بررسی غلظتهای مختلف یونهای سدیم و پتاسیم بر شدت فعالیت آنزیم Na/K-ATPase و شاخص‌های کیفی لارو در مراحل مختلف لاروی میگوی بزرگ آب شیرین (Macrobrachium rosenbergii)

نوع مقاله : مقاله پژوهشی

نویسندگان

1 1. استادیار گروه مهندسی شیلات دانشکدة منابع طبیعی دانشگاه تهران

2 2. استاد گروه مهندسی شیلات دانشکدة منابع طبیعی دانشگاه تهران

چکیده

در مراحل اولیة لاروی میگوی بزرگ آب شیرین دستگاه تنظیم اسمزی هنوز شکل نگرفته است و تنظیم اسمزی و یونی بدن عمدتاً مربوط به فعالیت‌های آنزیم Na/K-ATPase با مصرف ATP می باشد. به همین دلیل در چرخة زیست طبیعی، مراحل لاروی باید در آبهای لب‌شور نزدیک مناطق مصبی با شرایط مشابه اسمزی ایزوتونیک بدن لارو با حداقل مصرف ATP و حداکثر بازماندگی سپری شود. این تحقیق با هدف بررسی تأثیر غلظت‌های مختلف سدیم و پتاسیم در آب مرکز تکثیر، بر شدت فعالیت آنزیم Na/K-ATPase، شاخص کیفی لارو (LCI) و بازماندگی در مراحل 1، 4، 7 و 11 لاروی میگوی بزرگ آب شیرین انجام گردید. برای این تحقیق، از ترکیب پایة آب لب‌شور مصنوعی نیو (شوری 12 گرم در لیتر) استفاده شد و دو آزمایش در مخازن 100 لیتری با تراکم اولیة 200 لارو در لیتر انجام گردید. در آزمایش اول، چهار تیمار 100، 150، 200 و 250 میلی‌گرم در لیتر پتاسیم با استفاده از نمک کلرید پتاسیم و در آزمایش دوم، چهار تیمار 2000، 3000، 4000 و 5000 میلی‌گرم در لیتر سدیم با استفاده از نمک کلرید سدیم هر کدام با سه تکرار فراهم شد. بر اساس نتایج به دست آمده، کیفیت لارو میگوی بزرگ آب شیرین بر اساس شدت فعالیت آنزیم Na/K-ATPase شاخص بهتری نسبت به شاخص کیفی لارو و بازماندگی لاروها می‌باشد. نتایج نشان داد که شرایط ایزوتونیک و حداقل فعالیت آنزیم Na/K-ATPase در مراحل 1 و 4 لاروی و مراحل 7 و  11 لاروی به ترتیب 4000 و 3000 میلی‌گرم در لیتر سدیم و 150 میلی‌گرم درلیتر پتاسیم برای هر چهار مرحلة لاروی می‌باشد.  

کلیدواژه‌ها

عنوان مقاله [English]

Effects of Various Sodium and Potassium Concentrations Mediums on Na/K-Atpase Activity and Condition Indices at Different Larval Stages of Macrobrachium rosenbergii

نویسندگان [English]

  • Kamran Rezaei Tavabe1* 1
  • Gholamreza Rafiee2 2
1 1. Assistant Professor, Fisheries Department, Natural Resources Faculty, University of Tehran
2 2. Professor, Fisheries Department, Natural Resources Faculty, University of Tehran
چکیده [English]

Macrobrachium rosenbergii lifecycle is unique so that the adults thrive in freshwater and larval development requires brackish water environments near to estuaries as close as possible to larval body isosmotic for minimum Na/K-ATPase activity and maximum ATP storage. Osmoregulation system at early larval stages is not formed and larval osmoregulation is mainly attributed to Na/K-ATPase activity. The present study aimed to evaluate effects of various sodium and potassium concentrations on Na/K-ATPase activity, larval condition index (LCI) and survival rate of M. rosenbergii at 1st, 4th, 7th and 11th larval stages. Triplicate treatments of Sodium (2000, 3000, 4000 and 5000 ppm) and potassium (100, 150, 200 and 250 ppm) were prepared separately in tanks (100‑L) and initial larvae density was fixed 200 larvae L-1. The treatments were set up at 12 ppt salinity based on New artificial brackish water composition. To adjust desired concentrations of sodium and potassium for the treatments, their soluble salts, including NaCl and KCl, were used. The results indicated that evaluation of the freshwater prawn larval quality according to Na/K-ATPase activity is a better index than LCI and survival rate. The results also showed that isotonic condition and minimum Na/K-ATPase activity at 1st and 4th larval stages and 7th and 11th stages occurred respectively in 4000 and 3000 ppm sodium treatments but this index for potassium treatments was 150 ppm for the all larval stages.

کلیدواژه‌ها [English]

  • Freshwater Prawn
  • Na/K-ATPase Activity
  • Larval Condition Index
  • Osmoregulation
  • Isotonic

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شیلات
دوره 69، شماره 2
شهریور 1395
صفحه 145-154

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