Optimization of chitosan extracted from spiny rock lobster (Panulirus homarus) using response surface method

Document Type : Research Paper

Authors

1 Department of Microbiology, Faculty of Basic Sciences, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.

2 Department of Fisheries, Ahv, C., Islamic Azad University, Ahvaz, Iran.

10.22059/jfisheries.2025.378188.1432

Abstract

Chitosan, the deacetylation product of chitin, is a natural cationic linear polysaccharide that has been recognized as one of the most promising renewable biopolymers due to its non-toxic, biodegradable, and biocompatible properties. Since chitosan extraction methods are difficult, time-consuming, and sometimes have low yields, it is necessary to optimize and determine the parameters of the extraction process to achieve the best yield. This research used the response surface method (RSM) optimization technique to achieve the highest deacetylation efficiency for extracted chitosan and reduce costs from spiny lobster waste materials. The experimental design was completely randomized in 3 levels (+1, 0, -1) with 5 independent variables as input including; HCl concentration, NaOH concentration, deacetylation time, deacetylation temperature, and demineralization time to determine the best combination of effective variables in chitosan extraction process. The results showed that the highest degree of deacetylation of lobster chitosan happens with 5.13 M HCl concentration, 11.58 h demineralization time, 0.52 NaOH, 2.99 h deacetylation time, and 74.13 °C deacetylation temperatureand. Comparing the values obtained in this experiments with the values predicted by the software had logical relationships that showing the appropriateness of the used model.

Keywords

Main Subjects

References

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Journal of Fisheries
Volume 78, Issue 2
July 2025
Pages 123-134

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