The purpose of this study is determining of some immunity and blood serum parameters in brood stock and grower fish before and after of stripping to evaluate the effect of breeding physiological changes on immunity and serum indexes. In this study 15 male trout fish (Oncorhynchus mykiss) including 5 in the stage of grower, 5 before stripping and 5 after stripping randomly were considered. Blood samples of brood stock and grower fish after anesthesia by Dianthus caryophyllus extract were collected from a commercial farm in Haraz and sent to the laboratory. Laboratory results showed that there is a significant difference in glucose concentration of grower fish before and after stripping (P<0.05). However, average total protein in all kind of fish approximately was unchanged(P>0.05).Also, comparison of average osmolality in all fish showed that  negligible difference  is 5%.Kortizol hormone concentration after stripping compare with other fish showed a significant difference(P<0.05). The number of white blood cell in brood stock before and after stripping decreased drastically compare with grower Fish. Blood hemoglobin before stripping increased considerably in 2 spices of fish. Although the percentage of blood hematocrit decreased significantly before stripping, the reduction of this factor after stripping was approximately unchanged. Besides, MCV index in grower fish before and after stripping was more than brood stock. Finally, it can be concluded that at the moment of reproducing, the secretion of endocrine hormones in male rainbow trout completely affect fish physiology. In this study, we exanimated the measurement of mentioned indicators for managing more resistant or less sensitive and metabolism can be better.