Antibacterial activity of purified extracts of microalgae Chlorella vulgaris isolated from Chabahar Bay

Document Type : Research Paper

Authors

1 Assistant Prof. Department of Marine Biology, Facualty of Marine Sciences, Chabahar Maritime University, Chabahar, Iran

2 M s student, Department of Marine Biology, Facualty of Marine Sciences, Chabahar Maritime University, Chabahar, Iran

Abstract

Microalgae are important biological resources in a wide range of applications of biotechnology. In this study, microalgae Chlorella vulgaris isolated from Chabahar Bay and its antibacterial activity of organic extracts against three strains of Gram-negative bacteria; E. coli, Proteus vulgaris, Vibrio chlorella and two strains of Gram-positive bacteria Listeria monocytogenes and Staphylococcus aureus was examined. Ethanol and chloroform extracts obtained by disk diffusion and successive dilution methods for determining the minimum inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) were determined. Based on the results, the ethanol extracts has greatest effect on bacterium V. chlorella and the MBC for E. coli bacteria. The chloroform extract showed the greatest effect on the bacterium P. vulgaris and with different concentrations and minimal bactericidal concentration for all tested bacteria were obtained. Overall Iranian local microalgae Chlorella vulgaris has a strong antibacterial effect and Chloroform extract against ethanol extract has a more effect on the bacteria.
 
 
 
 

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References

 
Beena, B. N., & Krishnika, A. (2011). Antibacterial activity of freshwater Microalga (Sc e n e d e s m u s sp.) against three bacterial strains. Journal of Biosciences research, 2(4), 160-165
Borowitzka, M. A. (1999). Patents. Journal of Applied Phycology, 11(6), 599-603
Chu, Wan-Loy. (2012). Biotechnological applications of microalgae. International e-Journal of Science, Medicine & Education, 6, S24-S37.
Pesando D & Gnassia-Garelli M. (1979). Partial characterization of a specific antibiotic, antifungal substance isolated from the marine diatom Chaetoceros lauderi Ralfs CC. Marine Algae in Pharmaceutical Science, 447-459.
Debro, L. H., & Ward, H. B. (1979). Antibacterial Activity of Freshwater Green Algae. Planta Med, 36(08), 375-378
Jayshree A., Jayashree Sh., & Thangaraju N. (2012). Phytochemical Screening and Antimicrobial Activity of Chlorella Vulgaris Beijerinck International Journal of Current Research and Review, 4(7), 33-38
Kartal, M., Yıldız, S., Kaya, S., Kurucu, S., & Topçu, G. (2003). Antimicrobial activity of propolis samples from two different regions of Anatolia. Journal of Ethnopharmacology, 86(1), 69-73
Kellam, Stephen J, & Walker, John M. (1989). Antibacterial activity from marine microalgae in laboratory culture. British Phycological Journal, 24(2), 191-194
Mala, R., Sarojini, M., Saravanababu, S., & Umadevi, G. (2009). Screening for  Antimicrobial Activity of Crude extracts of Spirulina Platensis. Journal of Cell and Tissue Research, 9(3), 1951-1955
Nostro, A, Germano, MP, D’angelo, V, Marino, A, & Cannatelli, MA. (2000). Extraction methods and bioautography for evaluation of medicinal plant antimicrobial activity. Letters in Applied Microbiology, 30(5), 379-384
Ördög, V, Stirk, W. A., Lenobel, R., Bancířová, M., Strnad, M., Van Staden, J, . . . Németh, L. (2004). Screening microalgae for some potentially useful agricultural and pharmaceutical secondary metabolites. Journal of applied phycology, 16(4), 309-314
Perry, J. J., Staley, James T., & Lory, Stephen. (2002). Microbial Life, Sinauer Associates, Inc.
Pratt, R, Daniels TC, Eiler JB, Gunnison JB, & WD, Kumler. (1944). Chlorellin, nN Antibacterial Substance from Chlorella. Science, 99, 351-352
Priya, S. (2012). Analysis of value–added biochemical compounds and antimicrobial activity of green algae Chlorella vulgaris. Journal of Chemical and Pharmaceutical Research 4(5), 2577-2579
Uma, R., Sivasubramanian, V.  & Niranjali D. S. ( 2011). Preliminary phycochemical analysis and in vitro antibacterial screening of green micro algae, Desmococcus Olivaceous, Chlorococcum humicola and Chlorella vulgaris. Journal of Algal Biomass Utilization, 2(3), 74-81
Ragupathi, R. K., Rengasamy A., & Radjassegarin A. (2012). Chemical composition and antibacterial activity of Indian seagrasses against urinary tract pathogens. Food Chemistry, 135(4), 2470-2473
Rosaline, X. D., kumar, S., Rajendran, Sh. K., & Janarthanan, S. (2012). Screening of selected marine algae from the coastal Tamil Nadu, South India for antibacterial activity. Asian Pacific Journal of Tropical Biomedicine, 2(1, Supplement), S140-S146
Victoria, B., Doina P. B., Gabriela R., Corneliu A., & Ciprian F. B. (2009). The antibacterial activity evaluation of Cystoseira barbata biomass and some alginates upon bacteria from oropharyngeal cavity. Romanian Biotechnological Letters, 14(6), 4851-4857
 
Journal of Fisheries
Volume 68, Issue 3 - Serial Number 3
October 2015
Pages 437-445

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