Evaluation and Comparison of Effects of N-hexane and Acetone Extracts Derived from Marine Geyser (Phaulasia nigra), Marine Sponge (Cliona spp.), Carpet Anemone (Sarcophyton spp.) and Starfish (Pentaceraster spp.) on E. coli Bacteria

Mansurlakuraj, Marzieh; Rezaei Tavabe, Kamran; Mirvaghefi, Alireza; Nazemi, Melika; Nematollahi, Mohammad Ali

doi:10.22059/jfisheries.2017.63864

Evaluation and Comparison of Effects of N-hexane and Acetone Extracts Derived from Marine Geyser (Phaulasia nigra), Marine Sponge (Cliona spp.), Carpet Anemone (Sarcophyton spp.) and Starfish (Pentaceraster spp.) on E. coli Bacteria

Document Type : Research Paper

Authors

Marzieh Mansurlakuraj ¹

Kamran Rezaei Tavabe

Alireza Mirvaghefi ²

Melika Nazemi ³

Mohammad Ali Nematollahi ⁴

¹ MSC in Fisheries, Department of Fisheries, Faculty of Natural Resources, Tehran University, Karaj, Iran.

² Professor, Department of Fisheries, Faculty of Natural Resources, Tehran University, Karaj, Iran.

³ Assistant Professor, Faculty of Persian Gulf and Oman sea Ecological Research, Iranian Fisheries Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Bandar Abbas, Iran.

⁴ Associate Professor, Department of Fisheries, Faculty of Natural Resources, Tehran University, Karaj, Iran.

10.22059/jfisheries.2017.63864

Abstract

To study the pharmacological characteristics of marine natural products has led to the discovery of bioactive substances. The source of bioactive natural products biological and chemical structural properties marine environment cannot be seen of other natural products terrestrial plants and animals. This study aimed to evaluate and compare the antibiotic properties of n-hexane and acetone extracts were taken from four species of invertebrates. Geyser (Phaulasia nigra), sponge (Cliona spp.), carpet anemone (Sarcophyton spp.) and starfish (Pentaceraster spp.) species were collected from the Island lark. Then extraction was carried out by n-hexane and acetone solvents soaking. Antibacterial effects on Escherichia coli bacteria dilution method were at first Agar diffusion method and then performed to determine minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). N-hexane extracts derived from any of the four species studied had antibacterial properties. Estonia extracts derived from sponge and starfish in concentration of 10 and 40 mg/ml was bacterial growth inhibition and a concentration of 20 mg/ml acetone extracts derived from the sponge has antibacterial properties and cytotoxicity. Maximum zone of inhibition was 16.2± 1.5 mm by the sponge acetone extract and minimum zone of inhibition was 0.2± 0.3 mm by the starfish acetone extract. The results show Estonia sponge and starfish extracts contains compounds with antibacterial effect but the cytotoxicity of Estonia sponge extracts in this study much more.

Keywords

Marine sponge

Marine geyser

Marine starfish

Carpet anemone

MIC