Biological control of cyanobacterial bloom in fish Culture ponds using Pseudomonas putida

Document Type : Research Paper


1 Ph. D graduate, Department of Fisheries, Faculty of Natural Resources, University of Tehran, Karaj, Iran

2 Associate Professor, Soil and Water Research Institute, Agricultural Research Education and Extension Organization, Karaj, Iran

3 Ph. D Student, Department of Aquaculture, Faculty of Marine Sciences, Tarbiat Modares University, Noor, Iran



Cyanobacterial blooms cause huge and irreparable financial losses to fish farmers every year. One of the most effective methods for managing water cyanobacterial bloom is biological control by adding pathogenic agents to break down cyanobacterial cells. Therefore, this study investigated the possibility of using Pseudomonas putida bacteria to control the bloom of cyanobacteria Chroococcus sp., Oscillatoria sp., Microcystis sp. and Gloeocapsa sp., isolated from fish Culture ponds was done in the laboratory. The results of this study showed that Pseudomonas putida bacteria have a significant effect on controlling all 4 cyanobacterial species. So, for the cyanobacterium Chroococcus sp. In the experimental group containing 106 CFU/ml of Pseudomonas putida bacteria, there was a moderate 80.7% reduction and also in the group containing 104 CFU/ml of this bacterium, there was 63.1% reduction compared to the control group models on the 10th day. Also, for the cyanobacterium Oscillatoria sp. at the 10th day, 78.9% reduction occurred in 104 CFU/ml and 87.7% in 106 CFU/ml. Next, for the cyanobacterium Microcystis sp. On the tenth day, there was 61.7 percent decrease in the group containing 104 CFU/ml and 70.5 percent decrease in the group containing 106 CFU/ml of Pseudomonas putida bacteria. Finally for the cyanobacterium Gloeocapsa sp. A decrease of 72.3% was observed in the experimental group containing 104 CFU/ml and an 83% decrease in the group containing 106 CFU/ml. Although the control group show an increase of 37-78 percent (P˂0.05). The results of this research showed that the bacterium Pseudomonas putida has a high efficiency in controlling the bloom of cyanobacteria.


Main Subjects

Anderson, D., 2009. Approaches to monitoring, control and management of harmful algal blooms (HABs). Ocean & Coastal Management 52(7), 342-347. DOI: 10.1016/j.ocecoaman.2009.04.006
Anderson, D.M., Burkholder, J.M., Cochlan, W.P., Glibert, P.M., Gobler, C.J., Heil, C.A., Kudela, R.M., Parsons, M. L., Rensel, J.E.J., Townsend, D.W., Trainer, V.L., Vargo, G.A., 2008. Harmful algal blooms and eutrophication: Examining linkages from selected coastal regions of the United States. Harmful Algae 8(1), 39-53. DOI: 10.1016/j.hal.2008.08.017
Armandeh, M., Mahmoodi, N., fallah, A.R., 2018. Isolation and identification of phosphate solubilizing bacteria from fish farms as a candidate for phosphorus biofertilizer. Journal of Aquatic Physiology and Biotechnology 6th year, 4th issue, pp. 121-140. (In Persian)
Boyd, C. E., 2002. Aquaculture pond bottom Soil quality management. Spine 11(6), 525-530.
Canelhas, M.R., 2011. The biocontrol potential of lytic bacteria against cyanobacterial blooms. Master thesis. project in biology science. Biology Education Centre and Limnology department, Uppsala University. Sweden. 41 p.
Geohab., 2001. Global Ecology and Oceanography of Harmful Algal Blooms Science Plan. SCOR and IOC press. Baltimore and Paris. 87 p.
Giordano, D., Coppola, D., Russo, R., Denaro, R., Giuliano, L., Lauro, F.M., Prisco, G., Verde, C., 2015. Marine Microbial Secondary Metabolites: Pathways, Evolution and Physiological Roles. Advances in Microbial Physiology 66, 357-428. DOI: 10.1016/bs.ampbs.2015.04.001
Johansson, C., Bergman, B., 2006. Reconstitution of the symbiosis of Gunnera manicata Linden: Cyanobacterial specificity. New Phytologist 126(4), 643-652. DOI: 10.1111/j.1469-8137.1994.tb02960.x
John, D.M., Museum, N.H., 2012. The Freshwater algal flora of the British Isles: an identification guide to freshwater and terrestrial algae. British Phycological Society Cambridge University Press. pp. 25-702.
Kim, M.J., Jeong, S.Y., Lee, S.J., 2008. Isolation, identification, and algicidal activity of marine bacteria against Cochlodinium polykrikoides. Journal of Applied Phycology 20(6): 1069-1078. DOI: 10.1007/s10811-008-9312-x
Komarek, J., Kastovsky, J., Mares, J., Johansen, J.R., 2014. Taxonomic classification of cyanoprokaryotes (Cyanobacterial genera) using a polyphasic approach. Preslia 86(4), 295-335.
Kristyanto, S., Kim, J., 2016. Isolation of marine algicidal bacteria from surface seawater and sediment samples associated with harmful algal blooms in Korea. Korean Journal of Microbiology 52(1), 40-48.
Kulik, M.M., 1995. The potential for using cyanobacteria (blue-green algae) and algae in the biological control of plant pathogenic bacteria and fungi. European Journal of Plant Pathology 101(6), 585-599. DOI: 10.1007/BF01874863
Padmavathi, P., Prasad-Durga, M.K., 2007. Egular Aper. Regular Paper 24, 32-43.
Pal, M., Yesankar, P. J., Dwivedi, A., Qureshi, A., 2020. Biotic control of harmful algal blooms (HABs): A brief review. Journal of Environmental Management 268 April, 110687.
Ren, H., Zhang, P., Liu, C., Xue, Y., Lian, B., 2010. The potential use of bacterium strain R219 for controlling of the bloom-forming cyanobacteria in freshwater lake. World Journal of Microbiology and Biotechnology 26(3), 465-472. DOI: 10.1007/s11274-009-0192-2
Roth, P.B., Twiner, M.J., Mikulski, C.M., Barnhorst, A.B., Doucette, G.J., 2008. Comparative analysis of two algicidal bacteria active against the red tide dinoflagellate Karenia brevis. Harmful Algae 7(5), 682-691. DOI: 10.1016/j.hal.2008.02.002
Safari, R., Yaghobzadeh, Z., 2020. Evaluation of the anti-algae activity of Pseudomonas isolates against the cyanobacterium Nodularia spumigena on a laboratory scale. Iran Scientific Journal of Fisheries 30, 187-193. (In Persian)
Sakine, S., Amir, H.M., Mahmood, A., Ali, R.M., Ramin, N., Reza, D., 2013. Performance evaluation of cyanobacteria removal from water reservoirs by biological method. African Journal of Microbiology Research 7(17), 1729-1734.
Su, J., Yang, X., Zhou, Y., Zheng, T., 2011. Marine bacteria antagonistic to the harmful algal bloom species Alexandrium tamarense (Dinophyceae). Biological Control, 56(2), 132–138.
Zhang, H., Yu, Z., Huang, Q., Xiao, X., Wang, X., Zhang, F., Wang, X., Liu, Y., Hu, C., 2011. Isolation, identification and characterization of phytoplankton-lytic bacterium CH-22 against Microcystis aeruginosa. Limnologica 41(1), 70-77. DOI: 10.1016/j.limno.2010.08.001
Zingone, A., Enevoldsen, H., 2000. The diversity of harmful algal blooms: a challenge for science and management. Ocean and Coastal Management 43(8), 725-748. DOI: 10.1016/S0964-5691(00)00056-9