده و خون شناسی بچه ماهی قزل‌آلای رنگین کمان (Oncorhynchus mykiss) تغذیه شده با سطوح مختلف عصارة هیدروالکلی گیاه مرزه (Satureja hortensis)

نوع مقاله : مقاله پژوهشی

نویسندگان

1 کارشناسی ارشد گروه شیلات، دانشکدة شیلات و محیطزیست، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران

2 استاد گروه شیلات، دانشکدة شیلات و محیط‌زیست، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران

3 دانش آموختة دکتری گروه شیلات، دانشکدة شیلات و محیط‌زیست، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران

4 دانش آموختة دکتری گروه شیلات، دانشکدة علوم و فنون دریایی، دانشگاه هرمزگان، بندرعباس، ایران

10.22059/jfisheries.2024.374222.1421

چکیده

استفاده از عصاره‌های گیاهی به‌عنوان افزودنی تغذیه‌ای در صنعت آبزی‌پروری، باعث افزایش مقاومت آبزیان در برابر عوامل عفونی می‌شود و در عین حال به‌عنوان جایگزین آنتی‌بیوتیک‌های سنتی، سیستم ایمنی و رشد ماهیان را تحت پوشش قرار می‌دهد. مطالعه حاضر برای بررسی اثرات رژیم غذایی حاوی عصارة هیدروالکلی گیاه مرزه (Satureja hortensis) بر عملکرد رشد، پارمتر‌های خونی و آسیب‌شناسی بافت روده بچه‌ماهی قزل­آلای رنگین‌کمان (Oncorhynchus mykiss) طراحی گردید. بدین‌منظور، 240 قطعه بچه ماهی با میانگین وزن 0/02±2/02 گرم به‌صورت یک طرح کاملا تصادفی در 4 گروه آزمایشی با سه تکرار به‌مدت 6 هفته با جیره­ هایی شامل یک گروه شاهد و سه تیمار حاوی غلظت­ های 0/1 (تیمار یک)، 0/5 (تیمار دو) و 1 (تیمار سه) درصد عصارة هیدروالکلی گیاه مرزه تغذیه شدند. نتایج نشان داد که شاخص­ های عملکرد رشد شامل وزن نهایی و افزایش وزن بدن و ضریب رشد ویژه در تیمار تغذیه شده با جیرة حاوی 1 درصد عصاره به‌طور معنی­ داری در مقایسه با گروه شاهد افزایش یافت. همچنین، تعداد سلول‌های سفید و قرمز خون و شاخص­های خونی مانند میزان هماتوکریت در تیمارهای تغذیه شده با عصارة مرزه افزایش یافت (0/05>P). استفاده از عصارة هیدروالکلی گیاه مرزه در جیرة غذایی بچه‌ماهیان قزل ­آلای رنگین‌کمان در هیچ یک از گروه­ ها تأثیرات منفی مانند واکنش ­های التهابی یا تخریبی بر قسمت انتهایی بافت رودة بچه ماهیان نداشت. در مجموع، نتایج نشان داد که تغذیه با یک درصد عصارة هیدروالکلی مرزه در جیرة غذایی می‌تواند به بهبود سلامت و رشد بهتر بچه‌ماهی قزل‌آلای رنگین‌کمان کمک کند.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Investigation of growth and nutritional indices, histopathology of the intestine, and hematological parameters of rainbow trout (Oncorhynchus mykiss) fry fed with different levels of hydroalcoholic extract of Savory (Satureja hortensis)

نویسندگان [English]

  • Mohammad Mirzaee 1
  • Abdolmajid Hajimoradloo 2
  • Ali Jafa Nodeh 3
  • Marzieh Abolfathi 4
1 M.Sc. student, Department of Fisheries, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
2 Professor, Department of Fisheries s, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
3 PhD graduate, Department of Fisheries, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
4 PhD graduate, Department of Fisheries, Faculty of marine science and technology, university of Hormozgan, Bandar Abbas, Iran
چکیده [English]

Using herbal extracts as nutritional additives in aquaculture industry increases the resistance of fish to infectious agents while also providing a substitute for traditional antibiotics, enhancing the immune system, and promoting the growth of fish. The present experiments were designed to investigate the effects of a diet containing hydroalcoholic extract of savory (Satureja hortensis) on the growth performance, hematological parameters, and histopathology of the intestine in rainbow trout (Oncorhynchus mykiss) fry. For this purpose, 240 rainbow trout fry with an average weight of 2.02 ± 0.02 grs were randomly distributed into 4 experimental groups with three replicates and fed diets containing concentrations of 0 (control), 0.1%, 0.5%, and 1% hydroalcoholic extract of savory for 6 weeks. The results showed that growth performance indices including final weight, body weight gain, and specific growth rate significantly increased in the group fed with a diet containing 1% extract compared to the control group. Additionally, the number of white and red blood cells, and hematocrit levels increased in the groups fed with savory extract (P<0.05). The use of hydroalcoholic extract of savory in the diet of rainbow trout fry had no negative effect on the distal intestine tissue of the fish in all groups. Overall, the results indicated that feeding with 1% hydroalcoholic extract of savory in the diet could improve the health and better growth of rainbow trout fry.

کلیدواژه‌ها [English]

  • Satureja hortensis
  • Rainbow trout
  • Growth performance
  • Hematological parameters
  • Intestine tissue
Cahu, C.L., Gisbert, E., Villeneuve, L.A., Morais, S., Hamza, N., Wold, P.A., Zambonino Infante, J.L., 2009. Influence of dietary phospholipids on early ontogenesis of fish. Aquaculture Research 40(9), 989-999. DOI: 10.1111/j.1365-2109.2009.02190.x
Camargo, M. M., Martinez, C.B., 2007. Histopathology of gills, kidney and liver of a Neotropical fish caged in an urban stream. Neotropical Ichthyology 5, 327-336. DOI: 10.1590/S1679-62252007000300013
Darvishi, M., Shamsaie Mehrgan, M., Khajehrahimi, A.E., 2022. Effect of licorice (Glycyrrhiza glabra) extract as an immunostimulant on serum and skin mucus immune parameters, transcriptomic responses of immune-related gene, and disease resistance against Yersinia ruckeri in rainbow trout (Oncorhynchus mykiss). Frontiers in Veterinary Science 9, 811684. DOI: 10.3389/fvets.2022.811684
Fierascu, I., Dinu-Pirvu, C.E., Fierascu, R.C., Velescu, B.S., Anuta, V., Ortan, A., Jinga, V., 2018. Phytochemical profile and biological activities of Satureja hortensis L.: A review of the last decade. Molecules 23(10), 2458. DOI: 10.3390/molecules23102458
Fynn-Aikins, K., Hung, S. S. O., Liu, W., Li, H., 1992. Growth, lipogenesis and liver composition of juvenile white sturgeon fed different levels of D-glucose. Aquaculture 105(1), 61-72. DOI: h10.1016/0044-8486(92)90162-E
Ghafarifarsani, H., Hoseinifar, S.H., Aftabgard, M., Van Doan, H., 2022. The improving role of savory (Satureja hortensis) essential oil for Caspian roach (Rutilus caspicus) fry: Growth, haematological, immunological, and antioxidant parameters and resistance to salinity stress. Aquaculture 548, 737653. DOI:10.1016/j.aquaculture.2021.737653
Ghafarifarsani, H., Rashidian, G., Sheikhlar, A., Naderi Farsani, M., Hoseinifar, S. H., Van Doan, H., 2021. The use of dietary oak acorn extract to improve haematological parameters, mucosal and serum immunity, skin mucus bactericidal activity, and disease resistance in rainbow trout (Oncorhynchus mykiss). Aquaculture Research 52(6), 2518-2527. DOI: 10.1111/are.15101
Hajhashemi, V., Zolfaghari, B., Yousefi, A., 2012. Antinociceptive and anti-inflammatory activities of Satureja hortensis seed essential oil, hydroalcoholic and polyphenolic extracts in animal models. Medical Principles and Practice 21(2), 178-182. DOI: 10.1159/000333555
Harikrishnan, R., Balasundaram, C., Heo, M.-S., 2011. Impact of plant products on innate and adaptive immune system of cultured finfish and shellfish. Aquaculture 317(1), 1-15. DOI:10.1016/j.aquaculture.2011.03.039
Jalil, A.T., Abdelbasset, W.K., Shichiyakh, R.A., Widjaja, G., Altimari, U.S., Aravindhan, S., Thijail, H.A., Mustafa, Y.F., Naserabad, S., 2022. Protective effect of summer savory (Satureja hortensis) essential oil on some growth, biochemical, immune serum, mucosal immune system and antioxidant parameters of common carp (Cyprinus carpio) exposed to pretilachlor herbicide. DOI: 10.21203/rs.3.rs-1198387/v1
Karimi, N., Yari, M., Ghasmpour, H.R., 2012. Identification and comparison of essential oil composition and mineral changes in different phenological stages of Satureja hortensis L. Iranian Journal of Plant Physiology 3(1), 577-582. (In Persian)
Kudełka, W., Kosowska, A., 2008. Components of spices and herbs determining their functional properties and their role in human nutrition and prevention of diseases. Cracow Review of Economics and Management 78, 83-111.
Mai, K., Yu, H., Ma, H., Duan, Q., Gisbert, E., Infante, J. Z., Cahu, C. L., 2005. A histological study on the development of the digestive system of Pseudosciaena crocea larvae and juveniles. Journal of Fish Biology 67(4), 1094-1106. DOI: 10.1111/j.0022-1112.2005.00812.x
Mehrabi, Z., Firouzbakhsh, F., Rahimi-Mianji, G., Paknejad, H., 2020. Immunity and growth improvement of rainbow trout (Oncorhynchus mykiss) fed dietary nettle (Urtica dioica) against experimental challenge with Saprolegnia parasitica. Fish & Shellfish Immunology 104, 74-82. DOI: 10.1016/j.fsi.2020.05.050
Mirzaee, M., Safari, R., 2023. Stress in ornamental fish and the use of medicinal plants for its control. Ornamental Aquatic 10(3). 47-31. (In Persian) DOI: 20.1001.1.24234575.1402.10.3.4.6
Mohammadalikhani, M., Shamsaei Mehrjan, M., Haghighi, M., Soltani, M., Kamali, A., 2020. Effects of dietary purslane (Portulaca oleracea) extract on growth performance, hematological indices and immune responses of rainbow trout (Oncorhynchus mykiss) fry. Iranian Journal of Fisheries Sciences 19(5), 2660-2672. DOI: 10.22092/ijfs.2020.122576
Mousavi, E., Mohammadiazarm, H., Mousavi, S. M., Ghatrami, E. R., 2016. Effects of inulin, savory and onion powders in diet of juveniles carp Cyprinus carpio (Linnaeus 1758) on gut micro flora, immune response and blood biochemical parameters. Turkish Journal of Fisheries and Aquatic Sciences 16(4), 831-838. DOI: 10.4194/1303-2712-v16_4_09
Mozafarian, V. 2006. Culture Iran Names of Plants. In: The Contemporary Culture Press, Iran, Tehran.
Mumivand, H., Babalar, M., Hadian, J., Tabatabaei, S. M. F., 2010. Influence of nitrogen and calcium carbonate application rates on the minerals content of summer savory (Satureja hortensis L.) leaves. Horticulture Environment and Biotechnology 51(3), 173-177.
Naigaga, I., Adeyemo, O. K., Alli, R. A., 2009. Effect of handling and transportation on haematology of african catfish (Clarias gariepinus). Journal of Fisheries Sciences 3(4), 333-341.
RamezaniFard, E., Kamarudin, M., Harmin, S., Saad, C.R., Abd Satar, M., Daud, S.K., 2011. Ontogenic development of the mouth and digestive tract in larval Malaysian mahseer, Tor tambroides Bleeker. Journal of Applied Ichthyology 27(3), 920-927. DOI: 10.1111/j.1439-0426.2010.01598.x
Rašković, B., Stanković, M., Marković, Z., Poleksić, V., 2011. Histological methods in the assessment of different feed effects on liver and intestine of fish. Journal of Agricultural Sciences (Belgrade) 56(1), 87-100. DOI: 10.2298/JAS1101087R
Ravindran, P., Pillai, G., Divakaran, M. 2012. Other herbs and spices: mango ginger to wasabi. In Handbook of herbs and spices: Elsevier. DOI: 10.1533/9780857095688.557
Řehulka, J., 2000. Influence of astaxanthin on growth rate, condition, and some blood indices of rainbow trout, Oncorhynchus mykiss. Aquaculture 190(1), 27-47. DOI: 10.1016/S0044-8486(00)00383-5
Reverter, M., Bontemps, N., Lecchini, D., Banaigs, B., Sasal, P., 2014. Use of plant extracts in fish aquaculture as an alternative to chemotherapy: Current status and future perspectives. Aquaculture 433, 50-61. DOI: 10.1016/j.aquaculture.2014.05.048
Roosta, Z., Hoseinifar, S. H., 2016. The effects of crowding stress on some epidermal mucus immune parameters, growth performance and survival rate of tiger barb (Pentius tetrazona). Aquaculture Research 47(5), 1682-1686.
Sadeghi, A. A., Mohamadi-Saei, M., Ahmadvand, H., 2014. The efficacy of dietary savory essential oil on reducing the toxicity of Aflatoxin B1 in broiler chicks. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 20(4), 481.
Soltani, M., Zarifmanesh, T., Zorriehzahra, S. J., 2013. Effect of Zataria multiflora essential oils on rainbow trout (Oncorhynchus mykis) complement component activity and lysozyme. Iranian Scientific Fisheries Journal 21(4), 13-23. DOI:10.22092/isfj.2017.110083
Tepić, A., Zeković, Z., Kravić, S., Mandić, A., 2009. Pigment content and fatty acid composition of paprika oleoresins obtained by conventional and supercritical carbon dioxide extraction. CyTA–Journal of Food 7(2), 95-102.DOI: 10.1080/19476330902940382
Webster, C. D., Lim, C. 2002. Nutrient requirements and feeding of finfish for aquaculture: CABI publishing.
Zeppenfeld, C., Hernández, D., Santinón, J., Heinzmann, B., Da Cunha, M., Schmidt, D., Baldisserotto, B., 2016. Essential oil of A loysia triphylla as feed additive promotes growth of silver catfish (Rhamdia quelen). Aquaculture Nutrition 22(4), 933-940. DOI: 10.1111/anu.12311
Zhou, Q. C., Tan, B. P., Mai, K. S., Liu, Y. J., 2004. Apparent digestibility of selected feed ingredients for juvenile cobia Rachycentron canadum. Aquaculture 241(1), 441-451. DOI: /10.1016/j.aquaculture.2004.08.044