Investigate the possibility of Sex determination of rainbow trout (Oncorhynchus mykiss) using molecular markers

Document Type : Research Paper


1 Associate Professor, Department of Fisheries, Faculty of Natural Resources, University of Tehran, Tehran, Iran

2 MSC in Fisheries Engineering, Department of Fisheries, Faculty of Natural Resources, University of Tehran, Tehran, Iran

3 Associate prof. Department of Fisheries, Faculty of Natural Resources, University of Tehran, Tehran, Iran


The study investigates the possibility of sex determination of rainbow trout (Oncorhynchus mykiss)
using molecular screen for products monosex of populations. 35 caudal fin tissue samples were
obtained from Karaj Rainbow trout mature population and then genomic DNA was extracted and
marker loci amplified. Polymerase chain reaction (PCR) was setup based on two omyFA and
OmyFATU markers for amplification of sex linked markers in rainbow trout. Finally, the population
was screened based on presence of a specific band for both markers. Result of this expriments showed
that presence of omyFA and OmyFATU markers in males were %100 and %55 respectively. Acording
to the result omyFATU is proper marker for screen of karaj trout population.


Main Subjects

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