امکان تعیین جنسیت قزل آلای رنگین کمان (Oncorhynchus mykiss) با استفاده از نشانگرهای ملکولی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشیار دانشکدة منابع طبیعی، دانشگاه تهران، کرج، ایران

2 دانش‌آموختۀ کارشناسی ارشد مهندسی شیلات، دانشکدة منابع طبیعی، دانشگاه تهران، کرج، ایران

چکیده

 





چکیده






این تحقیق با هدف بررسی امکان تعیین جنسیت ماهی قزل‌آلای رنگین‌کمان از طریق غربالگری مولکولی به منظور ایجاد جمعیت‌های تک‌جنسیت انجام شد. بدین منظور تعداد 35 نمونه از بافت بالة دمی ماهیان بالغ قزل‌آلای رنگین‌کمان بازار کرج، به‌منزلة نمونة آماری، تهیه شد و پس از استخراج DNA از نمونه‌ها، تکثیر جایگاه نشانگرها انجام پذیرفت. واکنش زنجیره‌ای پلیمراز برای تکثیر نشانگرهای وابسته به جنسیت در ماهیان قزل‌آلای رنگین‌کمان بر اساس دو نشانگر OmyFA و OmyFATU تنظیم شد. در نهایت جمعیت مذکور بر اساس حضور یا نبود باندی مخصوص به جنسیت برای نشانگرها غربالگری شد. نتایج این پژوهش حضور نشانگرهای OmyFA و OmyFATU در یک جنسیت (نر) را با دقت 100 و 55 درصد گزارش کردند. بر اساس این نتایج نشانگر OmyFA برای غربال نمونة آماری مذکور (قزل‌آلای رنگین‌کمان بازار کرج) پیشنهاد می‌شود.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Investigate the possibility of Sex determination of rainbow trout (Oncorhynchus mykiss) using molecular markers

نویسندگان [English]

  • Hamid Farahmand 1
  • Mohammad Akhavan Bahabadi 2
  • Mohammad ali Nematollahi 1
  • Aliraza Mirvaghefi 1
1 Associate Professor, Department of Fisheries, Faculty of Natural Resources, University of Tehran, Tehran, Iran
2 MSC in Fisheries Engineering, Department of Fisheries, Faculty of Natural Resources, University of Tehran, Tehran, Iran
چکیده [English]

The study investigates the possibility of sex determination of rainbow trout (Oncorhynchus mykiss)
using molecular screen for products monosex of populations. 35 caudal fin tissue samples were
obtained from Karaj Rainbow trout mature population and then genomic DNA was extracted and
marker loci amplified. Polymerase chain reaction (PCR) was setup based on two omyFA and
OmyFATU markers for amplification of sex linked markers in rainbow trout. Finally, the population
was screened based on presence of a specific band for both markers. Result of this expriments showed
that presence of omyFA and OmyFATU markers in males were %100 and %55 respectively. Acording
to the result omyFATU is proper marker for screen of karaj trout population.

کلیدواژه‌ها [English]

  • monosex culture
  • Rainbow trout
  • sex-associated markers
  • sex determination
  • sex differentiation

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