The effects of Bacillus subtilis and Lactobacillus plantarum bacteria on survival, growth and digestive tract microflora of Achanthopagrus latus larvae with different delivery methods

Document Type : Research Paper

Authors

1 Assistant Professor, Department of Fisheries, Natural Resources Faculty, Behbahan Khatam Alanbia University of Technology, Behbahan, Iran

2 Professor, Department of Fisheries, Natural Resources Faculty, University of Tehran, Karaj, Iran

3 Associate Professor, Department of Fisheries, Natural Resources Faculty, University of Tehran, Karaj, Iran

Abstract

This study examined the effects of two bacteria (Bacillu subtilis BP6 and Lactobacillus plantarum LP3) on survival, growth and digestive tract microflora of Achanthopagrus latus larvae with different delivery methods. For each bacterium was considered three treatments with different delivery methods. In first treatment, fish larvae were exposed to each bacterium through feeding them with bacteria-enriched Artemia. In second and third treatment, fish larvae were exposed to each bacterium through adding it directly to the water at 103 and 106 cells/ml respectively. The results show that in fish larvae to which bacteria had been administered, exhibited significant (P<0.05) increases in both survival and growth parameters wet weight as compared to control. Both bacteria could colonize the water and the gastrointestinal tract with relatively consistent trend.  

Keywords

Main Subjects


Alizadeh M., Farzanfar  A., Nafisi-Bahabadi M., 2011The effect of probiotic Bioplus 2B on growth performance and carcass composition of rainbow trout, Oncorhynchus mykiss (Walbaum, 1792) larvae ., Indian J. Fish., 58(4) : 55-59
 
Avella MA, Olivotto I, Silvi S, Place AR, Carnevali O., 2010. Effect of dietary probiotics on clownfish: a molecular approach to define how lactic acid bacteria modulate development in a marine fish. Am J Physiol Regul Integr Comp Physiol. 2010 Feb; 298(2):R359-71.
 
Balcázar, J.L.,  Vendrell, D., Blas, I.d., Ruiz-Zarzuela, I.,  Muzquiz, J.L., Girones, O., 2008. Characterization of probiotic properties of lactic acid bacteria isolated from intestinal microbiota of fish., Aquaculture, 278, 188-191.
 
Carnevali, O.; Zamponi, M. C.; Sulpizi, R.; Rollo, A.; Nardi, M.; Orpianesi, C.; Silvi, S.; Caggiano, M.; Polzonetti, A. M.; Cresci, A., 2004. Administration of probiotic strain to improve sea bream wellness during development. Aquaculture International, v. 12, n. 4-5, p. 377-386,
 
Carnevali, O., Vivo, L., Sulpizio, R., Gioacchini, G., Olivotto, L.,  Silvi S., and Cresci, A., 2006. Growth improvement by probiotic in European sea bass juveniles (Dicentrarchus labrax, L.), with particular attention to IGF-1, myostatin and cortisol gene expression, Aquaculture 258, 430–438.
 
Dalmin, G.,  Kathiresan K.,  and Purushothaman, A., 2001. Effect of probiotics on bacterial population and health status of shrimp in culture pond ecosystem, Indian J. Exp. Biol. 39, 939–942.
 
Faramarzi M., Jafaryan H., Patimar R., Iranshahi F., Lashkar Boloki M., Farahi A., Kiaalvandi S., Ghamsary M., Makhtoumi and N.M., The Effects of Different Concentrations of  Probiotic Bacillus spp. And Different Bioencapsulation Times on Growth Performance and Survival Rate of Persian Sturgeon (Acipencer persicus) Larvae., World Journal of Fish and Marine Sciences 3 (2): 145-150
 
Fuller, R., 1992. History and development of probiotics. In: Fuller, R.( Ed.) , Probiotics: the Scientific Basis. Chapman & Hall, New York, pp. 1–8.
 
Gatesoupe, F.J., 1999. The use of probiotics in aquaculture. Aquaculture 180, 147-165.
 
Gildberg, A., Mikkelsen, H., Sandaker, E., Ringo, E., 1997. Probiotic effect of lactic acid bacteria in the feed on growth and survival of fry of Atlantic cod (Gadus morhua ). Hydrobiologia 352, 279–285.
 
Gomez-Gil, B., Roque, A. and Velasco-Blanco, G. 2002. Culture of Vibrio alginolyticus C7b, a potential probiotic bacterium, with the microalga Chaetoceros muelleri. Aquaculture 211, 43-48.
 
Huys, L., Dhert, P., Robles, R., Ollevier, F., Sorgeloos, P., Swings J., 2001. Search for beneficial bacterial strains for turbot (Scophthalmus maximus L.) larviculture Aquaculture, 193, 25-37.
 
Itami, T., Asano, M., Tokushige, K., Kubono, K., Nakagawa, A., Takeno, N., Nishimura, H., Maeda, M.,Kondo, M., Takahashi, Y., 1998. Enhancement of disease resistance of Kuruma shrimp, Penaeus japonicus, after oral administration of peptidoglycan derived from Bifidobacterium thermophilum. Aquaculture 164, 277–288.
 
Kennedy, S.B., Tucker, J.W., Thoresen, M., Sennett, D.G., 1998. Current methodology for the use of probiotic bacteria in the culture of marine fish larvae. Aquaculture 98. World Aquaculture Society, Baton Rouge, p. 286.
 
Lara-Flores, M., M.A. Olvera-Novoa, B.E. Guzman-Mendez, and W. Lopez-Madrid. 2003.  Use of the bacteria Streptococcus faecium and Lactobacillus acidophilus, and the yeast Saccharomyces cerevisiae as growth promoters in Nile tilapia (Oreochromis niloticus).  Aquaculture 216: 193-201.
 
Mohamed A.H., Traifalgar R.F.M., Serrano A.E., 2013  Assessment of Probiotic Application on Natural Food, Water Quality and Growth Performance of Saline Tilapia Oreochromis mossambicus L. Cultured in Concrete Tanks, Fisheries and Aquaculture Journal, Vol. 2013: FAJ-75
 
Moriarty, D.J.W., 1998. Control of luminous Vibrio species in penaeid aquaculture ponds. Aquaculture 164,351–358.
 
Nayak, S.K., Swain, P., Mukherjee, S.C., 2007. Effect of dietary supplementation of probiotic and vitamin C on the immune response of Indian major carp, Labeo rohita (Ham.) Fish & Shellfish Immunology, 23, 892-896.
 
Olafsen, J.A., 2001. Interactions between fish larvae and bacteria in marine aquaculture. Aquaculture, 200, 223-247.
 
Rengpipat, S., Rukpratanporn, S., Piyatiratitivorakul, S., Menasaveta, P., 2000. Immunity enhancement on black tiger shrimp (Penaeus monodon) by a probiont bacterium (Bacillus S11). Aquaculture 191,271-288.
 
Ringo E., Vadstein, O., 1998. Colonization of Vibrio pelagius and Aeromonas caviae in early developing turbot, Scophtalmus maximus( L.) larvae. J. Appl. Microbiol. 84, 227–233.
 
Riquelme, C., Araya, R., Escribano, R., 2000. Selective incorporation of bacteria by Argopecten purpuratus larvae: implications for the use of probiotics in culturing systems of the Chilean scallop. Aquaculture 181, 25–36.
 
Strøm, E. and Ringø, E. 1993. Changes in the bacterial composition of early developing cod, Gadus morhua (L.) larvae following inoculation of Lactobacillus plantarum into the water. In Physiology and Biochemical Aspects of Fish Development. Walther,B.T. and Fyhn,H.J. (eds.). University of Bergen, Bergen, Norway p. 226-228.
 
Suzer, C.,  Coban, D., Kamaci, O.H., Saka, S., Firat,  K., Otgucuoglu  Ö.,  and Kücüksari, H., 2008.  Lactobacillus spp. bacteria as probiotics in gilthead sea bream (Sparus aurata, L.) larvae: Effects on growth performance and digestive enzyme activities. Aquaculture, 280, 140–145.
 
Tannock, G. W. 1999. Identification of Lactobacilli and Bifidobacteria. Current Issues Molec. Biol. 1(1): 53-64.
 
Wang, Y.B., 2007. Effect of probiotics on growth performance and digestive enzyme activity of the shrimp Penaeus vannamei, Aquaculture 269, 259–264.
 
Ziaei-Nejad, S., Habibi-Rezaei, M., Azari-Takami G., Lovett, DL., Mirvaghefi, A. & Shakouri, M., 2006. The effect of Bacillus spp. bacteria used as probiotics on digestive enzyme activity, survival and growth in the Indian white shrimp Fenneropenaeus indicus. Aquaculture 252, 516-524.
 
Ziaei-Nejad, S., 2010. Screening of probiotic bacteria from yellowfin seabream (Acanthopagrus latus) gastrointestinal tract and investigating their effects on growth and survival indices of the fish. PhD thesis. Tehran University