Evaluation of the effect of a strain of Lactobacillus isolated from local yogurt on growth, nutritional parameters, and survival of common carp (Cyprinus carpio)

Document Type : Research Paper

Authors

1 Department of Fisheries, Faculty of Natural Resources, Behbahan Khatam Alanbia University of Technology, Behbahan, Iran.

2 Dr. Adeli Farzanegan High School for Exceptional Talents, Behbahan, Khuzestan, Iran.

3 Department of Fisheries, Faculty of Natural Resources, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran.

10.22059/jfisheries.2026.409438.1473

Abstract

The aim of this study was to investigate the effect of probiotic Lactobacillus isolated from local yogurt of Behbahan city on growth parameters, survival and nutritional indices of common carp (Cyprinus carpio). A total of 135 fish with an average weight of 20 ± 5 g were reared in 9 100-liter tanks using three treatments (with three replications) including zero levels (control), 103CFUg-1 (M3) and 106CFUg-1 (M6) of probiotic Lactobacillus (Lactobacillus spp.) isolated from local yogurt of Behbahan city. The fish were fed daily at a rate of 3% of body weight. After the end of the research period, the growth parameters, survival and nutritional indices of the fish were examined.The results of comparing growth and survival data (percentage of survival, total weight, specific growth rate, relative weight rate and daily weight gain) indicated that both probiotic treatments showed significant improvement compared to the control group (P<0.05). However, in terms of total length and condition factor, no significant difference was observed between the treatment fed with 103 CFUg-1 probiotic and the control group. Comparison of nutritional parameters (feed conversion efficiency, feed conversion ratio, protein efficiency ratio, fat efficiency ratio) also showed that both probiotic treatments had a significant difference compared to the control group (P<0.05). Based on the results of this study, feeding common carp with a diet containing probiotic Lactobacillus isolated from local Behbahan yogurt at a rate of 106CFUg-1 probably has the potential to improve the growth of farmed carp in aquaculture systems.

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References

Abooali-Darvish-Taheri., M, Ziaei-nejad, S., Sadat Naghavi, N., 2014. The antibacterial activity of strains isolated from Epinephelus coioides in Deylam seaport of Bushehr province. Journal of Marine Biology 6(2), 55-66
Akbarnezhad, E., Yeganeh, S., Mohammadzadeh, S., 2024. Individual and combined effects of the dietary Lactobacillus casei and sodium propionate supplementation on the growth performance and immunity of zebra fish (Danio rerio). Iranian Scientific Fisheries Journal 33(4), 131-142. DOI: 10.22092/ISFJ.2024.132546.
Anderson, D.P., 1992. Immunostimulants, adjuvants, and vaccine carriers in fish: applications to aquaculture. Annual Reviewes of Fish Disease 2, 281-307. DOI: 10.1016/0959-8030(92)90067-8
Baesi, F., Aberoumand, A., Ziaei-Nejad, S., Javaheri-Baboli, M., 2017. Effects of commercial probiotic Lactobacillus on survival and growth parameters and nutritional indexes of common carp (Cyprinus carpio). Journal of Aquaculture Development 10(4), 39-49. DOI: 20.1001.1.23223545.1395.10.4.2.8
Bahrekazemi, M., 2025. Effect of dietary probiotic Lactobacillus acidophilus and polyphenol ferulic acid on non-specific immunity and antioxidant activity in goldfish (Carassius auratus). Iranian Scientific Fisheries Journal 34(1), 65-77. DOI: 10.22092/ISFJ.2025.133854
Bairagi, A., Ghos, K.S., Sen, S.K., Ray, A.K., 2004. Evaluation of the nutritive value of Leucaena leucocephala leaf meal, inoculated with fish intestinal bacteria Bacillus subtilis and Bacillus circulans in formulated diets for rohu, Labeo rohita (Hamilton) fingerlings. Aquaculture Research 35(5), 436 -446. DOI: 10.1111/j.1365 -2109.2004.01028.x
Bagheri, T., Hedayati, S.A., Yavari, V., Alizade, M., Farzanfar, A., 2008. Growth, Survival and Gut Microbial Load of Rainbow Trout (Onchorhynchus mykiss) Fry Given Diet Supplemented with Probiotic During the Two Months of First Feeding. Turkish Journal of Fisheries and Aquatic Sciences 8, 43-48.
Bakhshzad Mahmoudi, A., Farokhrouz Lashidani, M., Zamini, A., Shenavar Masuleh, A., Tehranifard, A., 2023. Growth performance and serum immune responses of the common carp (Cyprinus carpio) using Lactococcus lactis and Weissella cibaria as potential dietary probiotics. Iranian Journal Of Fisheries Sciences 22(3), 615-630. DOI: 10.22092/ijfs.2023.129475.
Holt, J.G., Krieg, N.R., Sneath, P.H.A., Staley, J.T., Williams, S.T., 2000. Bergey’s Manual of Determinative Bacteriology 9th edition. Edited by Lippincott Williams and Wilkins. USA
Honarman-Jahromi, S., Baghbani-arani, F., Yaghoubi, F., 2019. Isolation and identification of lactic acid bacteria with probiotic potential from traditional yogurt in Varamin province. .Iranian Journal of Biological Sciences 1, 9-18. DOI: 20.1001.1.17354226.1398.14.1.2.1
Jafarian, H., 2006. The effect of Bacillus bacteria as probiotics on growth, survival and digestive enzyme activity in Persian catfish larvae (Acipenser persicus) during larval rearing through enrichment with Artemia urmiana. (Artemia urmiana). Dissertation, Gorgan University of Agricultural Sciences and Natural Resources.
Jafarian, H., Taati, M., Nazarpour, A., 2007. Supplementation of probiotic bacilli mixture in Daphnia magna flour for feeding rainbow trout larvae (Oncorhynchus mykiss) based on growth performance. Paper presented at the 1rd National Conference on Applied Microbiology of Iran, Alzahra University, Tehran, 20-21 July 2007.
Jafarian, H., Adine, H., Ahmadii, M., 2007. The potential of probiotic bacillus on the growth performance of Rainbow trout (Oncorhynchus mykiss) fry in use of Daphnia meal. International training course and workshop.Islamic Azad university of Ghaemshahr. 5 September. Ghaemshahr-Iran.
Khosravi Katuli, K., Jafarian, H., Abdollahi, D., Tavana, S., 2014. Performance evaluation of combined probiotic Bacillus and yeast Saccharomyces cerevisiae isolated from the digestive tract of Huso huso on growth factors and biochemical body extracts improvement and increased resistance of Silver Carp (Hypophthalmichthys molitrix) larvae in the face of stressors. Journal of Fisheries 66(4), 441-451. DOI: 10.22059/jfisheries.2014.36437
Koliaee, Z., Aberoumand, A., Ziaei-nejad, S., Javahery Baboli, M., 2016a. The effect of probiotic bacteria of bacillus subtilis on fatty acid profiles of common carp (Cyprinus carpio) fillet. Journal of Utilization and Cultivation of Aquatics 5(2), 17-29. DOI: 10.22069/japu.2016.3597
Koliaee, Z, Aberoumand, A, Ziaei-Nejad, S., 2016b. The effect of probiotic Bacillus subtilis bacteria extracted from the intestine of common carp (Cyprinus carpio) on growth performance and survival of the fish. Journal of Aquaculture Development 10(2),108-99. DOI: 20.1001.1.23223545.1395.10.2.9.1
Mazurkiewicz, J., Przybyl, A., Golski, j. , 2008. Evaluation selected feeds differing in dietary lipids levels in feeding juveniles of Wells catfish (Silurus glanis). Acta Ichthyologica et Piscatoria 38, 91-96. DOI: 10.3750/AIP2008.38.2.02
Merrifield, D.L., Bradley, G., Baker, R.T.M., Davies, S.J., 2010. Probiotic applications for rainbow trout (Oncorhynchus mykiss Walbaum) II. Effects on growth performance, feed utilization, intestinal microbiota and related health criteria postantibiotic treatment, Aquaculture Nutrition 16, 496–503. DOI: 10.1111/j.1365-2095.2009.00688.x
Nikoskelainen, S., Ouwehand, A.C., Bylund, G., Salminen, S., Lilius, E.M., 2003. Immune enhancement in rainbow trout (Onchorhynchus mykiss) by potential probiotic bacteria (Lactobacillus rhamnosus). Fish. & Shellfish Immunology 15, 443-452. DOI: 10.1016/s1050-4648(03)00023-8.
Pour Mohammadali Habibi, M., Rahimi Afzal, Z., Mohammadalikhani, M., 2024. Investigating the effect of the use of PrimaLac probiotics on the factors common carp blood serum. Caspian Journal of Veterinary Sciences 1(1), 29-35. DOI: 10.22034/cjvs.2024.198846
Ramos, M.A., Batista, S., Pires, M.A., Silva, A.P., Pereira, L.F., Saavedra, M.J., Ozório, R.O.A., Rema, P., 2017. Dietary probiotic supplementation improves growth and the intestinal morphology of Nile tilapia. Animal 11(8), 1259-1269. DOI: 10.1017/S1751731116002792
Suzer, C., Çoban, D., OkanKamaci, H., Saka, Ş., Firat, K., Otgucuoğlu, Ö., and Küçüksari, H., 2008. Lactobacillus spp. bacteria as probiotics in gilthead sea bream (Sparusaurata, L.) larvae: Effects on growth performance and digestive enzyme activities. Aquaculture 280, 140-145. DOI: 10.1016/j. aquaculture.2008.04.020
Thongprajukaew, K., Kovitvadhi, U., Kovitvadhi, S., Somsueb, P., Rungruangsak-Torrissen K., 2011. Effects of different modified diets on growth, digestive enzyme activities and muscle compositions in juvenile Siamese fighting fish (Betta splendens Regan, 1910). Aquaculture 322, 1-9. DOI: 10.1016/j.aquaculture.2011.10.006
Velisek, J., Svobodoza, Z., Piaakova, V., 2005. Effects of clove oil anesthesia on rainbow trout (Oncorhynchus mykiss). Acta Veterinaria Brno 74, 139 -146. DOI: 10.2754/avb200574010139
Wang, Y.B., Xu, Z., 2006. Effect of probiotics for common carp (Cyprinus carpio) based on growth performance and digestive enzyme activities. Animal Feed Science and Technology 127, 283-292. DOI: 10.1016/j.anifeedsci.2005.09.003
Wang, Y.B., Tian, Z., Yao, J., Li, W., 2008. Effect of probiotics, Enteroccus faecium, on Tilapia (Oreochromis niloticus) growth performance and immune response. Aquaculture, 277, 203-207. DOI: 10.1016/j.aquaculture.2008.03.007
Zhang, H., Wang, H., Hu, K., Jiao, L., Zhao, M., Yang, X., Xia, L., 2019. Effect of dietary supplementation of Lactobacillus casei YYL3 and L. plantarum YYL5 on growth, immune response and intestinal microbiota in channel catfish, Animals (Basel) 9,1005-1020. DOI: 10.3390/ani9121005
Ziaei-nejad, S., Rezaei, M.H., Takami, G.A., Lovett, D.L., Mirvaghefi, A.R., Shakouri, M., 2006. The effect of Bacillus spp. bacteria used as probiotics on digestive enzyme activity, survival and growth in the Indian white shrimp (Fenneropenaeus indicus). Aquaculture 252, 516-524. DOI: 10.1016/j.aquaculture.2005.07.021
Ziaei-nejad, S., 2016. Application of probiotics in modern aquaculture. Iranian fisheries science research institute. Tehran,Iran. 172 p.
Ziaei-nejad, S., Sharifpour, I., 2016. The effects of two synbiotics on biochemical and immunological parameters of White shrimp (Litopenaeus vannamei) hemolymph. Iranian Scientific Fisheries Journal 24(4),169-177. DOI: 10.22092/ISFJ.2017.110215
Ziaei-nejad, S., Abaei, N.K., Doost, B.N., Johari S.A., 2021. Effects of Supplemental Feeding of Common Carp (Cyprinus carpio) with Iron Nanoparticles and Probiotic Lactobacillus on Blood Biochemical Factors. Biology Bulletin of the Academy of Science 48, 177–184. DOI:10.1134/S1062359021020163

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