Document Type : Research Paper
Authors
1
Department of Fisheries, University of Zabol, Zabol, Sistan and baluchestan, Iran
2
Department of Fisheries, Faculty of Natural Resources, University of Zabol, Zabol, Iran.
3
Department of Food Hygiene and Aquatic Animals, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran
4
departement of environmental science, faculty of natural resorces, university of zabol
Abstract
Today, the replacement of plant extracts instead of chemical preservatives in the diet has been considered with reducing the adverse effects of chemical preservatives. In this study, the effects of black grape seed extract on growth, intestinal and liver histology, as well as gastrointestinal enzymes, in common carp were investigated. Grape seed extract (30g kg-1) was added to the basal diet and at the end of the experimental period, the digestive enzymes, including lipase, amylase, and trypsin, were measured. The fish were also dissected, then intestine and liver were collected and fixed for histological studies. The results showed that among different digestive enzymes, only lipase had a significant increase (1.67±0.10 U/mg protein) compared to control group (1.35±0.04 U/mg protein). In the intestinal and liver histology, supplemental diet did not have any side effects on the normal structure. The ratio of villus height to the distance between two adjacent villus in the basal part was slightly higher in treated fish (17.39 ± 0.5μm) than the control group (16.71 ± 0.59 μm), but no significant differences was observed. Also, the number of goblet cells in control group (4.59±0.17μm) was slightly higher than treated fish (4.09±0.21μm), however, no significant difference was observed. Morphometric study in liver also showed an increase in hepatocyte size (24.01±0.16µm) compared to the control group (23.42±0.14µm). In general, the increase of the digestive enzyme activity and healthy liver and intestine structure could show the potential of grape seed extract as a beneficial feed additive in common carp.
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