Investigation of common carp (Cyprinus carpio) genetic diversity in Iranian waters of the Caspian Sea by microsatellite markers

Document Type : Research Paper

Authors

1 M.Sc., Biotechnology Department, Ecology Research Center of Caspian Sea, Sari, Iran

2 Professor, Iranian Fisheries Research Organization, Tehran, Iran

3 Expert, Biotechnology Department, Ecology Research Center of Caspian Sea, Sari, Iran

Abstract

Population genetic structure of common carp (Cyprinus carpio) were examined by 120 samples from
Gilan, Mazandaran and Golestan coastal using 10 loci microsatellite. Eight of ten microsatellite loci
were polymorphism. The mean of observed and effective allele number was 7.08 and 4.29 respectively
and also the maximum of observed and expected heterozygosity was 0.95 and 0.92 respectively. It was
seen that specimens from all regions and loci (exception locus Syp8) were not in Hardy-Weinberg
Equibrium in all of the loci (P<0.05). Based on Analysis of Molecular Variance (AMOVA), the
maximum FST (0.028) and minimum Nm (3.7) was between Golestan and Mazandaran. Also, the
minimum FST and maximum Nm was between Gilan and Mazandaran. Highest genetic distance was
between Golestan and Mazandaran (0.084) and lowest genetic distance was 0.33. As result, there is a
significant genetic divergence between some of samples (P<0.01). Therefore, there are at least three
genetic group of Cyprinus carpio in Southern part of the Caspian Sea.

Keywords

Main Subjects

References

[1].   Alarcon, J.A., Magoulas, A., Alvarez, M.C., 2004. Genetic comparison of wild and collative European population of the gill head sea bream. Aquaculture 230, 65-80.
[2].   Bartfai, R., Egedi, S., Yue, G.H., Kovacs, B., Urbanyi, B., Tamas, G., Horvath, L., Orban, L., 2003. Genetic analysis of two common carp brood stocks by RAPD and microsatellite markers. Aquaculture 219, 157-167.
[3].   Brighitte, J., Hansen, M., Loeschcker, V., 2005. Microsatellite DNA analysis of northern pike (Esox lucius) populations: Insights into the genetic structure and demographic history of a genetically depauperate specious. Biology Journal of Linnaean Society 84, 1-11.
[4].   Cataudella, S., Sola, L., Corti, M., Arcangeli, R., La Rosa, G., Mattoccia, M., Coboldi Sbordoni, M., Sbordoni, V., 1987. Cytogenetic, genic and morphometric characterization of groups of common carp. Cyprinus carpio. In: Tiews, K. (Ed), Proc. World Symp. On Selection, Hybridization, and Genetic Engineering in Aquaculture, Bordeaux 27-30 May 1986. Schriften der Bundesnstalt fur Fisheries Hamburg 1, 113-129.
[5].   Csizmadia, C., Jeney, Z., Szerencses, I., Gorda, S., 1995. Transferrin polymorphism of some race in a live gene bank of common carp. Aquaculture 129, 193-198.
[6].   Dahle, G., Jorstad, K.E., Rusaas, H.E., Ottera, H., 2006. Genetic characteristics of brood stock collected from four Norwegian coastal cod (Gadus morpha) populations. Journal of Fish Biology  63, 209-215.
[7].   Daryanabard, G., Abdolmaleki, S., Bandani, A., Kor, D., 2007. Stock assessment of bony fish in the Caspian Sea. Fisheries research organization of Iran, 89 pp.
[8].   Desvignes, J.F., Laroche, J., Durand, J.D.,  Bouvet, Y., 2001. Genetic variability in reared stocks of common carp (Cyprinus carpio L.) based on allozymes and microsatellites. Aquaculture 194, 291-301.
[9].   Dimsoski, P., Toth, G.P., Bagley, M.J., 2000. Microsatellite characterization in central stoneroller Camp stoma anomalous (Pisces: Cyprinidae). Molecular Ecology 9, 2187–2189.
[10].              Froufe, E., Magyary, L., Lehocky, I., Weiss, S., 2002. mtDNA sequence data supports an Asian ancestry and single introduction of the common carp into the Danube Basin. Journal of Fish Biology  61, 301-304.
[11].              Fevolden,  S.E., Pogson, G.H., 1997. Genetic divergence at the synaptophysin locus among Norwegian  coastal and north–east Arctic population of   Atlantic Cod. Journal of  Fish Biology 51, 895-908.
[12].              Ghaninejad, D., Sayadborani, M., Pourgholami, A., Fazli, H., Abasi, K., Bandani, A., 2002. Stock assessment of bony fish in the Caspian Sea. Fisheries research organization of Iran, 95 pp.     
[13].              Ghelichpour, M., Shabani, A., Shabanpourm B., 2008. Genetic diversity of two populations of Common carp in Gharasu and regions using microsatellite markers. Taxonomy and Biosystematics 5, 41-48.
[14].              Helfman, GS., Collet, B.B., Facey, D.E., 1997. The diversity of  fishes. Blackwell Science , Malden, MS, 258 pp.
[15].              Jaime, C., Ania, P., Miguel, H., , Carmen, B., 2005. A microsatellite marker tool for parentage analysis in Senegal sole (Solea senegalensis): Genotyping errors, null alleles and conformance to theoretical assumptions. Aquaculture 261,1194 –1203.
[16].              Kitanishi, S., Yamamoto, T., Higashi, S., 2008. Microsatellite variation reveals fine-scale genetic structure of masu salmon, Oncorhynchus masou, within  the Atsuta River. Ecology of Freshwater Fish 32,1-7.
[17].              Kirpichnikov, V.S., 1999. Methods and Effectiveness of Rop-sha Carp Breeding. ommunication I. Breding Aims, Original Forms and Cross System, Russian journal of genetic 8, 65–72.
[18].              Kohlmann, K., Gross, R., Murakaeva, A., Kersten, P., 2003. Genetic variation and structure of common carp populations throughout the distribution range inferred from allozyme, microsatellite and mtDNA marker. Aquatic Living Resource  16,421-431.
[19].              Laloei, F., Rezvani gilkolaei, S., Fatemi, S.M.R., Taghavi, M.J., 2008. Investigation of population genetic structure of Common carp in the south Caspian Sea using mtDNA method (PCR-RFLP). Iranian Scientific Fisheries Journal 17, 89-102. 
[20].              Macaranas, J. M., Sato, J., Fujio, Y., 1986. Genetic characterization of culture populations of Japanese common carp. Tohoku Journal of Agricultural Research 37, 21-29.
[21].              Nei, M., 1972. Genetic distance between populations. American Naturalist 106, 283-292.
[22].              Nei, M., 1978. Estimation of average heterozygosity and genetic distance from a small number of individuals Genetics. American Naturalist 89,583-590.
[23].              Norris, A.T., Bradley, D.G., Cunning ham, E.D., 1999. Microsatellite genetic variation between and within farmed and wild Atlantic salmon populations. Department of Genetics, Trinity College Dublin, Ireland 247-264.
[24].              Peakall, M., Smouse, P.E., 2006. Gene Alex 6: Genetic analysis in Excel .Population genetic software for teaching and research. Molecular Ecology Resources 6, 288-295.      
[25].              Skaala, Q., Hoyheim, B., Glovera, K., Dahle, G., 2005. Microsatellite analysis in domesticated and Wild Atlantic salmon (Salmo salar L.): Allelic diversity and identification of individuals. Aquaculture  240, 131-143.
[26].              Sumantadinata, K., Taniguchi, N., 1990. Comparison of electrophoretic allele frequencies and genetic variability of common carp stocks from Indonesia and Japan. Aquaculture 88, 263-271.
Tanck, M.W.T., Baars, H.C.A., Kohlmann, K., Van der Poel, J.J., Komen, J., 2001. Genetic characterization of wild Dutch common carp (Cyprinus carpio L.). Agriculture Resource 31, 779-783.
[27].              Thai,  B,T., Pham, T.A., Austin, G.M., 2006. Genetic diversity of common carp in Vietnam using direct sequencing and SSCP analysis of the mitochondrial DNA control region. Aquaculture 258, 228-240.
[28].              Thai, B.T., Burridge, C.P.,  Austin, C.M., 2007. Genetic diversity of common carp (Cyprinus carpio L.) in Vietnam using four microsatellite loci. Aquaculture  269, 174–186.
[29].              Turner, F., Dowling, T.E., Broughton, R.E., gold, J.R., 2004. Variable microsatellite marker amplify across divergent lineages of Cyprinidae fishes. Conservation Genetic  5, 279-281.
[30].              Zhou, J.F., Wu, Q.J., Ye, Y.Z., Tong, J.G., 2003. Genetic divergence between Cyprinus carpio carpio and Cyprinus carpio haematopterus as assessed by mitochondrial DNA analysis, with emphasis on origin of European domestic carp. Genetic 119, 93-97.
 

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