Document Type : Research Paper
Authors
1
PhD student in biochemistry, Department of Biology, University of Isfahan, Isfahan, Iran. Email: jafarishahrbanoo@gmail.com
2
Assistant Professor, Department of Biology, Payame Noor University, Tehran, Iran.(Author). Email: ra_rahbarian@pnu.ac.ir
3
Assistant Professor, Department of Biology, Payame Noor University, Tehran, Iran. Email: rajabian.m@pnu.ac.ir
4
Associate Professor, Department of Biology, Payame Noor University, Tehran, Iran. Email: hramshini@yahoo.com
5
MSc, Department of Biology, Payame Noor University, Tehran, Iran. Email: jafariamir1357@yahoo.com
Abstract
This study was performed to investigate the effect of increasing pomegranate peel extract in the diet of common carp on inhibiting the oxidative damage from experimental cadmium poisoning. In this experiment, fish weight with an average of 70 grams were randomly divided into the five experimental groups. Experimental unite was an aquarium with the capacity of holding twenty120-liter of water. The first group was considered as control. The second group received cadmium chloride, while the third group was treated with cadmium chloride and pomegranate peel extract (1% of dietary weight), the fourth group received cadmium chloride and pomegranate peel extract (2 % of diet weight) and the fifth group received cadmium chloride and pomegranate peel extract (4% of diet weight) for a 30- day culture period. At the end of the test period, hepatic markers of alanine, aminotransferase, aspartate, aminotransferase, and alkaline phosphatase and catalase were measured in the serum. The results showed that the amount of the above enzymes in the groups treated with cadmium chloride increased significantly compared to the control group (p <0.05). Also, the addition of increasing concentrations (1, 2, and 4%) of pomegranate extract significantly reduced all liver parameters compared to the group treated with cadmium chloride (p <0.05). The catalase enzyme was increased in the cadmium-treated group compared with the control. Based on the findings, it can be concluded that increasing pomegranate peel extract in the diet of common carp can have modulatory effects on biochemical changes in liver enzymes and catalase.
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