The Antibacterial activity of ß amberine extracted from the gonad of sea cucumber Holothuria leucospilota inhabiting Hengam island, Persian Gulf

Document Type : Research Paper

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Abstract

Owing to the antibacterial activities of sea cucumber and the abundance of Holoturia locuspila in the Persian Gulf, the antibacterial activity of ß amberine extreacted from the gonad of this sea cucumber species was studied. In this investigation, acetone extract was prepared from the dried powder of sea cucumber. Isolation of ß amberine was carried out by column chromatography on silica gel and identification was carried out by TLC and GC/MS. In vitro antibacterial screening was determined by broth dilution against bacteria; Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumonia, Salmonella typhi, Proteus vulgaris, Staphylococcus aureus, Bacillus cereus, Bacillus subtilis and Nocardia brasiliensis. The ß amberine formula C30H48O was detected by GC/MS from no. 11 fraction with 98% purity. This component showed considerable activity against Bacillus cereus, Bacillus subtilis (MBC = 1000 µg/mL) and Salmonella typhi, Staphylococcus aureus (MBC = 2000 µg/mL). According to our results, ß amberine extracted from sea cucumber can be considered as a source of novel antibacterial drug.

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Journal of Fisheries
Volume 72, Issue 2
September 2019
Pages 211-220

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