Document Type : Research Paper
Authors
1
MSc., Department of Fisheries, Faculty of Natural Resources, University of Tehran, Karaj, Iran
2
Associate Prof., Department of Biotechnology, Razi Vaccine & Serum Research Institute, Karaj, Iran
3
Assistant Prof., Department of Fisheries, Faculty of Natural Resources, University of Tehran, Karaj, Iran
4
Assistant Prof., Department of Fisheries, Faculty of Natural Resources and Environment, University of Malayer. Malayer, Iran
5
MSc, Department of Biotechnology, Razi Vaccine & Serum Research Institute, Karaj, Iran
Abstract
Aquatics have been considered as resource for extract of enzymes such as lipase in past years. Viscera
of rainbow trout are a very good resource for the isolation of enzyme. In this study, lipase enzyme was
purified from the foregut of rainbow trout. For purification, was used the acetone to defat, ammonium
sulphate for precipitation, ultrafiltration to concentration and chromatography of gel filtration in order
to isolation. Enzyme activity with Nitrophenyl palmitate as substrate and protein of this enzyme by
Lowry method were assessed in the different steps of purification. In this study, parameters such as
purification efficiency and the purified was examined. The results showed that in the last step of
purification, enzyme activity and enzyme protein were 6.171 U/ml and 0.53 mg/ml, respectively,
purification efficiency was 7.53% and the purified was 5.49. The molecular weight of lipase was
determined by SDS-PAGE 32 KDa. Based on this research it can be concluded that the lipase in the
intestines of rainbow trout has been useful as an additive in the future can be considered related
industries and in Biotechnology Research.
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