The present study aimed to investigate the effect of R. canina L hydroalc extract on growth and nutrition factors and immune indicators of rainbow trout (oncorhynchus mykiss). In this study, 230 fish with weight average of 15±0.7 were randomly assigned in four groups and were studies for 42 days with three replications. The experimental treatments including R. canina L-free diet as the control treatment and treatments including 2.5, 5, and 10 (g/kg) R. canina were the experimental treatments. Fish were fed by 2% of their body weight twice a day and after two weeks, they were weighted and different growth and immune factors were examined. According to the findings of the growth indicators, the treatment containing 5 )g/kg( R.canina significantly showed better )FCR(, )SRG(, )CF(, )BWG( and )DGR( indicators (P˂0.05). The results of measuring hematological indicators of )Hb(, )RBC(, )MCV(, )MCH(, and )MCHC( showed no significant difference between the treatments (p˃0.05). WBC showed a significant difference between treatments containing extract (P˂0.05) and the treatment 10) g/kg( showed no difference with the control treatment (P˃0.05).The number of lymphocytes in the treatment group10(g/kg) showed a significant difference with other treatments and the control treatment. No significant difference was observed between the treatments in terms of neutrophils and monocytes. The findings of this study show that the use of 5 )g/kg( extract leads to increased growth indicators and 10 )g/kg(leads to increased growth indicators.