Effect of carbon and nitrogen ratio (C:N) manipulation on growth performance, immune response and stress index of common carp (Cyprinus carpio) acute exposure to ammonia stress in biofloc system

Document Type : Research Paper

Authors

1 M.Sc. Student of Fisheries, Faculty of Agriculture and Natural Resources, Gonbad Kavos University, Golestan, Iran

2 Assistant Professor of Fisheries Department, Faculty of Agriculture and Natural Resources, Gonbad Kavos University, Golestan, Iran

3 Associate Professor of Fisheries Department, Faculty of Agriculture and Natural Resources, Gonbad Kavos University, Golestan, Iran

4 Assistant Professor of Biology Department, Faculty of Basic and Technical and Engineering Sciences, Gonbad Kavos University, Golestan, Iran

10.22059/jfisheries.2023.353714.1365

Abstract

Biofloc is a novel technology that can improve aquaculture production and also pursue sustainable aquaculture goals. In this experiment, the impact of the different C/N ratios on water quality, growth performance and immune response of Cyprinus carpio (initial weight; 11.75±0.53g) reared under biofloc conditions were investigated. Four levels of C/N ratios, using the addition of a carbon source (sugar), 10, 15 and 20 and the control without adding carbon (Control group with 40-50% daily water exchange) were consisted of the experimental treatments. After 8 weeks, fish were subjected to acute ammonia stress (0.5mg/L) for 6 hrs. Stress indicators such as glucose and cortisol were measured and statistically compared before and after exposure to ammonia. Total ammonia nitrogen (TAN) concentration was significantly higher in C10. The growth of fish was significantly higher in C15 while the feed conversion ratio had no statistically significant difference. Serum immunoglobulin, lysozyme, ACH50 activities were significantly higher in the C/N groups than in the control. Stress indicators (glucose and cortisol) showed no significant change before exposure, while after ammonia stress, their levels increased in all groups and the highest level was observed in the control group. Overall, biofloc (C/N manipulation) had an anti-stress effect, and the culture of common carp in the biofloc system with the lowest daily water exchange could be recommended.

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References

Adineh, H., Naderi, M., Hamidi, M.K., Harsij, M., 2019. Biofloc technology improves growth, innate immune responses, oxidative status, and resistance to acute stress in common carp (Cyprinus carpio) under high stocking density. Fish and Shellfish Immunology 95, 440-448.
Adineh, H., Naderi, M., Jafaryan, H., Khademi Hamidi, M., Yousefi, M., Ahmadifar, E., 2023. Interactive effects of stocking density and dietary protein level on some growth parameters, hematology and immune response of juvenile common carp (Cyprinus carpio) to Aeromonas hydrophila in modern biofloc System. Journal of Aquatic Physiology and Biotechnology 10(4), 95-121 (In Persian).
Adineh, H., Naderi, M., Jafaryan, H., Khademi Hamidi, M., Yousefi, M., Ahmadifar, E., 2022. Effect of Stocking Density and Dietary Protein Level in Biofloc System on the Growth, Digestive and Antioxidant Enzyme Activities, Health, and Resistance to Acute Crowding Stress in Juvenile Common Carp (Cyprinus carpio). Aquaculture Nutrition, doi.org/10.1155/2022/9344478.
Adineh, H., Naderi, M., Yousefi, M., Khademi Hamidi, M., Ahmadifar, E., Hoseini, S.M., 2021. Dietary licorice (Glycyrrhiza glabra) improves growth, lipid metabolism, antioxidant and immune responses, and resistance to crowding stress in common carp, Cyprinus carpio. Aquaculture Nutrition 27(2), 417-426.
Ahmadifar, E., Kalhor, N., Yousefi, M., Adineh, H., Moghadam, M.S., Sheikhzadeh, N., Moonmanee, T., Hoseinifar, S.H., Van Doan, H., 2022. Effects of dietary Plantago ovata seed extract administration on growth performance and immune function of common carp (Cyprinus carpio) fingerling exposed to ammonia toxicity. Veterinary Research Communications47, 731–744.
APHA, 1998. Standard Methods for the Examination of the Water and Wastewater, 22nd ed. American Public Health Association, Washington, DC.
Asaduzzaman, M., Wahab, M.A., Verdegem, M.C.J., Huque, S., Salam, M.A., Azim, M.E., 2008. C/N ratio control and substrate addition for periphyton development jointly enhance freshwater prawn Macrobrachium rosenbergii production in ponds. Aquaculture 280(1-4), 117-123.
Avnimelech, Y., 1999. Carbon/nitrogen ratio as a control element in aquaculture systems. Aquaculture 176 (3-4), 227-235.
Avnimelech, Y., 2007. Feeding with microbial flocs by tilapia in minimal discharge bioflocs technology ponds. Aquaculture 264(1), 140-147.
Avnimelech, Y., 2009. Biofloc technology: a practical guide book. World Aquaculture Society.
Azim, M.E., Little, D.C., 2008. The biofloc technology (BFT) in indoor tanks: water quality, biofloc composition, and growth and welfare of Nile tilapia (Oreochromis niloticus). Aquaculture 283(1-4), 29-35.
Azimi, A., Jafaryan, H., Harsij, M., Gholipour, H., Patimar, R., 2017. Effect of C/N different ratios on water quality parameters and growth performance of common carp (Cyprinus carpio) fingerlings in biofloc system. Journal of Aquaculture Development 10(4), 75-89. (In Persian)
Azimi, A., Shekarabi, S.P.H., Paknejad, H., Harsij, M., Khorshidi, Z., Zolfaghari, M., Zakariaee, H., 2022. Various carbon/nitrogen ratios in a biofloc-based rearing system of common carp (Cyprinus carpio) fingerlings: Effect on growth performance, immune response, and serum biochemistry. Aquaculture 548, 737622.
Bakar, N.S.A., Nasir, N.M., Lananan, F., Hamid, S.H.A., Lam, S.S., Jusoh, A., 2015. Optimization of C/N ratios for nutrient removal in aquaculture system culturing African catfish, (Clarias gariepinus) utilizing Bioflocs Technology. International Biodeterioration and Biodegradation 102, 100-106.
Barton, B.A., 2002. Stress in fishes: a diversity of responses with particular reference to changes in circulating corticosteriods. Integrative and omparative, Biology 42(3), 517-525.
Borges, A., Scotti, L.V., Siqueira, D.R., Jurinitz, D.F., Wassermann, G.F., 2004. Hematologic and serum biochemical values for jundia (Rhamdia quelen). Fish Physiology and Biochemistry 30, 21-25.
Boyd, C.E., Tucker, C.S., 2012. Pond aquaculture water quality management. Springer-Verlag New York. 700 p.
Browdy, C.L., Ray, A.J. Leffler, W., Avnimelech, Y., 2012. Biofloc-based aquaculture systems. In: Aquaculture Production Systems. Eds. Tidwell, J., John Wiley and Sons, Inc, pp: 278-307.
De Schryver, P., Crab, R., Defoirdt, T., Boon, N., Verstraete, W., 2008. The basics of bio-flocs technology: the added value for aquaculture. Aquaculture 277(3-4), 125-137.
Debbarma, R., Biswas, P., Singh, S.K., 2021. An integrated biomarker approach to assess the welfare status of Ompok bimaculatus (Pabda) in biofloc system with altered C/N ratio and subjected to acute ammonia stress. Aquaculture 545, 737184.
Dosdat, A., Ruyet, J.P., Coves, D., Dutto, G., Gasset, E., Roux, A., Lemarie, G., 2003. Effect of chronic exposure to ammonia on growth: food utilization and metabolism of the European sea bass (Dicentrarchus labrax). Aquatic Living Resources 16(6), 509-520.
Ellis, A.E., 1990. Lysozyme assays. In: Stolen, J.S., Fletcher, T.C., Anderson, D.P., Robertson, B.S., Van Muiswinkel, publication. pp: 101-103.
Emerenciano, M., Ballester, E.L.C., Cavalli, R.O., Wasielesky, W., 2012. Biofloc technology application as a food source in a limited water exchange nursery system for pink shrimp Farfantepenaeus brasiliensis (Latreille, 1817). Aquaculture Research 43(3), 447-457.
Haghparast, M. M., Alishahi, M., Ghorbanpour, M., Shahriari, A., 2020. Evaluation of hemato-immunological parameters and stress indicators of common carp (Cyprinus carpio) in different C/N ratio of biofloc system. Aquaculture International 28(6), 2191-2206.
Hargreaves, J.A., 2013. Biofloc Production Systems for Aquaculture, pp: 1-11.
Hegazi, M.M., Attia, Z.I., Ashour, O.A., 2010. Oxidative stress and antioxidant enzymes in liver and white muscle of Nile tilapia juveniles in chronic ammonia exposure. Aquatic Toxicology 99(2), 118-125.
Kim, J.H., Park, H.J., Hwang, I.K., Han, J.M., Kim, D.H., Oh, C.W., Kang, J.C., 2017. Alterations of growth performance, hematological parameters, and plasma constituents in the sablefish, Anoplopoma fimbria depending on ammonia concentrations. Fisheries and Aquatic Sciences 20, 1-6.
Mangang, Y.A., Pandey, P.K., 2021. Hemato-biochemical responses and histopathological alterations in the gill and kidney tissues of Osteobrama belangeri (Valenciennes, 1844) exposed to different sub-lethal unionized ammonia. Aquaculture 542, 736887.
Minabi, K., Sourinejad, I., Alizadeh, M., Ghatrami, E.R., Khanjani, M.H., 2020. Effects of different carbon to nitrogen ratios in the biofloc system on water quality, growth, and body composition of common carp (Cyprinus carpio L.) fingerlings. Aquaculture International 28, 1883-1898.
Mirghaed, A.T., Fayaz, S., Hoseini, S.M., 2019. Effects of dietary 1, 8-cineole supplementation on serum stress and antioxidant markers of common carp (Cyprinus carpio) acutely exposed to ambient ammonia. Aquaculture 509, 8-15.
Mugnier, C., Zipper, E., Goarant, C., Lemonnier, H., 2008. Combined effect of exposure to ammonia and hypoxia on the blue shrimp Litopenaeus stylirostris survival and physiological response in relation to molt stage. Aquaculture 274 (2-4), 398-407.
Najdegerami, E. H., Tukmechi, A., 2022. Poly‐β‐hydroxybutyrate concentration, microbial enzymes activity, and nutritional value in biofloc system using different carbon sources and C/N ratios in common carp, Cyprinus carpio culture. Journal of the World Aquaculture Society, doi.org/10.1111/jwas.12922
Najdegerami, E.H., Bakhshi, F., Lakani, F.B., 2016. Effects of biofloc on growth performance, digestive enzyme activities and liver histology of common carp (Cyprinus carpio L.) fingerlings in zero-water exchange system. Fish physiology and Biochemistry 42, 457-465.
Ray, A.J., Lotz, J.M., 2014. Comparing a chemoautotrophic-based biofloc system and three heterotrophic-based systems receiving different carbohydrate sources. Aquacultural Engineering 63, 54-61.
Robert, M., Crosby, D.M., Brunson, M.W., 1992. Ammonia in Fish Ponds. Southern Regional Aquaculture Center Publication, No. 463.
Sinha, A.K., AbdElgawad, H., Giblen, T., Zinta, G., De Rop, M., Asard, H., Blust, R., De Boeck, G., 2014. Anti-oxidative defences are modulated differentially in three freshwater teleosts in response to ammonia-induced oxidative stress. PLoS One 9(4), 95319.
Sinha, A.K., Kapotwe, M., Dabi, S.B., da Silva Montes, C., Shrivastava, J., Blust, R., De Boeck, G., 2016. Differential modulation of ammonia excretion, Rhesus glycoproteins and ion-regulation in common carp (Cyprinus carpio) following individual and combined exposure to water borne copper and ammonia. Aquatic Toxicology 170, 129-141.
Sunyer, J.O., Tort, L., 1995. Natural hemolytic and bactericidal activities of sea bream Sparus aurata serum are affected by the alternative complement pathway. Veterinary Immunology and Immunopathology 45(3-4), 333-345.
Taheri Mirghaed, A., Fayaz, S., Hoseini, S.M., 2019. Effects of dietary 1, 8-cineole supplementation on serum stress and antioxidant markers of common carp (Cyprinus carpio) acutely exposed to ambient ammonia. Aquaculture 509, 8-15.
Yousefi, M., Vatnikov, Y.A., Kulikov, E.V., Plushikov, V.G., Drukovsky, S.G., Hoseinifar, S.H., 2020. The protective effects of dietary garlic on common carp (Cyprinus carpio) exposed to ambient ammonia toxicity. Aquaculture 526, 735400.
Yu, Z., Quan, Y.N., Huang, Z.Q., Wang, H. H., Wu, L.F., 2020a. Monitoring oxidative stress, immune response, Nrf2/NF-κB signaling molecules of Rhynchocypris lagowski living in BFT system and exposed to waterborne ammonia. Ecotoxicology and Environmental Safety 205, 111161.
Yu, Z., Wu, X.Q., Zheng, L.J., Dai, Z.Y., Wu, L.F., 2020b. Effect of acute exposure to ammonia and BFT alterations on Rhynchocypris lagowski: Digestive enzyme, inflammation response, oxidative stress and immunological parameters. Environmental toxicology and pharmacology 78, 103380.
Zhao, Z., Xu, Q., Luo, L., Wang, C., Li, J., Wang, L., 2014. Effect of feed C/N ratio promoted bioflocs on water quality and production performance of bottom and filter feeder carp in minimum-water exchanged pond polyculture system. Aquaculture 434, 442-448.

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