Extraction of phycocyanin from Arthrospira platensis with different solvents and methods

Document Type : Research Paper

Authors

1 MSc Graduated, Department of Fisheries, Faculty of Natural Resources, University of Tehran, Karaj, Iran

2 Professor, Department of Fisheries, Faculty of Natural Resources, University of Tehran, Karaj, Iran

3 Associate Deprtment of Fisheries, Faculty of Natural Resources, University of Tehran

Abstract

In this study, phycocyanin pigment extraction from Arthrospira platensis microalgae was carried out using various solvents (distilled water, phosphate buffered saline) and methods (sonication, microwave and freezing & thawing) for comparison and determine the best solvent and method in order to achieve higher extraction yield. Rate of 0.1 g A. platensis powder in 10 cc of solvent (distilled water and phosphate buffered saline) was similar in each three extraction method whereas the conditions of extraction methods sonication, microwave and freezing & thawing as follows, respectively: 50% amplitude and 15 min, 100 W and 3 min, frozen at -20 ± 2 °C for 24 h and thawing at room temperature. The analysis of variance was obtained for data mean ± SD (standard deviation) using SPSS in confidence level of (P < 0.05). Most extraction yield was obtained by phosphate buffered saline (PBS) and method Freezing & Thawing (pc= 146/3 ± 1/55 mg/g) and least by distilled water and method Microwave (pc= 94/4 ± 2/68 mg/g).
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Keywords

References

Abalde, J., 1998. Purification and characterization of phycocyanin from the marine cyanobacterium Synechococcus sp. 109201. Plant Science 136(-), 109–120.
Antelo, F.S., Anschau, A., V Costa, J.A., Kalil, S.J., 2010. Extraction and purification of C-phycocyanin from Arthrospira platensis in conventional and integrated aqueous two-phase systems. Journal of the Brazilian Chemical Society 21(-), 921–926.
Arad, S. (Malis), Yaron, A., 1992. Natural pigments from red microalgae for use in foods and cosmetics. Trends in Food Science and Technology 3(-), 92–97.
Belay, A., 2002. The potential application of Spirulina (Arthrospira) as a nutritional and therapeutic supplement in health management. Journal of the American Nutraceutical Association 5(2), 26–49.
Bennett, A., Bogorad, L., 1973. Complementary chromatic adaption in a filamentous blue green alga. Journal of Cell Biology 58(-), 419–435.
Boussiba, S., & Richmond, A. E., 1980. C-phycocyanin as a storage protein in the blue green alga Arthrospira platensis. Archives of Microbiology 125(1–2), 143–147.
Chan, E.W.C., Lim, Y.Y., Wong, S.K., Lim, K.K., Tan, S.P., Lianto, F.S., Yong, M.Y., 2009. Effects of different drying methods on the antioxidant properties of leaves and tea of ginger species. Food Chemistry 113(-), 166–172.
Chan, E.W.C., Lye, P.Y., Eng, S.Y., Tan, Y.P., 2013. Antioxidant properties of herbs with enhancement effects of drying treatments: a synopsis. Free Radicals and Antioxidants 3(-), 2–6.
Chaiklahan, R., Chirasuwan, N., Loha, V., Tia, S., Bunnag, B., 2011. Separation and purification of phycocyanin from Arthrospira sp. using a membrane process. Bioresource Technology 102(-), 7159–7164.
Deng, R., Chow, T.-J., 2010. Hypolipidemic, antioxidant and antiinflammatory activities of microalgae Arthrospira. Cardiovascular Therapeutics 28(-), 33–45.
Dey, S., Rathod, V.K., 2013. Ultrasound assisted extraction of b-carotene from Arthrospira platensis. Ultrason Sonochem 20(-), 271-276.
Doke, J.M., 2005. An improved and efficient method for the extraction of phycocyanin from Arthrospira sp. International Journal of Food Engineering 1(-), 1–11.
Eriksen, N.T., 2008. Production of phycocyanin—a pigment with applications in biology, biotechnology, foods and medicine. Applied Microbiology and Biotechnology 80(-), 1–14.
Falkeborg, M.F., Roda-Serrat, M.C., Burnæs, K.L., Nielsen, A.L.D., 2018. Stabilizing phycocyanin by anionic micelles. Food Chemistry 239(-), 771–780.
Fontoura Prates, D., Radmann, E.M., Duarte, J.H., Morais, M.G., Vieira Costa, J.A., 2018. Arthrospira cultivated under different light emitting diodes: Enhanced cell growth and phycocyanin production. Bioresource Technology 256(-), 38–43.
Geciova, J., Bury, D., Jelen, P., 2002. Methods for disruption of microbial cells for potential use in the dairy industry – a review. International Dairy Journal 12(6), 541–553.
Ghosh, T., Paliwal, C., Maurya, R., Mishra, S., 2015. Microalgal Rainbow Colours for Nutraceutical and Pharmaceutical Applications. In: Bahadur, B., Venkat Rajam, M., Sahijram, L., Krishnamurthy, K. (Eds.), Plant Biology and Biotechnology. Springer, New Delhi, pp. 777–791.
Habib, M.A.B., Parvin, M., 2008. A review on culture, production and use of Arthrospira as food for humans and feeds for domestic animals and fish. FAO Fisheries and Aquaculture Circular, 1034.
Hao, S., Yan, Y., Huang, W., Gai, F., Wang, J., Liu, L., Wang, C., 2018. C-phycocyanin reduces inflammation by inhibiting NF-κ- activity through downregulating pdcd5 in lipopolysaccharide-induced raw 264.7 macrophages. Journal of Functional Foods 42(-), 21–29.
Ho, S.-H., Liao, J.-F., Chen, C.-Y., Chang, J.-S., 2018. Combining light strategies with recycled medium to enhance the economic feasibility of phycocyanin production with Arthrospira platensis. Bioresource Technology 247(-), 669–675.
Hosseini, S.M., Khosravi, K., Mozafari, M.R., 2013. Nutritional and medical applications of Spirulina microalgae. Mini Reviews in Medicinal Chemistry 13 (-), 1231–1237.
Hoseini S.M., Montazeri F., Afsharzadeh S. 2013. Comparison of cell disruption methods for extracting lipid from Spirulina platensis and Chlorella vulgaris. Journal of Agriculture Biotechnology 5(3), 45-56.
Ilter, I., Akyil, s., Demirel, Z., Koc, M., Conk­-­Dalay, M., Kaymak-Ertekin, F., 2018. Optimization of phycocyanin extraction from Arthrospira platensis. Journal of Food using different techniques composition and Analysis 70 (-), 78-88.
Jain, T., Jain, V., Pandey, R., Shukila, S.S., 2009. Microwave assisted extraction for phytoconstituents–an overview. Asian Journal of Research in Chemistry 2 (-), 19–25.
Jespersen, L., Strømdahl, L., Olsen, K., Skibsted, L., 2005. Heat and light stability of three natural blue colorants for use in confectionery and beverages. European Food Research and Technology 220(3), 261–266.
Jiang, L., Wang, Y., Yin, Q., Liu, G., Liu, H., Huang, Y., Li, B., 2017. Phycocyanin: A potential drug for cancer treatment. Journal of Cancer 8(17), 3416–3429.
Kannaujiya, V.K., Sinha, R.P., 2016. Thermokinetic stability of phycocyanin and phycoerythrin in food-grade preservatives. Journal of Applied Phycology 28(-), 1063–1070.
Kumar, D., Dhar, D.W., Pabbi, S., Kumar, N., Walia, S., 2014. Extraction and purification of C-phycocyanin from Arthrospira platensis (CCC540). Indian Journal of Plant Physiology 19(2), 184-188.
Manirafasha, E., Ndikubwimana, T., Zeng, X., Lu, Y., Jing, K., 2016. Phycobiliprotein: Potential microalgae derived pharmaceutical and biological reagent. Biochemical Engineering Journal 109(-), 282–296.
Martinez-Guerra, E., Gude, V.G., Mondala, A., Holmes, W., Hernandez, R., 2014. Microwave and ultrasound enhanced extractive-transesterification of algal lipids. Applied Energy 129(-), 354–363.
Medeiros, F.O., Alves, F.G., Lisboa, C.R., Martins, D.D.S., Burkert, C.A.V., Kalil, S.J., 2008. Ondas ultrassônicas e pérolas de vidro: um novo método de extração de b-galactosidase para uso em laboratório. Química Nova 31(2), 336–339.
Minkova, K.M., Tchernov, A.A., Tchorbadjieva, M.I., Fournadjieva, S.T., Antova, R.E., Busheva, M.C., 2003. Purification of C-phycocyanin from Arthrospira fusiformis. Journal of Biotechnology 102(-), 55–59.
Moraes, C.C., Burkert, J.F.D., Kalil, S.J., 2010. C-Phycocyanin extraction process for large-scale use. Journal of Food Biochemistry 34 (-), 133–148.
Moraes, C.C., Sala, L., Cerveira, G.P., Kalil, S. J., 2011. C-phycocyanin extraction from Arthrospira platensis wet biomass. Brazilian Journal of Chemical Engineering 28(1), 45-49.
Nakagawa, K., Ritcharoen, W., Sri-Uam, P., Pavasant, P., Adachi, S., 2016. Antioxidant properties of convective air-dried Arthrospira maxima: Evaluation of phycocyanin retention by a simple mathematical model of air-drying. Food and bioproducts processing 100(-), 292–302.
Newsome, A., Culver, C., Breemen, R., 2014. Nature’s palette: the search for natural blue colorants. Journal of Agricultural and Food Chemistry 62(28), 6498–6511.
Ores, J.C., Amarante, M.C.A., Kalil, S.J., 2016. Co-production of carbonic anhydrase and phycobiliproteins by Arthrospira sp. and Synechococcus nidulans. Bioresource Technology 219(-), 219-227.
Ores, J.C., Fernandes, S.S., Amarante, M.C.A., Silva, B.P.d., Kalil, S.J., 2013. Tecnologia enzimática para captura de CO2 cultivo de microalga para obtenção de anidrase carbônica. Vetor 23(2), 82-92.
Pan-Utai, W., Iamtham, S., 2018. Physical extraction and extrusion entrapment of C-Phycocyanin from Arthrospira platensis. Journal of king saud university- Science 31(-), 1535-1542.
Pan-utai, W., Iamtham, S., 2019. Extraction, purification and antioxidant activity of phycobiliprotein from Arthrospira platensis. Process Biochemistry 82(-), 189-198.
Park, W., Kim, H., Li, M., Lim, D., Kim, J., Kwak, S., M.G., Ferruzzi, Ahn, M., Kang, C.-M., 2018. Two classes of pigments, carotenoids and c-phycocyanin, in spirulina powder and their antioxidant activities. Molecules 23(8), 2065.
Ravi, M., Tentu, S., Baskar, G., Rohan Prasad, S., Raghavan, S., Jayaprakash, P., Jayaprakash, J., Rayala, S.K., Venkatraman, G., 2015. Molecular mechanism of anti-cancer activity of phycocyanin in triple-negative breast cancer cells. BMC Cancer 15(1), 768.
Roy, K.R, Arunasree, K.M., Reddy, N.P., Dheeraj, B., Reddy G.V., Reddanna, P., 2007. Alteration of Mitochondrial Membrane Potential by Arthrospira platensis C-phycocyanin Induces Apoptosis in the Doxorubicinresistant Human Hepatocellular-Carcinoma Cell Line HepG2, Biotechnology and Applied Biochemistry 47(-), 159-167.
Sarada, R., Pillai, M.G., Ravishankar, G.A., 1999. Phycocyanin from Arthrospira sp: influence of processing of biomass on phycocyanin yield, analysis of efficacy of extraction methods and stability studies on phycocyanin. Process Biochemistry 34(8), 795–801.
Serban, M.-C., Sahebkar, A., Dragan, S., Stoichescu-Hogea, G., Ursoniu, S., Andrica, F., Banach, M., 2015. A systematic review and meta-analysis of the impact of Spirulina supplementation on plasma lipid concentrations. Clinical Nutrition 35(4), 842-51.
Singh, N.K., Sonani, R.R., Prasad Rastogi, R., Madamwar, D., 2015. The phycobilisomes: an early requisite for efficient photosynthesis in cyanobacteria. Excli Journal 14(-), 268–289.
Soni, B., Kalavadia, B., Trivedi, U., Madamwar, D., 2006. Extraction, purification and characterization of phycocyanin from Oscillatoria quadripunctulata–isolated from the rocky shores of Bet-Dwarka, Gujarat, India. Process Biochemistry 41(-), 2017-2023.
Su, C.H., Liu, C.S., Yang, P.C., Syu, K.S., Chiuh, C.C., 2014. Solid-liquid extraction of phycocyanin from Arthrospira platensis: kinetic modeling of influential factors. Separation and Purification Technology 123(-), 64–68.
Sudhakar, M.P., Jagatheesan, A., Perumal, K., Arunkumar, K., 2015. Methods of phycobiliprotein extraction from Gracilaria crassa and its applications in food colorants. Algal Research 8 (-), 115–120.
Thengodkar, R.R., Sivakami, S., 2010. Degradation of Chlorpyrifos by an alkaline phosphatase from the cyanobacterium Arthrospira platensis. Biodegradation 21(-), 637-44.
Vernès, L., Granvillain, P., Chemat, F., Vian, M., 2015. Current Biotechnology 4(3), 1-11.
Walter, A., Carvalho, J.C.D., Soccol, V.T., Faria, A.B.B.D., Ghiggi, F., Soccol, C.R., 2011. Study of phycocyanin production from Arthrospira platensis under different light spectra. Brazilian Archives of Biology and Technology 54(-), 675-682.
Wu, H.L, Wang, G.H, Xiang, W.Z, Li,T., He, H., 2016. Stability and Antioxidant Activity of Food-grade Phycocyanin Isolated from Arthrospira platensis. International Journal of Food Properties 19(10), 2349-2362.
Yan, Y., Bao, Z., Shao, J., 2018. Phycocyanin concentration retrieval in inland waters: A comparative review of the remote sensing techniques and algorithms. Journal of Great Lakes Research 44(4), 748-755.

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