Performance of Zeolite-PEI antibacterial filter in breeding tanks of Giant freshwater prawn Macrobrachium rosenbergii

Document Type : Research Paper

Authors

1 Ph. D Graduate,, Department of Fisheries, Faculty of Natural Resources, University of Tehran, Karaj, Iran

2 Professor, Department of Fisheries, Faculty of Natural Resources, University of Tehran, Karaj, Iran

3 Associate Professor, Department of Fisheries, Faculty of Natural Resources, University of Tehran, Karaj, Iran

10.22059/jfisheries.2022.334749.1297

Abstract

Use of effective substances to inhibit bacteria in the aquatic environment due to indiscriminate and long-term use of antibacterial substances such as antibiotics seems to be necessary. Polyethyleneimine (PEI) is a synthetic polymer with antibacterial properties and chemical formula (C2H5N)n which consists of many side groups of NH2, the use of this polymer due to its antibacterial property in binding with different substrates can be considered. Among these substrates that are widely used in aquaculture is Zeolite. In this study, zeolite was coated with PEI polymer with two concentrations of 1 and 2 mg/l and after confirmation by Fourier transform infrared spectroscopy (FTIR) analysis is placed inside the filters. The function of the filters was determined by measuring the bacterial load of the water, survival and growth of post larvae. The results showed that negative control, treatment of zeolite modified with concentration I, treatment of zeolite modified with concentration II of PEI polymer, treatment of natural zeolite and positive control at 104 CFU/ml Aeromonas hydrophila bacteria on the 10 days of the experiment were grew 50, 125, 125, 348 and 380 colony/100 ml in TSI medium, which shows a decrease of 67.1% of the bacterial load in both modified treatments with concentrations I and II of PEI polymer compared to the positive control. Also, the survival rate of postlarvae showed a significant increase in the treatment of modified zeolite with concentration I and II compared to the negative control (P<0.05). However, there was no significant difference between treatments in dry weight and length (P>0.05). Finally, after summarizing and comparing the results of this study with others, it can be concluded that the use of zeolite modified with PEI polymer in the filtration system of giant freshwater prawn Macrobrachium rosenbergii post larval rearing tanks has a potential effect on bacterial reduction of Aeromonas hydrophila bacteria.

Keywords

References

Adanir, D., Turutoglu, H., 2007. Isolation and antibiotic susceptibility of Aeromonas hydrophila in a carp (Cyprinus carpio) hatchery farm. Bulletin of the Veterinary Institute in Pulawy 51(1), 361-364.
Agnihotri, S., Mukherji, S., Mukherji, S., 2013. Immobilized silver nanoparticles enhance contact killing and show highest efficacy: elucidation of the mechanism of bactericidal action of silver. Nanoscale 5(4), 7328-7340.
Alishiri, M., 2017. Application of modified zeolite with polyethylenimine Polymer and use against Aeromonas hydrophila in water filtration system of tank in freshwater post larvae prawn (Macrobrachium rosenbergii) under transportation stress. M.Sc. thesis. Fish culture and fisheries group. University of Tehran. Iran. 101 p. (In Persian)
Azevedo, P., Ramalho, A., Silva, R., Teixeira-Santos, C., Pina-Vaz1, A.G., 2014. Polyethyleneimine and polyethyleneimine-based nanoparticles: novel bacterial and yeast biofilm inhibitors. Journal of Medical Microbiology 63(2), 1167-1173.
Boyd, E., Tucker, S., 1998. Pond Aquaculture Water Quality Management. Kluwer Academic Publishers, Boston. 711 p.
Bright, K., Gebra, C., Rusin, P., 2002. Rapid reduction of Staphylococcus aureus populations on stainless steel surfaces by zeolite ceramic coatings containing silver and Zinc ions. Journal of Hospital Infection 52(3), 302-309.
Gholami, M., Nazari, Sh., Farzadzika, M., Majideh, Gh., Alizadeh, S., 2016. Activation of antibacterial effect of Polyamidoamine nano dendrimer in aquatic environment. Journal Faculty of Medicine 74(3), 159-167. (In Persian)
Green, T., Mark A., 2011. Immobilized Antimicrobial Agents: A Critical Perspective. Science against microbial pathogens: communicating current research and technological advances. pp. 84-98.
Inoue, Y., Hoshino, M., Takahashi, H., Noguchi, T., Murata, T., Kanzaki, Y., Hamashima, H., Sasatsu, M., 2002. Bactericidal activity of Ag-zeolite mediated by reactive oxygen species under aerated conditions. Journal of Inorganic Biochemistry 92(3), 37-42.
Izanloo, H., Ahmadi Jebelli, M., Nazari, S., Safavi, N., Tashayoe, HR., Majidi, G., 2014. Studying the antibacterial effect of polyamidoamine dendrimeron some of the gram-negative and gram-positive bacteria. Journal of Arak University of Medical Sciences 17(9), 1-10.
Izanloo, H., Nazari, S., Ahmadi Jebelli, M., Alizadeh, M., 2015. Studying the Polypropylenimine-G2 (PPI-G2) Dendrimer Performance in Removal of Escherichia coli, Proteus Mirabilis, Bacillus Subtilis and Staphylococcus Aureus from Aqueous Solution. Journal of Arak University of Medical Sciences 18(6), 8-16.
John, G., Thomas, R., Roberts, P., 2015. Highly Crosslinked Polyethylene in Total Hip Arthroplasty Decreases Long-term Wear: A Double-blind Randomized Trial. Clinical Orthopaedics and Related Research 473(4), 432-438.
Khoobi, M., Motevalizadeh, F., Asadgol, Z., Forootanfar, H., Shafiee, A., Faramarzi, M., 2014. Polyethyleneimine-modified superparamagnetic Fe3O4 nanoparticles for lipase immobilization: Characterization and application. Materials Chemistry and Physics 149(2), 77-86.
Lin, J., Qiu, S.Y., Lewis, K., Klibanov, A.M., 2002. Bactericidal Properties of Flat Surfaces and Nanoparticles Derivatized with Alkylated Polyethylenimines. Biotechnology Progress 18(2), 1082-1086.
Matyar, F., Dincers, S., Kaya, A., Colak, O., 2004. Prevalence and resistance to antibiotics in Gram negative bacteria isolated from retail fish in Turkey. Annals of Microbiology 54(3), 151-160.
Milovic, N.M., Wang, J., Lewis, K., Klibanov, A.M., 2005. Immobilized N-Alkylated Polyethylenimine Avidly Kills Bacteria by Rupturing Cell Membranes with No Resistance Developed. Biotechnology and Bioengineering 90(4), 715-722.
Rai, M., Yada, A., Gade, A., 2009. Silver nanoparticles as a new generation of antimicrobials. Biotechnology Advances 27(1), 76-83.
Rivera-Garza, M., Olguin, M.T., Garcia-Sosa, I., Alcantara, D., Rodriguez-Fuentes, G., 2000. Silver supported on natural Mexican zeolite as an antibacterial material. Microporous and Mesoporous Materials 39(6), 431-444.
Sarkheil, M., Sourinejad, I., Mirbakhsh, M., Kordestani, D., Johari, A., 2016. Application of silver nanoparticles immobilized on TEPA-Den-SiO2aswater filter media for bacterial disinfection in culture of Penaeidshrimp larvae. Aquacultural Engineering 74(1), 17-29.
Silapajarn, O., Silapajarn, K. and Boyd, C., 2006. Evaluation of zeolite products used for aquaculture in Thailand. Journal of the World Aquaculture Society 37 (1), 136_138.
Strydom, S., Rose, W., Otto, D., Liebenberg, W., Villiers, M., 2013. Poly (amidoamine) dendrimer-mediated synthesis and stabilization of silver sulfonamide nanoparticles with increased antibacterial activity. Nanomedicine 9(1), 85-93.
Timofeeva, L., Kleshcheva, N., 2011. Antimicrobial Polymers: Mechanism of Action, Factors of Activity, and Applications. Microbial Biotechnology 89(4), 475-492.
Zendehdel, M., Solimannejad, M., 2013. Interaction between NaY Zeolite and boric Acid, a preliminary computational study. Chemistry of Solid Materials 1(2), 57-63.
Journal of Fisheries
Volume 76, Issue 1
March 2023
Pages 153-164

Files

Share

How to cite

Statistics