اثرات گرسنگی و غذادهی مجدد بر بازیابی ظرفیت آنزیم‌های گوارشی ماهی صبیتی Sparidentex hasta Sparidentex hasta

نوع مقاله : مقاله پژوهشی

نویسندگان

1 فارغ التحصیل دانشگاه علوم و فنون دریایی خرمشهر

2 استاد گروه شیلات، دانشکده منابع طبیعی دریا، دانشگاه علوم و فنون دریایی خرمشهر، ایران

3 استاد گروه شیلات، دانشکده منابع طبیعی دریا، دانشگاه علوم و فنون دریایی خرمشهر.

4 موسسه تحقیقاتی واکسن و سرم سازی رازی شعبه اهواز

10.22059/jfisheries.2022.344371.1334

چکیده

در این تحقیق توانایی ماهی صبیتی در تنظیم و بازیابی ظرفیت آنزیم‌های گوارشی (تریپسین، کیموتریپسین و لیپاز) طی دوره‌های مختلف محرومیت غذایی و غذادهی مجدد به مدت 80 روز مورد مطالعه قرار گرفت. 300 ماهی با میانگین وزن اولیه 24/0±47/28 گرم در دوازده مخزن پرورشی 300 لیتری از جنس پلی اتیلن ذخیره سازی شدند. چهار تیمار آزمایشی شامل تیمار 1- ماهیان دو روز گرسنگی و هشت روز تغذیه، در تیمار 2 -ماهیان چهار روز گرسنگی و شانزده روز تغذیه و تیمار 3 -ماهیان هشت روز گرسنگی و سی و دو روز تغذیه، بودند. این دوره‌های گرسنگی و تغذیه،  تا پایان آزمایش (80 روز) برای تیمار اول هشت بار، برای تیمار دوم چهار بار و برای تیمار سوم دو بار تکرار شد. در تیمار شاهد ماهیان در تمام مدت آزمایش تا حد سیری تغذیه شد. در پایان دوره محرومیت غذایی سطح فعالیت آنزیم تریپسین و کیموتریپسین در گروه شاهد به طور معنی داری بالاتر از سطح فعالیت سایر تیمارها بود (05/0P<). سطح فعالیت آنزیم لیپاز در چرخه اول تیمار 1 و 2 اختلاف معنی داری با گروه شاهد نداشت. هر چند که فعالیت این آنزیم در چرخه میانی تیمار 2 به طور معنی‌داری کمتر از گروه شاهد بود (05/0P<). در پایان روزهای 40 و 80، هیچ گونه اختلاف معنی‌داری بین فعالیت سه آنزیم گوارشی بین گروه شاهد و تیمارها مشاهده نشد. نتایج تحقیق حاضر نشان داد که ماهیان Sparidentex hasta توانایی بازیابی ظرفیت گوارشی خود پس از رفع دوره‌های مختلف محرومیت غذایی هستند.

کلیدواژه‌ها

عنوان مقاله [English]

Effects of starvation and re-feeding on recovery of digestive enzyme capacity in Sobeity (Sparidentex hasta) marine fish

نویسندگان [English]

  • Nima Yazdi 1
  • Mohammad Zakeri 2
  • Preeta Kochanian 3
  • Seyed Mohammad Mousavi 3
  • Ahmad Taghavi Moghadam 4
1 M.Sc. Graduate, Department of Fisheries, Faculty of Marine Natural Resources, Khorramshahr University of Marine Science and Technology, Khorramshahr, Iran.
2 Professor, Department of Fisheries, Faculty of Marine Natural Resources, Khorramshahr University of Marine Science and Technology, Iran.
3 Professor, Department of Fisheries, Faculty of Marine Natural Resources, Khorramshahr University of Marine Science and Technology, Iran.
4 Razi Vaccine and Serum Research Institute, Ahvaz, Iran
چکیده [English]

In this study, the ability of the Sobeity fish to regulate and restore the capacity of digestive enzymes (trypsin, chymotrypsin, and lipase) during different periods of starvation and re-feeding was studied for 80- day period. 300 fish with an average initial weight of 28.47±0.24 g was stocked in twelve 300-liter polyethylene tanks. In four experimental treatments, including treatment 1 (T1), the fish were starved for two days and fed for eight days; in treatment 2 (T2), the fish were starved for four days and fed for sixteen days; in treatment 3 (T3), the fish were starved for eight days and fed for thirty-three days. The cycles were repeated eight times for the T1, four times for the T2, and two times for the T3 until the end of the experiment (80 days). The control treatment was also fed to satiety throughout the experiment. At the end of the period of starvation, the activity level of trypsin and chymotrypsin in the control treatment was significantly higher than other treatments (P<0.05). The lipase activity level in the first cycle of T1 and T2 did not significantly different from the control treatment. However, the lipase enzyme activity in the midpoint cycle of T2 was significantly lower than control (P<0.05). At the end of days 40 and 80, no significant difference was observed in the activities of three digestive enzymes between the control and the experimental treatments. The present study showed that Sparidentex hasta can cover their digestive capacity after overcoming various periods of starvation.
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کلیدواژه‌ها [English]

  • Starvation
  • Re-feeding
  • Digestive Enzymes Activity
  • Sparidentex hasta

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