Effects of media containing glycerol and glucose on carbohydrates, lipids and protein production and antioxidant activity in Scenedesmus and Monoraphidium

Document Type : Research Paper

Authors

1 Associate Professor, Biotechnology Department, Faculty of Biological Sciences, Alzahra University, Tehran, Iran

2 M.Sc. Graduated student, Biotechnology Department., Faculty of Biological Sciences, Alzahra University, Tehran, Iran

3 Assistant Professor, Biotechnology Department, Faculty of Biological Sciences, Alzahra University, Tehran, Iran

Abstract

Microalgae are a very diverse group of aquatic plants that have numerous applications in biotechnology today. These microscopic photosynthesizers, have an extensive role in producing oxygen on Earth, they are found in almost all water sources on Earth due to their high dispersion and abundance. In this study, to investigate the effect of carbon sources on biochemical compounds such as proteins, carbohydrates and antioxidants, sources of organic carbon like glucose and glycerol were used. The results showed that most carbohydrates production was in a sample with 3 g / liter of glucose plus 5 g / Liters of glycerol in Monoraphidium and the lowest amount in the control sample of Scenedesmus. The lowest amount of protein was in the sample contains 1 g / liter of glucose in Scenedesmus and the highest amount in the control sample of Monoraphidium. The amount of antioxidants calculated by FRAP, and it was the highest in the control sample of Monoraphidium and the lowest recorded in the sample containing 3 g / liter of glucose in Monoraphidium. The results of this study indicated that the use organic carbon sources such as a combination of glucose and glycerol stimulates the production of carbohydrates but reduces the production of proteins and antioxidants.

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