بررسی اثر درجۀ حرارت در غلظت کشندة (LC50 96-h) سم سایپرمترین (Cypermethrin) در ماهی سفید دریای خزر (Rutilus frisii kutum, Kamensky, 1901)

نوع مقاله: مقاله پژوهشی

نویسندگان

1 کارشناس ارشد، گروه شیلات، دانشکدة منابع طبیعی دانشگاه تهران

2 دانشیار گروه شیلات، دانشکدة منابع طبیعی دانشگاه تهران

چکیده

طی این تحقیق سمیت حاد[1] حشره‌کش سایپرمترین در سه سطح دمایی مختلف (15 و  20 و 25 درجۀ سانتی‌گراد) در بچه‌ماهیان 1±3 گرمی (میانگین± SD) سفیدماهی دریای خزر (Rutilus frisii kutum Kamensky, 1901) به منظور تعیین غلظت کشندة 50 درصد از جمعیت ماهیان در 96 ساعت مطالعه و بررسی شد. آزمایش‌ها به صورت ساکن[2] و بر اساس روش استانداردO.E.C.D  به مدت 4 شبانه روز (96ساعت) انجام و پارامترهای مؤثر فیزیکوشیمیایی آب از جمله pH، سختی کل، اکسیژن محلول و درجۀ حرارت کنترل شد. آزمایش‌های ابتدایی به منظور یافتن محدودة کشندگی با رهاسازی بچه‌ماهیان به داخل آکواریوم‌هایی که به حجم 20 لیتر آبگیری و به طور مداوم هوادهی می‌شدند به تعداد 10 عدد بچه‌ماهی برای هر آکواریوم انجام شد و پس از به‌دست‌آمدن محدودة کشندگی تیمارهای نهایی با 6 تیمار و 3 تکرار مشخص شد و در نهایت بر اساس نتایج و با استفاده از آنالیز پروبیت، مقادیرLC10 ، LC30، LC50، LC70، LC90 و LC99 در 24، 48، 72 و 96 ساعت برای هر سه سطح دمایی بر روی سفیدماهی اندازه‌گیری شد و سمیت حاد (LC50 96-h) سم سایپرمترین برای بچه‌ماهی سفید در دماهای ºC 1±15،  ºC1±20 و ºC 1±25 به ترتیب برابر54/1، 49/1 و 30/1 میکروگرم در لیتر به دست آمد. نتایج نشان داد با افزایش درجۀ حرارت سمیت سایپرمترین افزایش می‌یابد و مرگ‌ومیر بیش‌تری برای ماهیان اتفاق می‌افتد. در این آزمایش از نظر بالینی علائمی مانند خون‌مردگی در ناحیة ساقة دمی، تنفس غیرعادی، شنای نامنظم در ماهیان مورد آزمایش مشاهده شد.
 

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Effect of temperature lethal concentration (LC50 96-h) of Cypermethrin on the Caspian kutum fish Rutilus frisii Kutum (Kamensky, 1901)

نویسندگان [English]

  • Saeid Shahbazi 1
  • Alireza Mirvaghefi 2
  • Majid Abedi 1
  • Mehdi Taherian 1
1 MSc. Department of Fisheries, Faculty of Natural Resources, University of Tehran, Iran.
2 Associate prof. Department of Fisheries, Faculty of Natural Resources, University of Tehran, Iran.
چکیده [English]

In this study, the acute toxicity of Cypermethrin insecticides on caspian kutum fry (Rutilus frisii Kutum Kamensky, 1901) with the weight of 3 ± 1 g (average ± SD) in three different temperatures (15 °C , 20 °C and  25 °C) measured. The aim of this study was to determine the lethal concentration of 50 % of fish population within 96 hours. According to O.E.C.D standard methods, Experiments conditions were static and lasted for 4 days (96 h). Water physico-chemical parameters such as pH, total hardness, dissolved oxygen and temperature mesearued During the experiment. To find out cypermethrin cytotoxic range, 10 sea kutum fry were placed in each 20 Litre aquarium and aerated during the experiment. After obtaining leathal concentration final experiment started with 6 treatment and 3 repeatation. The values of LC10, LC30, LC50, LC70, LC90 and LC99 at 24, 48, 72 and 96 hours for all three temperature treatments obtained By using probit analysis, and Cypermethrin acute toxicity (LC50 96-h) for caspian kutum fry at 15 ± 1 ºC, 20 ± 1 ºC and 25 ± 1 ºC was 1.54, 1.49 and 1.30 micro grame per Litre, respectively. Results showed that by increasing the temperature Cypermethrin toxicity increases and also more deaths occur. In this experiment, the Clinical signs such as caudal region bruises, abnormal breathing and fish irregular swimming observed.
 

کلیدواژه‌ها [English]

  • Insecticide
  • Cypermethrin
  • Caspian Kutum (fish Rutilus frisii)
  • temperature
  • LC50 96-h
 

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