Determining  of  Copper Oxide Nanoparticles (CuO NPs)  LC50 in Grass Carp  (Ctenopharyngodon idella) and   Its Effects  on  Hematological  and  Liver  Enzymes Activity  Indices

Vafadarnejad, Masoomeh; Gharaei, Ahmad; Mirdar Harijani, Javad; Miri, Mohadeseh

doi:10.22059/jfisheries.2018.254193.1024

Determining of Copper Oxide Nanoparticles (CuO NPs) LC50 in Grass Carp (Ctenopharyngodon idella) and Its Effects on Hematological and Liver Enzymes Activity Indices

Document Type : Research Paper

Authors

Masoomeh Vafadarnejad ¹

Ahmad Gharaei ²

Javad Mirdar Harijani ³

mohadeseh miri ⁴

¹ M.Sc. Student, Department of Fisheries, Faculty of Natural Resources, University of Zabol, Zabol, Iran

² Associate Professor, Departments of Fisheries, Hamoun International Wetland Research Institute and Faculty of Natural Resources, University of Zabol, Zabol, Iran.

³ Assistant Professor, Department of Fisheries, Faculty of Natural Resources, University of Zabol, Zabol, Iran.

⁴ Instructor, Departments of Fisheries, Hamoun International Wetland Research Institute and Faculty of Natural Resources, University of Zabol, Zabol, Iran.

10.22059/jfisheries.2018.254193.1024

Abstract

In this study, the effects of different levels of copper oxide nanoparticles (CuO NP_S) were evaluated on hematological indices and liver enzymes activity of the grass carp (Ctenopharyngodon idella). Firstly, 150 fish (with mean weigh 38± 5 g) exposed under different concentrations of copper oxide nanoparticles for 96 hours and were recorded daily losses. As a result, the concentration of lethal toxicity (LC₅₀) nano-copper oxide for grass carp was obtained 2589.14±0.5 mg/l. Then the fish exposed under sub-lethal concentrations (1/20, 1/30 and 1/50 concentration LC₅₀) copper oxide nanoparticles for 10 days that were measured hematological indices and liver enzyme activities in fish. The results showed that the nano-particles causes changes in the blood parameters of the fish that these changes was affected by the nano-particles in blood indices by reducing the level of red blood cells (RBC), Hematocrit and increase in white blood cells (WBC) and liver enzymes in treatment groups (p<0.05). Finally, the results suggested that copper nanoparticles in the high level could side effect on the fish health. Therefore, preventing the entry of these new materials into the food chain of aquatic organisms seems to be necessary.

Keywords

Copper oxide nanoparticle

Hematocrit

Liver enzymes activity

Sub lethal toxicity

Subjects