Nutritional Effects of Black Powder Seeds (Nigella sativa) on Antibacterial Activity and Safety Mucus Indicators Caspian Roach (Rutilus caspicus)

Document Type : Research Paper

Authors

1 M.Sc. Graduate, Department of Fisheries, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.

2 Professor, Department of Fisheries, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.

3 Ph.D Student, Department of Fisheries, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.

Abstract

In this study, the effect different levels of Nigella sativa feed on mucus, antibacterial activity of some enzymes’ mucus were examined the baby fish roach (‌Rutilus caspicus). 420 carp with average weight of 4/49 ± 0/11 gr on 4 group‌ control , 0.5, 1 and 2 percent seed powder per kg of diet were fed with three replications for 60 days . At the end of levels of soluble protein mucus, antibacterial activity and phosphatase alkaline by microdilution methods were measured against Gram-positive bacteria aureus Staphylococcus, Streptococcus faecalis and Gram-negative bacteria Escherchia coli and Pseudomonas aeruginosa. The showed  results that the antibacterial activity of mucus with increasing levels of black seed increased and the diameter of the growth in fish fed  with %2 black seed significantly increased compared with the control group(P<0/05) and  the most diameter of inhibition against was observed to the bacteria Streptococcus faecalis. Also the levels soluble protein of mucus, lysozyme enzyme and phosphatase alkaline in fish fed up Nigella sativa 0.5 Ratio to the control group significantly increased (P<0/05). Minimum the growth inhibitory concentration (MIC) on a concentration of 50 microliter on ml and concentration higher concentrations of mucus, was higher the antibacterial activity and the concentration 200 microlitre per ml the most was seen antibacterial activity. Overall results that showed   diet of black seed has a positive effect on the antibacterial activity is the mucus roach fish.

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Journal of Fisheries
Volume 69, Issue 4
March 2017
Pages 443-451

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