The effect of oxytetracycline, neomycin sulfate and fluorophenicol antibiotics on growth and pigments contents of microalgae Chlorella vulgaris

Document Type : Research Paper

Authors

Department of Natural Resources, Isfahan University of Technology, Isfahan, Iran.

10.22059/jfisheries.2024.381183.1441

Abstract

The spread of antibiotics and their continued release into aquatic environments has adverse effects on non-target aquatic organisms such as phytoplankton. The aim of this study was to investigate the effect of three antibiotics, oxytetracycline, neomycin sulfate and fluorophenicol on the freshwater microalga Chlorella vulgaris in the laboratory conditions. In order to examin different concentrations of 0, 0.005, 0.05, 0.5, 5 and 50 mg/L of oxytetracycline, neomycin sulfate and fluorophenicol antibiotics on the size population, specific growth rate and pigments were studied. The results showed that there is a correlation between the type and concentration of antibiotics on different algae growth indices. Increasing the concentration of oxytetracycline led to an increase in cell density compared to the control treatment, but no significant difference was observed in specific growth rate and chlorophyll a, b and carotenoid levels (P>0.05). Low concentrations of the neomycin sulfate (0.005 and 0.05 mg /L) did not show a significant difference with the control treatment (P>0.05), while at higher concentrations (0.5, 5 and 50 mg/L) significant decrease in density and specific growth rates was observed compared to the control (P<0.05). In fluorophenicol, except for the 0.05 mg/L treatment, which resulted in a slight increase in cell density, specific growth and chlorophyll a value compared to the control treatment, there was no significant difference between the other treatments (P>0/05). This study showed that the response of C. vulgaris to the examined antibiotics is rapid, inexpensive and easily measurable; therefore, C. vulgaris can be used for antibiotics effects in aquatic environments.

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