Investigating the effect of different concentrations of lead chloride on the expression of genes effective in the immune factors of rainbow trout (Oncorhynchus mykiss)

Document Type : Research Paper

Authors

1 MSc. student, Department of Fisheries, Faculty of Natural Resources, University of Tehran, Karaj, Iran

2 Assistant professor,, Department of Fisheries, Faculty of Natural Resources, University of Tehran, Karaj, Iran

3 Professor,, Department of Fisheries, Faculty of Natural Resources, University of Tehran, Karaj, Iran

10.22059/jfisheries.2024.362092.1395

Abstract

Lead is one of the heavy metals with a toxic effect found in inland waters and breeding tanks and can cause infections and diseases in aquatic animals and humans. The purpose of this study is to investigate the effect of lead metal in juvenile rainbow trout (Oncorhynchus mykiss) and its effect on the expression of immune-related genes (MHC I, IL-6, TNFα) and heat shock protein gene (HSP70). After reproduction and rearing of fishes in lead-free water, the fry (0.5 gr) was allocated to three different treatments of lead chloride (PbCl2) with concentrations (0.25, 0.5, and 1) micromole/liter and a control group in three replicates (35 fry per replicate) for 96 hours. Then random samples were frozen in liquid nitrogen and used for gene expression testing by Real-time PCR. The results revealed that the HSP70 heat shock protein gene had significant changes (P<0.05) in two concentrations of 0.25 and 1 micromole/liter and the reverse U-shaped curve showed that this is probably due to the effect of lead hormesis. The expression of interleukin 6 and MHC I genes had a significant increase (P<0.05) in concentration, and the TNF-α gene also increased, unlike HSP70, which indicated a U-shaped curve and probably the effect of normal hormesis. The results of this study showed that lead, even in concentrations close to drinking water, can change the expression of genes caused by stress and factors related to immunity, and its effects do not end only with poisoning.

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Main Subjects

References

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Journal of Fisheries
Volume 76, Issue 4
December 2023
Pages 655-666

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