Document Type : Research Paper
Authors
1
1. Assistant Professor, Persian Gulf and Oman Sea Ecological Cener, Iranian Fisheries Science Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Bandar Abbas, Iran.
2
, Iranian Fisheries Science Research Institute, Agricultural Research, Education and Extension Organization, Tehran, Iran
3
4- Persian Gulf & Oman Sea Ecological Research Institute, Iranian Fisheries Science Research Institute, Agricultural Research, Education and Extension Organization, Bandar Abbas, Iran.
4
Assistant Professor, Persian Gulf and Oman Sea Ecological Cener, Iranian Fisheries Science Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Bandar Abbas, Iran
Abstract
Due to exist of the different kinds of sea cucumbers and their biological activities, In this study, the antibacterial activities and the soap forming ability of powder, methanolic and saponins extracts of sea cucumber (Holothuria leucospilota). obtained from Lavan Island, were evaluated. Initially Sea cucumbers were dried and subsequently ground. Extraction was performed by methanol solvents to obtain total extract and 70% ethanol. The method of soaking was used in order to prepare the extract containing the ultimate amount of saponin compounds. With the aim of determine the quality of saponin, its index of foam forming ability was evaluated. The production of soap from marine cucumbers was carried out using the method of soaping fats and glycerin. Finally, sea cucumber soaps was produced using powder, methanolic and saponins extracts with ratios of 1, 5 and 10%. In order to fulfill of bacterial analysis, the effect of extracts and prepared soaps on Staphylococcus aureus was evaluated using tubular dilution method. The results of the experiment show that the minimum inhibitory concentration (MIC) of the methanolic extract and marine cucumber soap for Staphylococcus aureus is 10 and 5 mg / ml respectively. This index for saponin and its soap is 2 and 5 mg / ml. Regardless of the duration of the experiment, one-way analysis of variance test showed that there was a significant difference in the mean height between volumes of 10 ml and volumes of one, two, three, four and five ml (p <0.05). In other cases, no significant difference was observed in the mean floor height. The produced foam of the sea cucumber saponin remained for more than 12 hours, indicating a very high foam formin ability. In this project, the antibacterial and consequently anti- pimple of extracts and soaps produced from the Holothuria leucospilotaobtained from Lavan Island were studied and confirmed. Therefore, this compounds can be used as an appropriate choice for the production of anti-pimple soaps.
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