Study of superoxide dismutase (SOD), an antioxidant enzyme, changes in the liver of Rinbow trout (Onchorhyncus mykiss) treated by oxytetracycline

Document Type : Research Paper


1 Fishery

2 University of Tehran


This study was conducted to investigating the effect of dietary supplemented by butylated hydroxytoluen (BHT), a synthetic antioxidant, and oxytetracycline (OTC) on superoxide dismutase (SOD) on content of the liver in rainbow trout(Onchorhyncus mykiss) experimentally exposed Aed against Aeromonas hydrophila. Fish were divided into experimental treatments in triplicates and received the following experimental diets, after bacterial exposure ( CFU/ml through peritonile injection), for 2 days:(E)-control; (B)- OTC,; (C)- OTC plus dietary 0/5 % BHT; (D)- OTC plus dietary 0/8 % BHT(9 g OTC, effective dosage 20%, was administrated in diets for three treatments as mentioned above, B, C and D). After fish liver sampling, OTC was omitted from(C) and (D) treatments and experiment was continued by: (C1)- dietary 0/5 % BHT (D1)- dietary 0/8 percent BHT, for 7 days and final sampling was applied. Statistical analysis in SAS 9.2 software showed that using the dietary supplemented by OTC and 0/5 % BHT during antibiotic treatment as well as using dietary supplemented by 0/8 % BHT in following antibiotic treatment can induce of SOD increase in the liver of fish.