Document Type : Research Paper
Authors
1
iranian fisheries science research institute
2
1. Assistant Professor, Persian Gulf and Oman Sea Ecological Cener, Iranian Fisheries Science Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Bandar Abbas, Iran.
3
Caspian Institute of Ecology
Abstract
Sea cucumber is one of the most important species of Echinodermata, belongs to the category of Holothuroidea. Their well-known bioactive compounds have anti-cancer, anticoagulant, antihypertensive, anti-inflammatory, antimicrobial, antioxidant, anti-arterial, and wound-healing effects. In this study, the anti-inflammatory effect of methanolic extract on the muscular body wall of sea cucumbers Holothuria leucospilota on Albino rats was investigated. Sea cucumbers were collectted from the Hengam island, dried and extracted with hexane, ethyl acetate and methanol. The extracts were evaluated by lipoxygenase enzymes inhibition test in in vitro conditions. In in vivo experiments, the animals were divided into 5 groups of 6 rats, including: control, treated with carrageenan, treated with MEHL extract (100/200 mg / kg) and aspirin 10 mg/kg. In order to evaluate the inflammation, carrageenan test was used in Hind paw edema method. In in vitro experiments, methanolic extract (MEHL) with 43.75± 2.83 and ethyl acetate and hexane extract showed 12.92 ± 1.22 and 9.74 1.57 percent inhibition of lipoxygenase enzymes, respectively. Methanolic extract (MEHL) Holothuria leucospilota was selected for further in vivo testing. In animal experiments, MEHL, with concentrations of 100 and 200 mg/kg, inhibited hind paw edema at 3 and 4 hours, with 13.62 ± 1.3 and 35.15 ± 2.5 and , 18.41 ± 1.7 and 45.22 ± 3.2 percent, respectively. Holothuria leucospilota methanolic extract contains anti-inflammatory compounds that have shown better anti-inflammatory effects compared to the anti-inflammatory drug acetylsalicylic acid.
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