Nutritional Effects of Black Powder Seeds (Nigella sativa) on Antibacterial Activity and Safety Mucus Indicators Caspian Roach (Rutilus caspicus)

Sahraei, Hasan; Zohieri, Fazel; Imanpou, Mohammad Reza; Pirali Zefrei, Ahmad Reza

doi:10.22059/jfisheries.2017.223483.965

Nutritional Effects of Black Powder Seeds (Nigella sativa) on Antibacterial Activity and Safety Mucus Indicators Caspian Roach (Rutilus caspicus)

Document Type : Research Paper

Authors

Hasan Sahraei ¹

Fazel Zohieri ¹

Mohammad Reza Imanpou ²

Ahmad Reza Pirali Zefrei ³

¹ M.Sc. Graduate, Department of Fisheries, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.

² Professor, Department of Fisheries, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.

³ Ph.D Student, Department of Fisheries, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.

10.22059/jfisheries.2017.223483.965

Abstract

In this study, the effect different levels of Nigella sativa feed on mucus, antibacterial activity of some enzymes’ mucus were examined the baby fish roach (‌Rutilus caspicus). 420 carp with average weight of 4/49 ± 0/11 gr on 4 group‌ control , 0.5, 1 and 2 percent seed powder per kg of diet were fed with three replications for 60 days . At the end of levels of soluble protein mucus, antibacterial activity and phosphatase alkaline by microdilution methods were measured against Gram-positive bacteria aureus Staphylococcus, Streptococcus faecalis and Gram-negative bacteria Escherchia coli and Pseudomonas aeruginosa. The showed results that the antibacterial activity of mucus with increasing levels of black seed increased and the diameter of the growth in fish fed with %2 black seed significantly increased compared with the control group(P<0/05) and the most diameter of inhibition against was observed to the bacteria Streptococcus faecalis. Also the levels soluble protein of mucus, lysozyme enzyme and phosphatase alkaline in fish fed up Nigella sativa 0.5 Ratio to the control group significantly increased (P<0/05). Minimum the growth inhibitory concentration (MIC) on a concentration of 50 microliter on ml and concentration higher concentrations of mucus, was higher the antibacterial activity and the concentration 200 microlitre per ml the most was seen antibacterial activity. Overall results that showed diet of black seed has a positive effect on the antibacterial activity is the mucus roach fish.

Keywords

Antibacterial activity

Mucus

Roach

Black seeds

Subjects