Effects of combination of turmeric and thyme hydro-alcoholic extracts on growth, nutritional indices, digestive and antioxidant enzymes activity of rainbow trout (Oncorhynchus mykiss)

Document Type : Research Paper

Authors

1 Ph.D graduate, Department of Fisheries, Faculty of Natural Resources, Urmia University, Urmia, Iran

2 Associate Professor, Department of Fisheries, Faculty of Natural Resources, Urmia University, Urmia, Iran

3 Associate Professor, Department of Microbiology, Faculty of Veterinary, Urmia University, Urmia, Iran

4 Associate Professor, Department of Agriculture, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Tehran, Iran

10.22059/jfisheries.2024.364142.1402

Abstract

The effect of dietary administration of combination of hydro-alcoholic extracts of turmeric (Curcuma longa L.) and thyme (Zataria multiflora Boiss) on growth performance, nutritional parameters, digestive enzymes activity and antioxidant properties of rainbow trout (Oncorhynchus mykiss) was investigated. Three experimental diets including basal diet (control group) and diets containing 0.5% and 1.5% of combination of turmeric and thyme (ratio of 1:2) extracts were prepared. A total of 234 juveniles of rainbow trout were randomly divided into three experimental groups in triplicates (providing 26 fish per replicate) and reared for eight weeks. Feeding diets contained 0.5 % of the extract increased the feed efficiency ratio and condition factor of rainbow trout compared to the control group (P<0.05). Supplementing 1.5 % plant extract increased the Average Daily Growth (ADG) of the fish in comparison to control group (P<0.05). Both dietary levels of the extracts increased protein productive value and lipid productive value of fish compared to control group (P<0.05). Viscerosomatic and hepatosomatic index were affected by both dietary extract levels compared to control group (P<0.05). Dietary plant extract inclusion significantly increased the activity of amylase and lipase (P<0.05). Similarly, the activity of superoxide dismutase and glutathione peroxidase significantly increased in the liver tissue of rainbow trout following feeding on diets containing the extracts, while malondialdehyde content of the tissue decreased in comparison to the control group (P<0.05). Therefore, considering the promising effects of dietary supplementation of turmeric and thyme extracts, inclusion of 0.5% of the extracts in rainbow trout feed is recommended.

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