Document Type : Research Paper
Authors
1
MSc graduate, Department of Fisheries, Faculty of Natural Resources, University of Tehran, Karaj, Iran.
2
Professor, Department of Fisheries, Faculty of Natural Resources, University of Tehran, Karaj, Iran.
3
Assistant Professor, Department of Fisheries, Faculty of Natural Resources, University of Tehran, Karaj, Iran.
4
BSc graduate, Shahid Ansari restocking and protection of teleosties genetic resources center, Rasht, Iran.
Abstract
Southern Caspian Kutum, Rutilus frisii kutum (Kamensky, 1901) is an important economic species in north of Iran. This study was conducted in order to identifying QTLs associated with growth traits, body weight and total length, in Caspian Kutum (R. frisii kutum) using microsatellites from close species, Common Carp (cyprinus carpio). The eleven full-sibs family were produced by mating between eleven male and eleven female that were collected from Sefid Rood river. Sampling was accomplished from broodstock caudal fin. Three month after hatch the offspring were collected and stored in alcohol 96%. Altogether 75 offspring were sampled. Genomic DNA was isolated from broodstock and offspring according to the protocol. With applying four microsatellite markers (HLJ2225, HLJ3366, HLJ3988, and HLJ2316) related to growth traits, altogether 10 loci, 13 genotype, were selected for performing statistical analyses. Genotyping was accomplished using AlphaEaseFC software. Statistical analyses were performed by GenAlEx 6.1, Arlequin ver. 3.5.2 and Excel 2013 softwares. Investigating the number of alleles per locus, effective number of alleles per locus, expected heterozygosity, observed heterozygosity, fixation index, polymorphic information content, R and F statistics, Hardy-Weinberg equilibrium showed that these loci, HL1, HL2, HL3, HL4, HL5, HL6, HL7, HL8, HL9 and HL10, were suitable for applying in marker-assisted-selection (MAS) program for Caspian Kutum (R. frisii kutum). The heritability was calculated for each locus, for total length and total weight. Then based on linkage disequilibrium five heritable linkage group, LG1, LG2, LG3, LG4 and LG5, were constructed for total length in Caspian Kutum (R. frisii kutum). The LG4 with eight loci (nine genotype), including loci HL3, HL4, HL5, HL6, HL7, HL8, HL9 and HL10, was selected for family linkage group selection (LGS). The LG4 had a relatively average heritability ( = 0/432). Altogether, six family based on LG4 were selected to use in R. frisii kutum selective breeding program. This study showed that using microsatellite markers related to growth traits in close species to Caspian Kutum (R. frisii kutum) can aid to identifying QTLs associated with growth traits in Caspian Kutum, an important commercial species.
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