Effect of Chitosan Nanocomposites and Rosemary Extract (Rosmarinus officinalis L.) Coating on Chemical Properties of Inoculated Fillet of Huso huso with Listeria monocytogenes during Refrigerated Storage

JafariKaliji, Alika; Jafarpour, Ali; Safari, Reza

doi:10.22059/jfisheries.2018.207265.929

Effect of Chitosan Nanocomposites and Rosemary Extract (Rosmarinus officinalis L.) Coating on Chemical Properties of Inoculated Fillet of Huso huso with Listeria monocytogenes during Refrigerated Storage

Document Type : Research Paper

Authors

Alika JafariKaliji ¹

Ali Jafarpour ²

Reza Safari ³

¹ M.Sc. student, Department of Fisheries, Faculty of Animal Science and Fisheries, Sari Agriculture Science and Natural Resources University (SANRU), Sari, Iran.

² Associate professor, Department of Fisheries, Faculty of Animal Science and Fisheries, Sari Agriculture Science and Natural Resources University (SANRU), Sari, Iran.

³ Senior Researcher, Caspian Sea Ecology Research Center, Sari, Iran

10.22059/jfisheries.2018.207265.929

Abstract

This study aimed to evaluate the antioxidative and antimicrobial effects of chitosan nanocompsites and rosemary extract coating in the inoculated fillet of Huso huso with Listeria monocytogenes during refrigerated storage (4±1ºC). Fish were filleted and divided into four groups as control (without coating), 0.5% rosemary extract treatment (RET), 1% chitosan treatment (CT) and combination of 1% chitosan and 0.5% rosemary extract as chitosan nanocomposites treatment (CS/RE). Then all samples inoculated with L. monocytogenes. Subsequently, the chemical parameters (TVB-N, PV, pH and TBA) and antilisterial properties of coatings were monitored during 16-days of refrigerating storage at 4±1ºC. According to the results, CS/RE demonstrated a significant (P<0.05) ability to inhibit the growth of L. monocytogenes from 4.14 log cfu/g to 2.23 log cfu/g at the end of the storage period, followed by CT and RET treatments, respectively, compared to the control. In terms of chemical parameters, even though samples coated with CS/RE had the lowest pH, and TVB-N values (P<0.05), however, this coating was not able to pause the protein denaturation after 8 days of storage (P>0.05) compared to the other treatments. On the other hand, CS/RE coating retarded lipid oxidation by decreasing PV and TBA production in the samples compared to the control up to the end of refrigerated storage (P<0.05).

Keywords

Chitosan nanocomposite

Rosemary extract

Chitosan

Listeria monocytogenes

Huso huso

Subjects