تنوع و ساختار ژنتیکی جمعیت‌های سیاه ماهی خالدار Capoeta trutta (Heckel, 1843) در استان کردستان با استفاده از نشانگرهای بین ریز ماهواره‌ای

نوع مقاله: مقاله پژوهشی

نویسندگان

1 1. دانش‌آموخته کارشناسی ارشد، گروه شیلات دانشکده منابع طبیعی دانشگاه زابل

2 2. دانشیار گروه شیلات دانشکده منابع طبیعی دانشگاه زابل

3 3. استادیار گروه شیلات دانشکده منابع طبیعی دانشگاه کردستان

4 4. استادیار گروه شیلات دانشکده منابع طبیعی دانشگاه زابل

چکیده

به منظور بررسی میزان تنوع ژنتیکی و تعیین ساختار ژنتیکی جمعیت‌های سیاه ماهی خالدار (Capoeta trutta) در دو حوزه آبریز واقع در استان کردستان از نشانگرهای بین ریزماهواره‌ای استفاده گردید. از شش آغازگر دی‌نوکلئوتیدی مورد استفاده، تنها در سه آغازگر (AG)9C، (GA)9C و (AC)9T باندهای واضح، تکرارپذیر و قابل امتیازدهی در شرایط واکنش PCR مورد استفاده مشاهده گردید. این سه آغازگر در مجموع تعداد 56 باند تکرار پذیر در شش جمعیت مورد مطالعه تولید نمودند که درصد پلی‌مورفیسم در جمعیت رودخانه‌های سیروان، گاوه رود، قشلاق، از حوزه سیروان و رودخانه‌های چومان، گرماب و شوی از حوزه چومان به ترتیب 00/80، 98/82، 61/82، 71/85، 60/79 و 49/80 تعیین گردیدند. میزان هتروزیگوسیتی در جمعیت‌های مورد بررسی متوسط تا زیاد برآورد گردید. شاخص‌های تنوع ژنتیکی درون جمعیتی در جمعیت چومان به طور معنی‌دار بیشتر از سایر جمعیت‌های مورد مطالعه بود. نتایج تجزیه واریانس مولکولی نشان داد که حدود 89 درصد تنوع ژنتیکی مربوط به درون جمعیت‌ها بوده و تنها 11 درصد آن مربوط به تنوع بین جمعیتی می‌باشد. مقدار شاخص تثبیت (Fst) محاسباتی نشان‌دهنده میزان تمایز کم تا متوسط در شش جمعیت مورد بررسی است. با این وجود نتایج آزمون صحت، تمایز معنی‌داری بین جمعیت‌های مورد مطالعه نشان نداد. نتایج این تحقیق نشان می‌دهد که شرایط زیست و حفظ تنوع ژنتیکی برای گونه سیاه ماهی خالدار در دو زیستگاه‌ سیروان و چومان در حد متوسط می‌باشد.  

کلیدواژه‌ها


عنوان مقاله [English]

Diversity and Genetic Structure of Capoeta trutta (Heckel, 1843) Populations in the Kurdistan Province using Inter Simple Sequence Repeat Markers

نویسندگان [English]

  • Behroz Mirzaei1 1
  • Ebrahim Alizadeh Doughikollaee2 2
  • Barzan Bahrami Kamangar3* 3
  • Ali Arshadi4 4
1 1. Msc of Fisheries, Department of Fisheries, Faculty of Natural Resources, University of Zabol, Zabol, Iran
2 2. Associate Professor, Department of Fisheries, Faculty of Natural Resources, University of Zabol, Zabol, Iran
3 3. Assistant Professor, Department of fisheries, Faculty of Natural Resources, University of Kurdistan, Sanandaj, Iran
4 4. Assistant Professor, Department of Fisheries, Faculty of Natural Resources, University of Zabol, Zabol, Iran
چکیده [English]

Inter Simple Sequence Repeat (ISSR) analysis was performed to evaluate genetic diversity and genetic structure of six Capoeta trutta populations from two main watersheds in the Kurdistan province. Fish samples were caught from the Choman, Shooei, Garmab Rivers in the Choman watershed and from the Gheshlagh, Gavarood, Sirvan Rivers in the Sirvan watershed. From six used dinucleotide primers, three ((AG)9C, (GA)9C and (AC)9T)) generated discernible bands. Fifty six clear and repeatable bands were generated throughout six populations. The percentages of polymorphism were 80.00, 82.98, 82.61, 85.71, 79.60 and 80.49 within the Sirvan, Gavarood, Gheshlagh, Choman, Garmab and Shooei populations, respectively. The average values of heterozygosites were determined as moderate to high within the studied populations. The intrapopulation genetic diversity indices were significantly higher in the Choman population than others.  The analysis of molecular variance revealed 89 percent variation within populations whereas only 11 percent between populations. Population comparisons based on fixation index (Fst) revealed low to moderate differentiation between six studied populations. However there was no significant differentiation between populations as testified by exact test. The results showed that environmental conditions to maintain genetic diversity of Capoeta trutta populations in two watersheds are moderate.

کلیدواژه‌ها [English]

  • Genetic diversity
  • genetic structure
  • ISSR Markers
  • Capoeta trutta
  • Kurdistan
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