بررسی تنوع ژنتیکی ماهی کپور معمولی (Cyprinus carpio) در آب های ایرانی دریای خزر با استفاده از نشانگرهای ریزماهواره ای

نوع مقاله: مقاله پژوهشی

نویسندگان

1 کارشناس ارشد، بخش بیوتکنولوژی، پژوهشکدة اکولوژی دریای خزر، ساری، ایران

2 استاد مؤسسة تحقیقات شیلات ایران، تهران، ایران

3 کارشناس، بخش بیوتکنولوژی، پژوهشکدة اکولوژی دریای خزر، ساری، ایران

چکیده

تنوع ژنتیکی جمعیت‌‌های ماهی کپور معمولی (Cyprinus carpio) در حوزة جنوبی دریای خزر با نمونه‌برداری 120 قطعه ماهی کپور معمولی از سواحل استان گلستان، مازندران و گیلان با استفاده از ده جفت پرایمر ریزماهواره بررسی شد. نتایج نشان داد که هشت جایگاه ریزماهواره‌ای از ده جایگاه مورد بررسی، چندشکل بودند. میانگین تعداد آلل‌های مشاهده‌شده و مؤثر به ترتیب 08/7 و 29/4 بود و حداکثر ناخالصی مشاهده‌شده و مورد انتظار به ترتیب 95/0 و 92/0 محاسبه شد. در بررسی تعادل هاردی- واینبرگ مناطق گلستان، گیلان و مازندران در تمامی جایگا‌ه‌های مورد بررسی، به استثنای جایگاه Syp8، خارج از تعادل هاردی- واینبرگ بود (05/0P<). بر پایة محاسبات انجام‌شده حداکثر FST (028/0) بین نمونه‌های گلستان و مازندران بود که دارای کمترین میزان جریان ژنی (4/1) است و حداقل FST (004/0) بین نمونه‌های مازندران و گیلان با بیشترین میزان جریان ژنی (43/3) مشاهده شد. بیشترین فاصلة ژنتیکی بین نمونه‌های منطقة گلستان و مازندران 084/0 و کمترین آن 033/0 بین نمونه‌های منطقة مازندران و گیلان بوده است. با توجه به نتایج آزمون AMOVA و محاسبة FST، همچنین تفاوت ژنتیکی معنا‌دار بین نمونه‌ها (01/0P<)، می‌توان بیان کرد که جمعیت واحدی از کپور معمولی در مناطق مورد بررسی وجود ندارد و حداقل سه گروه ژنتیکی متفاوت از این گونه یافت می‌شود.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Investigation of common carp (Cyprinus carpio) genetic diversity in Iranian waters of the Caspian Sea by microsatellite markers

نویسندگان [English]

  • Faramarz Laloei 1
  • Sohrab Rezvani Gilkolaei 2
  • Mohammad-Javad Taghavi 3
1 M.Sc., Biotechnology Department, Ecology Research Center of Caspian Sea, Sari, Iran
2 Professor, Iranian Fisheries Research Organization, Tehran, Iran
3 Expert, Biotechnology Department, Ecology Research Center of Caspian Sea, Sari, Iran
چکیده [English]

Population genetic structure of common carp (Cyprinus carpio) were examined by 120 samples from
Gilan, Mazandaran and Golestan coastal using 10 loci microsatellite. Eight of ten microsatellite loci
were polymorphism. The mean of observed and effective allele number was 7.08 and 4.29 respectively
and also the maximum of observed and expected heterozygosity was 0.95 and 0.92 respectively. It was
seen that specimens from all regions and loci (exception locus Syp8) were not in Hardy-Weinberg
Equibrium in all of the loci (P<0.05). Based on Analysis of Molecular Variance (AMOVA), the
maximum FST (0.028) and minimum Nm (3.7) was between Golestan and Mazandaran. Also, the
minimum FST and maximum Nm was between Gilan and Mazandaran. Highest genetic distance was
between Golestan and Mazandaran (0.084) and lowest genetic distance was 0.33. As result, there is a
significant genetic divergence between some of samples (P<0.01). Therefore, there are at least three
genetic group of Cyprinus carpio in Southern part of the Caspian Sea.

کلیدواژه‌ها [English]

  • Caspian Sea
  • Cyprinus carpio
  • Genetic diversity
  • Iran
  • Microsatellite

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