A study on the effect of temperature and pH on the lipase activity purified from the foregut of Rainbow trout (Oncorhynchus mykiss)

Document Type : Research Paper

Authors

1 Department of Fisheries, Faculty of Natural Resources, Tehran University, Karaj, Iran

2 Department of Biotechnology, Razi Vaccine & Serum Research Institute, Karaj, Iran

3 Department of Fisheries, Faculty of Natural Resources and Environment, Malayer University, Malayer, Iran

Abstract

Lipases are one of the most important digestive enzymes that have considerable physiological significance and industrial potential. Aquatics are one of the important resources for enzymes purification such as lipase. In this study, effect of temperature and pH on the lipase activity purified from the foregut of Rainbow trout, was assessed. For the purification, ammonium sulphate for the precipitation and ultrafiltration for concentration of sample was used. Enzyme activity was assessed by Nitrophenyl palmitate as substrate in different temperatures and pH to determination the best of temperature and pH for enzyme activity. The results showed that the optimum temperature and pH for rainbow trout lipase were 40°C and 8, respectively. Thermal and pH stability were also 4-40°C and 6-8 for rainbow trout lipase activity.

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