The effect of rosemary (Rosmarinus officinalis) hydroalcoholic extract on chemical, microbial and sensory indices of grouper fish fillet (Epinephelus coioides) during storage in refrigerator (40c)

Document Type : Research Paper


1 M.Sc. Student, Department of Fisheries, Faculty of Nano and Bio science and Technology, Persian Gulf University, Bushehr, Ira

2 Assistant Professor, Department of Fisheries, Faculty of Nano and Bio science and Technology, Persian Gulf University, Bushehr, Ira

3 Associate Professor, Department of Fisheries, Faculty of Nano and Bio science and Technology, Persian Gulf University, Bushehr, Ira

4 Assistant Professor, Department of Animal Sciences, Faculty of Agriculture and Natural Resources, Persian Gulf University, Bushehr, Iran



To investigate the effect of hydroalcoholic extract of rosemary (Rosmarinus officinalis) on chemical, microbial and sensory indices of grouper (Epinephelus coioides) in refrigerated conditions, four treatment groups: control treatment (uncoated fillets), fillet coated with rosemary 0.1%, Fillet covered with rosemary 0.2% and fillet covered with 0.4% rosemary were run. The 100 gr of samples were stored at refrigerator (4ºC) for 15 days. The measurements were undertaken at 0, 5, 10 and15 days at the beginning of the experiment. Generally, peroxide value (PV), thiobarbituric acid (TBA), total volatile base nitrogen (TVN), pH, counting total bacteria, counting cold-loving bacteria were significantly increased with storage time (P<0.05). The results showed that the use of rosemary extract improved the quality of chilled grouper.


Main Subjects

Aruoma, O., Halliwell, B., Aeschbach, R., Löligers, J., 1992. Antioxidant and pro-oxidant properties of active rosemary constituents: carnosol and carnosic acid. Xenobiotica 22(2), 257-268. DOI: 10.3109/00498259209046624
Basaga, H., Tekkaya, C., Acikel, F., 1997. Antioxidative and free radical scavenging properties of rosemary extract. LWT-Food Science and Technology 30(1), 105-108. DOI: 10.1006/fstl.1996.0127
Bubonja-Sonje, M., Giacometti, J., Abram, M., 2011. Antioxidant and antilisterial activity of olive oil, cocoa and rosemary extract polyphenols. Food Chemistry 127(4), 1821-1827. DOI: 10.1016/j.foodchem.2011.02.071
Dehghani, R., Tagavi motlagh, S. A., Kamrani, E., 1380. Estimation of growth parameters of common grouper in Hormozgan Journal of Marine Science and Technology 1(1), 17-25 (In Persian).
Dorman, H.D., Deans, S.G., 2000. Antimicrobial agents from plants: antibacterial activity of plant volatile oils. Journal of Applied Microbiology 88(2), 308-316.
Egan, H., Kirk, R., Sawyer, R., 1997. Pearson's composition and analysis of foods. 9th Editation Longman Scientific and Technical.
Ersoy, B., Aksan, E., Özeren, A., 2008. The effect of thawing methods on the quality of eels (Anguilla anguilla). Food chemistry, 111(2), 377-380. DOI: 10.1016/j.foodchem.2008.03.081
Fan, W., Chi, Y., Zhang, S., 2008. The use of a tea polyphenol dip to extend the shelf life of silver carp (Hypophthalmicthys molitrix) during storage in ice. Food Chemistry 108(1), 148-153. DOI: 10.1016/j.foodchem.2007.10.057.
Foods, I. A. o. M. S. I. C. o. M. S. F., Roberts, T., 1986. Microorganisms in Foods: Sampling for Microbiological Analysis, Principles and Specific Applications: Blackie Acad. & Professional.
Gao, W., Liu, Y.J., Tian, L.X., Mai, K.S., Liang, G.Y., Yang, H.J., Luo, W.J., 2010. Effect of dietary carbohydrate‐to‐lipid ratios on growth performance, body composition, nutrient utilization and hepatic enzymes activities of herbivorous grass carp (Ctenopharyngodon idella). Aquaculture Nutrition 16(3), 327-333. DOI: 10.1111/j.1365-2095.2009.00668.x
Ghanizade, M., Shapoori, M., Babazadeh, M., 2018. Effects of nano encapsulation of Rosmarinus officinalis on rainbow trout (Oncorhynchus mykiss) fillet shelf life in 4°c. Quarterly Journal of Experimental Animal Biology 6(4), 57-65. (In Persian)
Gogus, U., Smith, C., 2010. n‐3 Omega fatty acids: a review of current knowledge. International Journal of Food Science & Technology, 45(3), 417-436. DOI: 10.1111/j.1365-2621.2009.02151.x
Goulas, A.E., Kontominas, M.G., 2005. Effect of salting and smoking-method on the keeping quality of chub mackerel (Scomber japonicus): biochemical and sensory attributes. Food Chemistry 93(3), 511-520. DOI: 10.1016/j.foodchem.2004.09.040
Halliwell, B., & Chirico, S. 1993. Lipid peroxidation: its mechanism, measurement, and significance. The American Journal of Clinical Nutrition 57(5), 715S-725S. DOI: 10.1093/ajcn/57.5.715S
Hozbor, M., Saiz, A., Yeannes, M., Fritz, R., 2006. Microbiological changes and its correlation with quality indices during aerobic iced storage of sea salmon (Pseudopercis semifasciata). LWT-Food Science and Technology 39(2), 99-104. DOI: 10.1016/j.lwt.2004.12.008
Huss, H. H., Boerresen, T., Dalgaard, P., Gram, L., Jensen, B., 1998. Quality and quality changes in fresh fish. FAO, Documento Tecnico de Pesca (FAO).
Jafari, A., Jafarpour, A., Safari, R., 2017. Influence of chitosan nanocomposite and rosemary (Rosmarinus officinalis L.) extract coating on quality of Huso huso fillet inoculated with Listeria monocytogenes during refrigerated storage. Journal of Aquatic Food Product Technology 26(6), 675-685. DOI: 10.1080/10498850.2016.1266427
Karpińska, M., Borowski, J., Danowska-Oziewicz, M., 2001. The use of natural antioxidants in ready-to-serve food. Food Chemistry 72(1), 5-9. DOI: 10.1016/S0308-8146(00)00171
Khalafalla, F. A., Ali, F. H., & Hassan, A.-R. H. 2015. Quality improvement and shelf-life extension of refrigerated Nile tilapia (Oreochromis niloticus) fillets using natural herbs. Beni-Suef University Journal of Basic and Applied Sciences 4(1), 33-40. DOI: 10.1016/j.bjbas.2015.02.005
Kołakowska, A., Zienkowicz, L., Domiszewski, Z., Bienkiewicz, G., 2006. Lipid changes and sensory quality of whole-and gutted rainbow trout during storage in ice. Acta Ichthyologica et Piscatoria 36(1), 39-47. DOI: 10.3750/AIP2006.36.1.06
Kykkidou, S., Giatrakou, V., Papavergou, A., Kontominas, M., & Savvaidis, I. 2009. Effect of thyme essential oil and packaging treatments on fresh Mediterranean swordfish fillets during storage at 4 C. Food chemistry, 115(1), 169-175. DOI: 10.1016/j.foodchem.2008.11.083
Leung, A.Y., Foster, S., 1996. Encyclopedia of common natural ingredients used in food, drugs, and cosmetics: John Wiley & Sons, Inc.
Li, T., Hu, W., Li, J., Zhang, X., Zhu, J., Li, X., 2012. Coating effects of tea polyphenol and rosemary extract combined with chitosan on the storage quality of large yellow croaker (Pseudosciaena crocea). Food Control 25(1), 101-106. DOI: 10.1016/j.foodcont.2011.10.029
Lin, C.-C., Lin, C.-S., 2005. Enhancement of the storage quality of frozen bonito fillets by glazing with tea extracts. Food Control 16(2), 169-175. DOI: 10.1016/j.foodcont.2004.01.007
Massa, A. E., Palacios, D. L., Paredi, M. E., & Crupkin, M. 2005. Postmortem changes in quality indices of ice‐stored flounder (Paralichthys patagonicus). Journal of Food Biochemistry 29(5), 570-590. DOI: 10.1111/j.1745-4514.2005.00050.x
Mehdizadeh, T., Tajik, H., Jafarie, S., Kaboudari, A., 2019. Effect of Salvia officinalis L. extract on chemical, microbial, sensory and shelf life of rainbow trout fillet. Food Science and Biotechnology 28(5), 1499-1506. DOI: 10.1007/s10068-019-00575-y
Meshkibaf, M.H., Abdollahi, A., Ramandi, M.F., Sadati, S.A., Moravvej, A., Hatami, S., 2010. Antibacterial effects of hydro-alcoholic extracts of Ziziphora tenuior, Teucrium polium, Barberis corcorde and Stachys inflate. Koomesh 11(4), 240-245.
Millamena, O.M., 2002. Replacement of fish meal by animal by-product meals in a practical diet for grow-out culture of grouper Epinephelus coioides. Aquaculture, 204(1-2), 75-84. DOI: 10.1016/S0044-8486(01)00629-9
MOL, S., ERKAN, N., & VARLIK, C. 2002. The application of hypoxanthine activity as a quality indicator of cold stored fish burgers. Turkish Journal of Veterinary & Animal Sciences 26(2), 363-367.
Moslemi, M., Salehani, R., Mehdiabadi, S., 2019. Study of the Effect of Nisin and Rosemary Extract on Sensory and Microbial Spoilage Indicators of Rainbow Trout (Onchorynchus mykis) in Cold Storage Conditions. Journal of Animal Biology 11(4), 75-86. (In Persian)
Offord, E., Guillot, F., Aeschbach, R., Löliger, J., Pfeifer, A., 1997. Antioxidant and Biological Properties of Rosemary Components: Implications. Natural Antioxidants: Chemistry, Health Effects, and Applications 88.
Özyurt, G., Polat, A., Tokur, B., 2007. Chemical and sensory changes in frozen (− 18 C) wild sea bass (Dicentrarchus labrax) captured at different fishing seasons. International Journal of Food Science & Technology 42(7), 887-893. DOI: 10.1111/j.1365-2621.2006.01302.x
Pérez‐Mateos, M., Lanier, T.C., Boyd, L.C., 2006. Effects of rosemary and green tea extracts on frozen surimi gels fortified with omega‐3 fatty acids. Journal of the Science of Food and Agriculture 86(4), 558-567. DOI: 10.1002/jsfa.2388
Pons-Sánchez-Cascado, S., Vidal-Carou, M., Nunes, M., Veciana-Nogues, M., 2006. Sensory analysis to assess the freshness of Mediterranean anchovies (Engraulis encrasicholus) stored in ice. Food Control, 17(7), 564-569. DOI: 10.1016/j.foodcont.2005.02.016
Quitral, V., Donoso, M.L., Ortiz, J., Herrera, M.V., Araya, H., Aubourg, S.P., 2009. Chemical changes during the chilled storage of Chilean jack mackerel (Trachurus murphyi): Effect of a plant-extract icing system. LWT-Food Science and Technology 42(8), 1450-1454. DOI: 10.1016/j.lwt.2009.03.005
Raza, H., John, A., 2005. Green tea polyphenol epigallocatechin-3-gallate differentially modulates oxidative stress in PC12 cell compartments. Toxicology and Applied Pharmacology 207(3), 212-220. DOI: 10.1016/j.taap.2005.01.004
Reineccius, G., 1991. Off‐flavors in foods. Critical Reviews in Food Science & Nutrition 29(6), 381-402. DOI: 10.1080/10408399109527534
Ribeiro-Santos, R., Carvalho-Costa, D., Cavaleiro, C., Costa, H.S., Albuquerque, T.G., Castilho, M. C., Sanches-Silva, A., 2015. A novel insight on an ancient aromatic plant: The rosemary (Rosmarinus officinalis L.). Trends in Food Science & Technology 45(2), 355-368. DOI: 10.1016/j.tifs.2015.07.015
Richheimer, S. L., Bernart, M.W., King, G.A., Kent, M.C., Beiley, D.T., 1996. Antioxidant activity of lipid-soluble phenolic diterpenes from rosemary. Journal of the American Oil Chemists’ Society 73(4), 507-514. DOI: 10.1007/BF02523927
Rižnar, K., Čelan, Š., Knez, Ž., ŠKerget, M., Bauman, D., Glaser, R., 2006. Antioxidant and antimicrobial activity of rosemary extract in chicken frankfurters. Journal of Food Science 71(7), C425-C429. DOI: /10.1111/j.1750-3841.2006.00130.x
Rozman, T., Jersek, B., 2009. Antimicrobial activity of rosemary extracts (Rosmarinus officinalis L.) against different species of Listeria. Acta agriculturae Slovenica 93(1), 51.
Sallam, K. I., Ishioroshi, M., & Samejima, K. 2004. Antioxidant and antimicrobial effects of garlic in chicken sausage. LWT-Food Science and Technology 37(8), 849-855. DOI: 10.1016/j.lwt.2004.040001
Sarkardei, S., Howell, N.K., 2008. Effect of natural antioxidants on stored freeze‐dried food product formulated using horse mackerel (Trachurus trachurus). International Journal of Food Science &Technology 43(2), 309-315. DOI: 10.1111/j.1365-2621.2006.01435.x
Sengul, M., Yildiz, H., Gungor, N., Cetin, B., Eser, Z., Ercisli, S., 2009. Total phenolic content, antioxidant and antimicrobial activities of some medicinal plants. Pakistan Journal of Pharmaceutical Sciences 22(1), 102-106 .
Serdaroğlu, M., & Felekoğlu, E. 2005. Effects of using rosemary extract and onion juice on oxidative stability of sardine (Sardina pilchardus) mince. Journal of Food Quality 28(2), 109-120. DOI: 10.1111/j.1745-4557.2005.00016.x
Shahedi, F., Pegg, R.B., Saleemi, Z.O., 1995. Stabilization of meat lipids with ground spices. Journal of Food Lipids 2(3), 145-153. DOI: 10.1111/j.1745-4522.1995.tb00038.x
Standardization, E. O. f. 2005. Standard specifications for chilled and frozen fish fillets (3494) and (2–889). Egypt.
Tironi, V., Tomás, M., Añón, M., 2009. Lipid and protein changes in chilled sea salmon (Pseudopercis semifasciata): effect of previous rosemary extract (Rossmarinus officinalis L.) application. International journal of food science & Technology 44(6), 1254-1262. DOI: 10.1111/j.1365-2621.2009.01955.x
Wedgwood, H., & Atkinson, J. C. 1872. A dictionary of English etymology: Trübner & Company.
Yeh, S.-L., Kuo, C.-M., Ting, Y.-Y., Chang, C.-F., 2003. Androgens stimulate sex change in protogynous grouper, Epinephelus coioides: spawning performance in sex-changed males. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology 135(3), 375-382. DOI: 10.1016/S1532-0456(03)00136-4
Yingyuad, S., Ruamsin, S., Reekprkhon, D., Douglas, S., Pongamphai, S., Siripatrawan, U. 2006. Effect of chitosan coating and vacuum packaging on the quality of refrigerated grilled pork. Packaging technology and science: An international Journal 19(3), 149-157. DOI: 10.1002/pts.717