بررسی تأثیر ویتامین C جیرة غذایی در رشد، ترکیب شیمیایی بدن و فعالیت برخی آنزیم‌های آنتی‌اکسیدانی بچه‌ماهی نورس آزاد دریای خزر (Salmo trutta caspius

نوع مقاله : مقاله پژوهشی

نویسندگان

1 کارشناس ارشد تکثیر و پرورش آبزیان دانشکدة علوم دریایی دانشگاه تربیت مدرس

2 استاد گروه تکثیر و پرورش آبزیان دانشکدة علوم دریایی دانشگاه تربیت مدرس

3 استادیار گروه شیلات دانشکده منابع طبیعی و محیط زیست ،دانشگاه ملایر

چکیده

به‌ منظور بررسی عملکرد ویتامین C در ترکیب شیمیایی بدن و فعالیت آنزیم‌های آنتی‌اکسیدانی شامل سوپراکسید دیسموتاز و کاتالاز در بچه‌ماهی نورس آزاد دریای خزر (Salmo trutta caspius)، پنج جیرة غذایی حاوی سطوح مختلف ویتامین C (صفر (شاهد)، 50، 250، 750 و 1500 میلی‌گرم ویتامین به ‌ازای هر کیلوگرم غذا) در سه تکرار و به‌ مدت شش هفته به بچه‌ماهیان با میانگین وزنی 02/0± 1/0 گرم خورانده شد. پس از گذشت شش هفته پرورش، نتایج شاخص‌های رشد و زنده‌مانی اختلاف معنی‌داری در بین تیمارها نشان داد (05/0>P) پایین‌ترین درصد میانگین افزایش وزن (BWG)، نرخ رشد ویژه (SGR) و زنده‌مانی در گروه شاهد و بالاترین درصد این فاکتورها در گروه تغذیه‌شده با سطح mg/kg 750 مشاهده شد. میزان تجمع ویتامین C در بافت با افزایش آن در جیرة غذایی رابطة مثبتی نشان داد. نیاز ویتامین C بر اساس درصد افزایش وزن بدن mg/kg 1/569 تخمین زده شد. نتایج بررسی ترکیب شیمیایی بدن نشان داد که مقادیر رطوبت، خاکستر، چربی و پروتئین اختلاف معنی‌داری بین تیمارها ندارند (05/0 <P). تأثیر تست استرس محدودیت اکسیژنی در فعالیت آنزیم‌های آنتی‌اکسیدانی سوپراکسید دیسموتاز و کاتالاز نشان داد که فعالیت این آنزیم‌ها بعد از اعمال تست استرس نسبت به قبل از آن افزایش معنی‌داری در تیمار کنترل داشته است (05/0>P) در حالی که فعالیت آنزیمی سایر تیمارها پس از اعمال استرس اختلاف معنی‌داری نسبت به قبل از استرس نشان نداد (05/0<P). بر اساس نتایج، سطح mg/kg 750 ویتامین C برای رشد و زنده‌مانی بچه‌ماهیان نورس آزاد دریای خزر پیشنهاد می‌شود.
 

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Assessment of vitamin C effect on body composition and some antioxidant enzyme activities in Caspian brown trout fry (Salmo trutta caspius)

نویسندگان [English]

  • Maryam Khajavi 1
  • Abdolmohammad Abedian Kenari 2
  • Abas Zamani 3
1 The graduated M.Sc in Fisheries from Marine Sciences Faculty of Tarbiat Modares University, Noor, Iran
2 The Professor in Fisheries Department of Marine Sciences Faculty of Tarbiat Modares University,Noor, Iran
چکیده [English]

   In order to study of vitamin C effect on body composition and antioxidant enzyme activities, including superoxide dismutase and catalase in five diets containing different levels of vitamin C (0, 50, 250, 750and 1500mg vitamin/kg diet) had been fed by Caspian brown trout fry (Salmo trutta caspius) for 6 weeks in triplicate. After 6 weeks, the findings of growth parameters and survival showed significant difference among groups (P< 0.05). The lowest amount of SGR, BWG and survival was observed in control group while the group of 750 mg/kg had the highest amount of  SGR, BWG and survival than those from others. The vitamin C concentration in tissue correlated positively with increasing its levels in diets. The vitamin C requirements were estimated to be 569.1 mg kg-1 based on body weight gain. Body composition including moisture, ash, fat and protein showed no significant difference among groups (P>0/05). The effect of confinement stress on antioxidant enzymes activity of control diet was indicated a sigtnificat increase in activity after stress than that from before sttress (P < 0.05), While other diets showed no significatnt diference between pre-stress and after stress (P > 0.05). The findings suggest that the 750 mg/kg vitamin C could be affected on growth and survival in caspian brown trout fty.
 
 
 

کلیدواژه‌ها [English]

  • vitamin c
  • Catalase
  • superoxide dismutase
  • Caspian brown trout fry
  • Body Composition

مراجع

 
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