تاثیرمکمل ال-کارنیتین روی الگوی رشد،ترکیب شیمیایی بدن، پروفایل اسید‌های چرب بدن و فعالیت فاکتور شبه انسولین (IGF-1) ماهی شانک زرد باله (Acanthopagrus latus, Houttyn)

نوع مقاله: مقاله پژوهشی

نویسنده

1. استادیار گروه شیلات، دانشکده علوم دریایی، دانشگاه دریانوردی و علوم دریایی چابهار، چابهار، ایران

چکیده

 ال کارنیتین به­عنوان یکی از مکمل­های ضروری و مهم جهت تغذیه انسان و حیوان شناخته شده است. مطالعه حاضر، به­منظور بررسی اثر مکمل ال- کارنیتین بر  عملکرد رشد ( وزن نهایی بدن، میزان رشد روزانه، ضریب تبدیل غذایی، کارایی تبدیل غذایی، میزان غذای دریافتی، میزان کارایی پروتئین، میزان کارایی چربی، میزان تولید پروتئین و میزان تولید چربی) و ترکیبات شیمیایی لاشه ( پروتئین، چربی، خاکستر و رطوبت)، پروفایل اسید­های چرب بدن و فعالیت فاکتور رشد شبه انسولین1 (IGF-1) ماهی شانک زرد باله طراحی گردید. در این تحقیق، 240 ماهی با میانگین وزنی 46/0±23/3 گرم به­صورت تصادفی در 4 گروه 60 تایی ( با سه تکرار و در هر تکرار 20 قطعه ماهی) در مخازن پلاسنیکی 60 لیتر قرار گرفتند و با رژیم غذایی محتوی 0، 400، 800 و 1200 میلی­گرم ال-کارنیتین بر کیلوگرم غذا به­صورت روزانه مورد تغذیه قرار گرفتند. بعد از 64 روز، رژیم غذایی محتوی 800 میلی­گرم ال- کارنتین بر کیلوگرم غذا منجر به افزایش معنی­دار عملکرد رشد ( به استثنای میزان رشد روزانه)، میزان پروتئین و چربی لاشه، اسید­های چرب بدن و فعالیت شبه انسولین شد. همبستگی مثبت بین فعالیت شبه انسولین و میزان رشد روزانه به استثنای رژیم غذایی محتوی  1200 میلی­گرم ال- کارنیتین بر کیلوگرم غذا مشاهده شد. با توجه به نتایج حاضر می­توان بیان نمود که رژیم غذایی  800 میلی­گرم ال- کارنیتین بر کیلوگرم غذا می­تواند منجر به افزایش رشد، کیفیت لاشه، فعالیت فاکتور رشد شبه انسولین 1 و متابولیسم لیپید در ماهی شانک زرد باله شود. 

کلیدواژه‌ها


عنوان مقاله [English]

Effects of Dietary L-Carnitine Supplementation on Growth Pattern, Body Chemical Composition, Body Fatty Acid Profile and Insulin Liked Factor- 1 (IGF-1) of Yellowfin Seabream, Acanthopagrus latus, Houttyn, 1782

نویسنده [English]

  • Paria Akbary1*
1. Assistant Professor of Fisheries Group, Marine Sciences Faculty, Chabahar Maritime University, Chabahar, Iran
چکیده [English]

L- carnitine has been recognized as a new and important dietary supplementation, essential for human and animal nutrition. This study was conducted to evaluate the use of dietary l- carnitine supplementation and its effects on the growth (body final weight, daily growth rate (DGR), feed efficiency (FE), feed conversion ratio (FCR), daily feed intake  (DFI), protein efficiency ratio (PER), lipid efficiency ratio (LPE), protein productive value (PPV), lipid productive value (LPV), body compositions (crude protein, crude lipid, ash and moisture), body fatty acid profile and IGF-1 activity in yellowfin seabream. In this study, a number of 240 fish with a mean weight of 3.23± 0.46 g were randomly allocated into four groups (three replicates, 20 fish per tank) in 60-L plastic tanks and fed with four experimental diets having carnitine with the concentrations 0, 400, 800, and 1200 mg kg-1 diet (referred as C0, C400, C800 and C1200 respectively). After 9 weeks of the feeding trial, there was a significant increase in growth performance (except for DGR), carcass quality (crude protein and crude lipid), muscle fatty acid and IGF‑1 activity in the fish fed C800 diet compared to those fed the other diets. Also a positive correlation was detected between plasma IGF-1 activity and DGR except for C1200 diet. The diet containing 800 mg kg-1 dietary L‑carnitine can have marked effects on growth yield, carcass quality, IGF-1 activity and lipid metabolism of yellowfin seabream 

کلیدواژه‌ها [English]

  • Acanthopagrus latus
  • l-carnitine
  • lipid metabolism
  • growth performance
  • Carcass Quality
  • IGF-1 activity
Akbari, M., Zakipour, E., Sourinejad, I., Azimi Rad, M., Efatpanah, E., Khanjani, M.H., 2014. Growth Performance and Body Composition of  Pikeperch (Sander lucioperca) Fingerlings  under Dietary L-Carnitine. Marine Science 5, 57-64

AOAC, 1989. Assosiation of Official Analytical Chemists (AOAC). Official Method of Analysis of the Assosiation of Official Analytical Chemists, 15th ed. Assosiation of Official Analytical Chemists, Arlington, VA, USA.

Bromage, N.R., Robert, R.G., 2001. Broodstock  management  and  egg  and  larval  quality.  Blackwell  Science. 425 p.

Chatzifotis, S., Takeuchi, T., 1997 E¡ect of supplemental carnitine on body weight loss, proximate and lipid compositions and carnitine content of Red Sea bream (Pagrus major) during starvation. Aquaculture 158, 129-140.

Chatzifotis, S., Takeuchi, T., 1997 Effect of  supplemental carnitine on body weight loss, proximate and lipid compositions and carnitine  content of Red Sea bream (Pagrus major) during starvation. . Aquaculture  129-140-158.

Chatzifotis, S., Takeuchi, T., Seikai, T., 1996 The e¡ect of dietary carnitine supplementation on growth of red sea bream (Pagrus major) fingerlings at two levels of dietary lysine. Aquaculture 147, 235-248.

Chatzifotis, S., Takeuchi, T., Seikai, T., 1995. The effect of dietaryL-carnitine on growth performance and lipid composition in red sea bream fingerlings.


Fisheries Science 61,1004-1008

Dikel, S., Ünalan, B., Eroldoğan, O.T., Özlüer Hunt, A., 2010. Effects of dietary L-carnitine supplementation on growth, muscle fatty acid composition and economic profit of rainbow trout (Oncorhynchus  mykiss) Turkish Journal of Fisheries and Aquatic Sciences 10, 173-180.

Doberenz, J., Birkenfeld, C., Kluge, H., Eder, K., 2006. Effects of L-carnitine supplementation in pregnant sows on plasma concentrations of insulinlike growth factors, various hormones and metabolites and chorion characteristics. Journal of Animal Physiology and Animal Nutrition (Berl) 90, 487-499.

Duan, C., Hirano, T., 1990. Stimulation of 35 S-sulfate uptake by mammalian insulin-like growth factor I and II in cultured cartilages of the Japanese eel,Anguilla japonica. Journal of  Experimental  Zoology 256, 347-350.

Folch, J., Lees, M., Stanley, G.H.S., 1957. A simple method for the isolation and purification of total lipids from animal tissues. Jornal of Biology and Chemistry 226, 496-509.

Harpaz, S., 2005. L-carnitine and its attributed functions in fish culture and nutrition, a review. Aquaculture 249, 3-21.

Heo, Y.R., Kang, C.W., Cha, Y.S., 2001. L-Carnitine changes the levels of insulin-like growth factors (IGFs) and IGF binding proteins in streptozotocin-induced diabetic rat. Journal of  Nutritional Science and Vitaminology (Tokyo)47, 329-334.

Jalali Haji-abadi, S.M.A., Mahboobi Soofiani, N., Sadeghi, A.A., M., C., Riazi, G., 2010. Effects of supplemental dietary L-carnitine and  ractopamine on the performance of juvenile  rainbow trout, (Oncorhynchus mykiss). Aquaculture Research 41, 1582-1591

Kita, K., Kato, S., Amanyaman, M., Okumura, J., Yokota, H., 2002. Dietary L-carnitine increases plasma insulin-like growth factor-I concentration in chicks fed a diet with adequate dietary protein level. British Poultry Science 43, 117-121.

Mohseni, M., Ozorio R.O.A., Pourkazemi, M., Bai, S.C., 2008  Effects of dietary L-carnitine supplements on growth and body composition in  beluga sturgeon (Huso huso) juveniles. Journal of Applied Ichthyology 24, 646-649.

Mohseni, M., Ozório, R.O.A., 2014. Effects of dietary L-carnitine level on growth performance, body composition and antioxidant status in beluga (Huso huso L. 1758). Aquaculture Nutrition 20, 477-485.

Nogueira, N., Cordeiro, N., Canada, P., Cruz e Silva, P., Ozorio, R.O.A., 2010. Separate and combined effects of cyclic fasting and L-carnitine supplementation inred porgy (Pagrus pagrus, L. 1758). Aquaculture Research 41, 795 - 806.

Nogueira, N., Cordeiro, N., Canada, P., Cuz e Silva, P., Ozório, R.O.A., 2011.  Separate and combined effects of cyclic fasting and L-carnitine supplementation in red porgy (Pagruspagrus, L. 1758). Aquaculture Research 41, 795-806.

Nogueira, N., Cordeiro, N., Canada, P., Cuz e Silva, P., Ozório, R.O.A., 2011. Separate and combined effects of cyclic fasting and L-carnitine supplementation in red porgy (Pagrus pagrus, L. 1758). Aquaculture Research 41, 795-806.

Ozório, R.O.A., Van Eekeren, T.H.B., Huisman, E.A., Verreth, J.A.J., 2001. Effects of dietary carnitine and protein energy: nonprotein energy ratios on growth, ammonia excretion and respiratory quotient in African catfish, Clarias gariepinus (Burchell) juveniles. Aquaculture Research 32, 406-414.

Ozrorio, R.O.A., Verreth, J.A.J., Aragao, C.R., Vermeulen, C.J., Schrama, J.W., Verstegen, M.W.A., 2003. Evaluation of dietary carnitine supplements on plasma metabolite indicesand fatty acid composition in African catfish (Clarias gariepinus) juveniles. Journal of Aquaculture in the Tropics 18, 225-238.

Safari, O., Mehraban Sang Atash, M., Paolucci, M., 2015. Effects of dietaryL-carnitine level on growth performance, immune responses and stress resistance of juvenile narrow clawed crayfish, Astacus leptodactylus leptodactylus Eschscholtz, 1823. Aquaculture 439, 20-28.

Twibell, R.G., Brown, P.B., 2000. Effects of dietary carnitine on growth rates and body composition of  hybrid striped bass (Morone saxatilis male × M.  chrysops female). Aquaculture 187, 153-161.

Wahli, T., Verlhac, V., Griling, P., Gabaudan, J., Aebischer, C., 2003. Influence of dietary vitamin C on the wound healing process in rainbow trout (Oncorhyncus mykiss). Aquaculture 225, 371-386.